Search Results 1-20 of 135

  • Elucidation of neuronal circuit mechanisms of learning and memory for developing new drugs for cognitive impairments  [in Japanese]

    Nomura Hiroshi

    … 本講演では記憶の想起を中心とした記憶・学習の神経回路機構について、in vivoでの神経活動操作やイメージングを含めた最新の知見と今後の展望を紹介したい。 …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 1-YAL3, 2021


  • Pharmacology of Baloxavir (Xofluza<sup>®</sup>); a First-in-Class Cap-dependent Endonuclease Inhibitor for Treatment of Influenza  [in Japanese]

    Takao Shishido

    … Baloxavir was approved for treatment of uncomplicated influenza A and B virus infections in 2018 in Japan (for those weighing &gt;10 kg) and the United States (for those aged 12 years and older) </p><p>Key nonclinical characteristics of baloxavir include broad spectrum activity against various types and subtypes of influenza virus strains <i>in vitro</i> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-LBS-30, 2020


  • VII. 共同利用研究, 2.研究成果, A.計画研究  [in Japanese]

    … 葉を起点とする領域間回路の解析とうつ病モデルの創出2017-A-22 マカクザル前頭極の多シナプス性ネットワークの解明2017-A-23 ヒトとチンパンジーにおける「平均」の知覚に関する比較認知研究2017-A-26 人類出現期に関わる歯と頭蓋骨の形態進化的研究2017-A-27 高等霊長類成体脳神経新生の動態と機能のin vivo解析技術の創出2017-A-28 ウイルスベクターを利用した神経回路操作技術による霊長類脳機能の解明 …

    霊長類研究所年報 = Annual Reports of the Primate Research Institute Kyoto University (48), 87-93, 2018-10-19


  • The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture

    Shioda Setsuko , Kasai Fumio , Ozawa Midori , Hirayama Noriko , Satoh Motonobu , Kameoka Yousuke , Watanabe Ken , Shimizu Norio , Tang Huamin , Mori Yasuko , Kohara Arihiro

    … Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. … Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. …

    Cytotechnology 70(1), 141-152, 2018-02


  • Genetic stability of human multipotent mesenchymal stromal cells during ex vivo expansion

    Nikitina Victoria , Samoylov Aleksandr , Astrelina Tatiana , Lomonosova Elena , Nugis Vladimir , Suchkova Yulia , Kobzeva Irina , Brunchukov Vitalyi , Usupzhanova Daria , Bushmanov Andrey

    … <p>A considerable amount of data is available that describes the effectiveness of human multipotent mesenchymal stromal cells (MSChs) in clinical practice. … Expansion of MSC is essential for therapeutic use, but it can result in genetically aberrant cells, their positive selection in the population, and their contamination of the material for transplantation. … The frequency of polyploidy in bmMSCh and atMSCh was 5.2+/-1.01% and 0%, respectively. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO4-4-5, 2018


  • The role of histidine-rich glycoprotein on immunothrombosis in septic organ failure

    Wake Hidenori , Mori Shuji , Teshigawara Kiyoshi , Liu Keyue , Wang Dengli , Gao Yuan , Takahashi Hideo K , Nishibori Masahiro

    … <p>Background: Sepsis definition was updated in 2016. … Recent studies revealed that neutrophil adhesion on vascular wall followed by neutrophil extracellular traps (NETs) release may trigger platelet aggregation and immunothrombus formation in septic organ failure. … Recent reports suggested that HRG may be associated with regulation of blood vascular function in septic condition. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO3-9-3, 2018


  • The synergistic antitumor effect of combined Trastuzumab and gamma interferon therapy to drug resistant Human Epidermal Growth Factor Receptor 2 (HER2) positive breast cancer cells

    Gocho Toshihiko , Tsuchiya Hiromichi , Kamijyo Shotaro , Yamazaki Yoshitaka , Akita Yui , Ao Yoko , Sasaki Akiko , Kiuchi Yuji

    … <p>We reported that Trastuzumab and gamma interferon combined therapy showed higher synergistic antitumor effect than usual therapy in vitro and in vivo mouse model before.</p><p> …  In this study we evaluated whether Trastuzumab and gamma interferon combined therapy shows good synergy effect to drug resistant HER2 positive human breast cancer cells or not. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO1-8-19, 2018


  • Renin-Angiotensin System Inhibitors Can Prevent Intravenous Lipid Infusion-Induced Myocardial Microvascular Dysfunction and Leukocyte Activation

    Yasu Takanori , Mutoh Akiko , Wada Hiroshi , Kobayashi Mayumi , Kikuchi Yuji , Momomura Shinichi , Ueda Shinichiro

    … <p><b><i>Background:</i></b>Levels of triglycerides and free fatty acids (FFAs) are elevated in patients with diabetes and may contribute to endothelial dysfunction through renin-angiotensin system (RAS) activation and oxidative stress. …

    Circulation Journal 82(2), 494-501, 2018

    J-STAGE  Ichushi Web 

  • In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis

    Konishi Hide A. , Asai Suguru , Watanabe Tomonobu M. , Yoshimura Shige H.

    … This protein-rich environment functions as an entropic barrier that prevents the passage of molecules, as well as the binding sites for karyopherins, to regulate macromolecular traffic between the nucleoplasm and the cytoplasm. … In this study, we expressed individual Nups fused with a crowding-sensitive probe (GimRET) to determine the spatial distribution of protein-rich domains within the central channel in vivo, and characterize the properties of the entropic barrier. …

    Scientific Reports (7), 2017-07-18


  • An <i>In Vitro</i> Contraction Model in Mouse Primary Cultured Myotubes Using Satellite Cells Originated from the EDL and Soleus Muscles


    … <p><b><i>Purpose: </i></b>Skeletal muscle cell lines such as mouse C2C12 cells and rat L6 cells often show abnormal characteristics because of repeated-passage cultures and artificial culture conditions. … Primary myotubes are considered to retain their <i>in vivo</i> …

    Juntendo Medical Journal 62(Suppl.1), 143-143, 2016


  • Triploid human embryonic stem cells derived from tripronuclear zygotes displayed pluripotency and trophoblast differentiation ability similar to the diploid human embryonic stem cells


    In the present study, a total of 20 tripronuclear zygotes were cultured; … Although the cell line displayed an abnormal chromosome number, it was found the cell line has been shown to be pluripotent with the ability to differentiate into 3 embryonic germ layers both <i>in vitro</i> … and <i>in vivo</i>. …

    Journal of Reproduction and Development 62(2), 167-176, 2016

    J-STAGE  Ichushi Web 

  • The Use of Bone Marrow Stromal Cells (Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells) for Alveolar Bone Tissue Engineering: Basic Science to Clinical Translation

    Kagami Hideaki , Agata Hideki , Inoue Minoru , Asahina Izumi , Tojo Arinobu , Yamashita Naohide , Imai Kohzoh

    … Bone tissue engineering is a promising field of regenerative medicine in which cultured cells, scaffolds, and osteogenic inductive signals are used to regenerate bone. … Although it is known that cell culture and induction protocols significantly affect the in vivo bone forming ability of BMSCs, the responsible factors of clinical outcome are poorly understood. …

    Tissue Engineering Part B: Reviews 20(3), 229-232, 2014-06-01


  • Chemiluminescence can be an indicator of the viability of transplanted neural stem cells from the rat subventricular zone

    Hirano Shun-ichiro , Fujimoto Tetsuya , Inoue Hiroshi , Uchihashi Kenji , Nishikawa Yasuo

    … The cultured cells retained their capacity for chemiluminescence, allowing us to use them in experimental transplantation. … Neither replicative senescence nor stress-induced senescence was observed during passage of the cells. …

    Journal of Osaka Dental University 48(2), 141-149, 2014

    J-STAGE  Ichushi Web 

  • Fhit Deficiency-Induced Global Genome Instability Promotes Mutation and Clonal Expansion

    Miuma Satoshi , Saldivar Joshua C. , Karras Jenna R. , Waters Catherine E. , Paisie Carolyn A. , Wang Yao , Jin Victor , Sun Jin , Druck Teresa , Zhang Jie , Huebner Kay

    … We have proposed that Fhit is a genome 'caretaker' whose loss initiates genome instability in preneoplastic lesions. …

    PLoS ONE 8(11), e80730, 2013-11-14


  • Decontamination of mycoplasma-contaminated Orientia tsutsugamushi strains by repeating passages through cell cultures with antibiotics

    Ogawa Motohiko , Uchiyama Tsuneo , Satoh Masaaki , Ando Shuji

    … Background: Mycoplasmas-contamination of Orientia tsutsugamushi, one of the obligated intracellular bacteria, is a very serious problem in in vitro studies using cell cultures because mycoplasmas have significant influence on the results of scientific studies. … Only a recommended decontamination method is to passage the contaminated O. … tsutsugamushi which are difficult to propagate in mice. …

    BMC Microbiology (13), 32, 2013-02-08


  • Generation of a replication-competent chimeric simian-human immunodeficiency virus carrying env from subtype C clinical isolate through intracellular homologous recombination.

    Fujita Yasuhisa , Otsuki Hiroyuki , Watanabe Yuji , Yasui Mika , Kobayashi Takeshi , Miura Tomoyuki , Igarashi Tatsuhiko

    … The resulting recombinant was subjected to serial passage in rhesus peripheral blood mononuclear cells (RhPBMCs). … The resulting SHIV 97ZA012 was replication competent in RhPBMCs and monkey alveolar macrophages, and possessed CCR5 preference as an entry co-receptor. … Experimental infection of rhesus macaques with SHIV 97ZA012 caused high titers of plasma viremia and a transient but profound depletion of CD4(+) T lymphocytes in the lung. …

    Virology 436(1), 100-111, 2013-02-05


  • Effect of Fulvic Acid on Ultraviolet Induced Skin Aging:The Effect of Fulvic Acid on Fibroblasts and Matrix Metalloproteinase  [in Japanese]

    KINOSHITA Hisako , KINOSHITA Masayoshi , TAKAHASHI Akiyo , YUASA Shinsuke , FUKUDA Keiichi

    皮膚老化は皮膚が人間の最も表面にある臓器ゆえに,経時的,遺伝的な内因的要因だけではなく,日光や大気などの外的要因も老化現象に影響を及ぼす。皮膚のたるみやしわの大きな原因は皮下組織の線維芽細胞の細胞数減少や結合組織構成タンパクの分泌能低下,日光などの外的ストレスが原因となるコラーゲン分解亢進などが主な原因となって起こることが知られている。フルボ酸は腐植物質より抽出される自然由来の物質であるが,キレー …

    Nishi Nihon Hifuka 74(4), 427-431, 2012


  • Effect of Skin Fibroblast-Derived Allogeneic Feeder Cells on Porcine ES-Like Cell Establishment


    In the present study, the effect of two types of allogeneic-derived feeder cells [porcine ear and tail skin fibroblasts (PESF, PTSF)] and three types of xenogeneic-derived feeder cells [human foreskin fibroblasts (HFK), mouse embryonic fibroblasts (MEF) and immortalized mouse embryonic fibroblasts (STO)] on the isolation and cultivation of putative porcine embryonic stem cells (pESCs) was evaluated. … <i>In vivo</i> …

    Journal of Veterinary Medical Science 74(10), 1243-1251, 2012

    J-STAGE  Ichushi Web 

  • Reproduction in vitro of a quasispecies from a hepatitis C virus-infected patient and determination of factors that influence selection of a dominant species

    Kawaguchi Kazunori , Faulk Kristina , Purcell Robert H. , Emerson Suzanne U.

    … 金沢大学医学系研究科Hepatitis C virus infections proceed to chronicity in the majority of cases. … In patients, hepatitis C viruses exist as a dynamic and complex quasispecies. … Hepatitis C virus infections proceed to chronicity in the majority of cases. … In patients, hepatitis C viruses exist as a dynamic and complex quasispecies. …

    Journal of Virology 85(7), 3408-3414, 2011-04-01


  • Effects of <I>Morinda citrifolia</I> extract and its constituents on blood fluidity

    Masuda Megumi , Murata Kazuya , Itoh Kimihisa , Naruto Shunsuke , Uwaya Akemi , Isami Fumiyuki , Matsuda Hideaki

    … 次に,MCL-ext と MCS-ext 投与ラットにおける <I>in vivo</I> … 今回ノニの血液流動性改善作用が初めて明らかになり,MCL-ext は MCS-ext よりも強い血小板凝集抑制作用,<I>in vivo</I> … 血流低下抑制作用, 及び <I>in vivo</I> …

    Journal of Traditional Medicines 28(2), 47-54, 2011


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