Search Results 1-20 of 119

  • Evaluation of apocynin in vitro on high glucose-induced oxidative stress on tenocytes

    Kurosawa T. , Mifune Y. , Inui A. , Nishimoto H. , Ueda Y. , Kataoka T. , Yamaura K. , Mukohara S. , Kuroda R.

    … Aims The purpose of this study was to evaluate the in vitro effects of apocynin, an inhibitor of nicotinamide adenine dinucleotide phosphate oxidase (NOX) and a downregulator of intracellular reactive oxygen species (ROS), on high glucose-induced oxidative stress on tenocytes. … Reactive oxygen species production, cell proliferation, apoptosis and messenger RNA (mRNA) expression of NOX1 and 4, and interleukin-6 (IL-6) were determined in vitro. …

    Bone & Joint Research 9(1), 23-28, 2020-01


  • Myocardial atrophy regulated by the formation of TRPC3 protein complex  [in Japanese]

    Nishida Motohiro , Tanaka Tomohiro , Nishiyama Kazuhiro , Nishimura Akiyuki

    … We have investigated the mechanism underlying induction of myocardial atrophy induced by anti-cancer drug treatment, and found that formation of protein complex between NADPH oxidase 2 (Nox2) and transient receptor potential canonical (TRPC) 3 contributed to ROS-mediated myocardial atrophy in mice. … Here, we report that extracellular adenosine 5'-triphosphate (ATP) promotes nutrient deficiency-induced cardiomyocyte atrophy through TRPC3-Nox2 complex formation. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-S31-1, 2020


  • <i>Nox1</i> deficiency alleviated behavioral abnormalities in obsessive-compulsive disorder model mice via inhibition of D<sub>2</sub> receptor/β-arrestin pathway-mediated synaptic facilitation  [in Japanese]

    Asaoka Nozomi , Kaneko Shuji , Yabe-Nishimura Chihiro

    … In this study, we showed that mRNA expression of NOX1, the catalytic subunit of NADPH oxidase, was significantly increased in the striatum of OCD model mice. … <i>Nox1</i> … deficiency or pharmacological inhibition of NOX1 alleviated OCD-like behavioral abnormalities. … <i>Nox1</i> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-P-283, 2020


  • Elucidation of ROS effects on auditory transmission impairment  [in Japanese]

    Chen Lin , Mouri Hiroaki , Saito Naoaki , Ueyama Takehiko

    … <p>【BACKGROUND】The NADPH oxidase (NOX) family consists of seven enzymes that produce reactive oxygen species (ROS). … NOX4 is expressed in various cells and tissues, and functions in many signaling pathways. … We have reported transgenic mice of NOX4 (NOX4-TG) that overproduce ROS without phenotypes in hearing loss under baseline conditions; …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-P-218, 2020


  • TRPC3-Nox2 complex formation mediates nutritional deficiency-induced cardiomyocyte atrophy  [in Japanese]

    Tanaka Tomohiro , Binti Sudi Suhaini , Oda Sayaka , Numaga-Tomita Takuro , Nishiyama Kazuhiro , Nishimura Akiyuki , Sunggip Caroline , Mangmool Superchoke , Nishida Motohiro

    … ATP led to overproduction of reactive oxygen species (ROS) through increased amount of NADPH oxidase (Nox) 2 proteins, due to increased physical interaction between Nox2 and canonical transient receptor potential 3 (TRPC3). … This ATP-mediated formation of TRPC3-Nox2 complex was also pathophysiologically involved in nutritional deficiency-induced NRCM atrophy. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-YIA-36, 2020


  • Effects of NADPH oxidase deletion in the stroke-prone spontaneously hypertensive rat  [in Japanese]

    Fujikawa Koichi , Hashizoe Yuki , Nabika Toru

    … In this study, roles of Nox2 and Nox4, which are two major subtypes of NADPH oxidases in BP regulation and cerebrovascular events were evaluated using Nox2- and Nox4-deleted stroke prone spontaneously hypertension rat (SHRSP). …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-O-064, 2020


  • NOX1/NADPH in the hypothalamus regulate anxiety-like behaviors in mice  [in Japanese]

    Ibi Masakazu , Zhang Xueqing , Yabe-Nishimura Chihiro

    NADPH oxidase is a superoxide-generating enzyme composed of multiple subunits including a membrane-spanning catalytic subunit, NOX. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-LBS-07, 2020


  • Canagliflozin Prevents Diabetes-Induced Vascular Dysfunction in ApoE-Deficient Mice

    Rahadian Arief , Fukuda Daiju , Salim Hotimah Masdan , Yagi Shusuke , Kusunose Kenya , Yamada Hirotsugu , Soeki Takeshi , Sata Masataka

    … Canagliflozin also reduced the expressions of NADPH oxidase subunits such as NOX2 and p22phox in the aorta and reduced urinary excretion of 8-OHdG, suggesting a reduction in oxidative stress. …

    Journal of Atherosclerosis and Thrombosis, 2020


  • Formation of neutrophil extracellular traps in mitochondrial DNA-deficient cells

    Takishita Yutaka , Yasuda Hiroyuki , Shimizu Mio , Matsuo Akane , Morita Akihiro , Tsutsumi Tomonari , Tsuchiya Masahiko , Sato Eisuke F.

    … Although it is known that NET formation occurs via NADPH oxidase (NOX)-dependent and NOX-independent pathways, the detailed mechanism remains unknown. … Therefore, in this study, we aimed to elucidate the role of mitochondria in NOX-dependent and NOX-independent NET formation. …

    Journal of Clinical Biochemistry and Nutrition 66(1), 15-23, 2020


  • NADPH oxidase (Nox) 5α redox signaling is required for HTLV-1-induced cancer phenotype  [in Japanese]

    鎌田 徹

    細胞 51(10), 506-509, 2019-09

  • Antimicrobial response by the superoxide-producing NADPH oxidases  [in Japanese]

    神田 朗 , 住本 英樹

    臨床免疫・アレルギー科 = Clinical immunology & allergology 72(1), 14-20, 2019-07

    Ichushi Web 

  • NF-κB-induced NOX1 activation promotes gastric tumorigenesis through the expansion of SOX2-positive epithelial cells

    Echizen Kanae , Horiuchi Keigo , Aoki Yayoi , Yamada Yoichi , Minamoto Toshinari , Oshima Hiroko , Oshima Masanobu

    Embargo Period 6 months

    Oncogene 38(22), 4250-4263, 2019-03-30

    IR  DOI 

  • Rho-Family Small GTPases: From Highly Polarized Sensory Neurons to Cancer Cells

    Ueyama Takehiko

    The small GTPases of the Rho-family (Rho-family GTPases) have various physiological functions, including cytoskeletal regulation, cell polarity establishment, cell proliferation and motility, transcri …

    Cells 8(2), 92, 2019-02


  • NOX4/NADPH oxidase regulates the expression of endoglin, a TGF-β co-receptor  [in Japanese]

    Katsuyama Masato , Arakawa Noriaki , Yabe-Nishimura Chihiro

    … <p>Reactive oxygen species (ROS) derived from NOX4/NADPH oxidase are known to play important roles in tissue fibrogenesis. … However, signaling pathways downstream of NOX4 involved in fibrogenesis have not yet been well established. … In cells transfected with siRNAs against NOX4, we found that levels of endoglin, a co-receptor of TGF-β, were reproducibly decreased compared with those in control siRNA-transfected cells. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 3-P-117, 2019


  • Identification of a novel TRPC3-Nox2 complex inhibitor that attenuates anthracycline-induced cytotoxicity  [in Japanese]

    Nishiyama Kazuhiro , Numaga-Tomita Takuro , Fujimoto Yasuyuki , Tanaka Tomohiro , Toyama Tiemi , Nishimura Akiyuki , Azuma Yasu-Taka , Nishida Motohiro

    … We previously reported that the formation of protein complex between transient receptor potential canonical 3 (TRPC3) and NADPH oxidase 2 (Nox2) mediates Dox-induced cardiac atrophy in mice. … The aim of this study was to identify a drug already approved for clinical use that blocks Dox-induced cytotoxicity, and demonstrate whether inhibition of TRPC3-Nox2 complex by this drug attenuates Dox-induced systemic tissue wasting in mice. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 2-YIA-23, 2019


  • Increased oxidative stress mediated by NOX1/NADPH oxidase promotes liver injury in a mouse model of nonalcoholic fatty liver disease  [in Japanese]

    Zhang Xueqing , Matsumoto Misaki , Zhang Jia , Yabe-Nishimura Chihiro

    … To clarify the source of oxidative stress, hepatic expression of the superoxide-generating NADPH oxidase isoforms was examined in high-fat and high-cholesterol (HFC) diet-fed mice. … The level of NOX1, but not of NOX2 or NOX4 mRNA was significantly elevated in the liver of mice fed HFC diet for 8 weeks. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 1-SS-53, 2019


  • TRPC3-Nox2 axis mediates ATP-induced cardiomyocyte atrophy  [in Japanese]

    Oda Sayaka , Sudi Binti Suhaini , Tanaka Tomohiro , Numaga-Tomita Takuro , Nishida Motohiro

    … We have recently showed that functional coupling between transient receptor potential canonical (TRPC) 3 and NADPH oxidase 2 (Nox2) mediated aberrant ROS production during pathological remodeling of mouse hearts. … In this study, we found that extracellular ATP caused atrophy of neonatal rat cardiomyocytes (NRCMs), which was attenuated by silencing TRPC3 or Nox2 genes. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 1-SS-36, 2019


  • A Protective role of NOX1/NADPH oxidase against cytotoxic components of cigarette smoke extracts in cardiomyocyte  [in Japanese]

    Wen Xiaopeng , Iwata Kazumi , Yabe-Nishimura Chihiro

    … <p>A superoxide-generating NADPH oxidase (NOX) has been reported to mediate cytotoxicity induced by cigarette smoke components. … However, the underlying molecular mechanisms and NOX isoform involved have not been fully clarified. … Among NOX isoforms identified so far, NOX1 and NOX4 were expressed in rat H9c2 cardiomyocytes. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 1-SS-38, 2019


  • NOX1 Negatively Modulates Fibulin-5 in Vascular Smooth Muscle Cells to Affect Aortic Dissection

    Hu Xiaoping , Jiang Wanli , Wang Zhiwei , Li Luocheng , Hu Zhipeng

    … Reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 1 (NOX1) deficiency reduces the incidence of aortic dissection induced by angiotensin II, but its mechanism remains to be further elucidated. … This study was to clarify the relationship between NOX1 and Fibulin-5 in the AD. … Results showed that the expressions of NOX1 and Fibulin-5 were increased and decreased in the AD, respectively. …

    Biological and Pharmaceutical Bulletin 42(9), 1464-1470, 2019

    J-STAGE  Ichushi Web 

  • Hearing vulnerability after noise exposure in a mouse model of reactive oxygen species overproduction

    Morioka Shigefumi , Sakaguchi Hirofumi , Yamaguchi Taro , Ninoyu Yuzuru , Mohri Hiroaki , Nakamura Takashi , Hisa Yasuo , Ogita Kiyokazu , Saito Naoaki , Ueyama Takehiko

    … To resolve this issue, we established an in vivo model of ROS overproduction by generating a transgenic (TG) mouse line expressing the human NADPH oxidase 4 (NOX4, NOX4-TG mice), which is a constitutively active ROS-producing enzyme that does not require stimulation or an activator. … Overproduction of ROS was detected at the cochlea of the inner ear in NOX4-TG mice, but they showed normal hearing function under baseline conditions. …

    Journal of Neurochemistry 146(4), 459-473, 2018-08


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