Search Results 1-20 of 96

  • 1 / 5
  • Atriplex nummularia extract suppresses RANKL-activated osteoclastogenesis  [in Japanese]

    KO Tsui Ling , KUO Han Chun , YANG Jyh Ferng , CHANG Chi Chang , WANG Shih Wei , HOUNG Jer Yiing

    … ANE significantly inhibited RANKL-induced activation of NFATc1, TRAF6, NF-κB, JNK, ERK, p38 and MMP-9 during osteoclast differentiation from RAW264.7 cells. …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-201, 2020

    J-STAGE 

  • Detection of RANKL-producing cells and osteoclastic activation by the addition of exogenous RANKL in the regenerating scales of goldfish

    Yamamoto Tatsuki , Urata Makoto , Hattori Atsuhiko , Suzuki Nobuo , Ikegame Mika , Kawago Umi , Tabuchi Yoshiaki , Hirayama Jun , Sekiguchi Toshio , Furusawa Yukihiro , Yachiguchi Koji , Matsubara Hajime

    … In addition, treating the regenerating scales with mammalian RANKL for 3 h significantly increased the expression of the nuclear factor of activated T cells, cytoplasmic 1 (NFATc1), which is essential for osteoclast differentiation. …

    Biological Sciences in Space 34(0), 34-40, 2020

    J-STAGE 

  • Fluorinated Kavalactone Inhibited RANKL-Induced Osteoclast Differentiation of RAW264 Cells

    Kumagai Momochika , Nishikawa Keisuke , Mishima Takashi , Yoshida Izumi , Ide Masahiro , Watanabe Akio , Fujita Kazuhiro , Morimoto Yoshiki

    … downregulated mRNA expression levels of RANKL-induced nuclear factor of activated T cells c1 (NFATc1) and osteoclastogenesis-related genes. …

    Biological and Pharmaceutical Bulletin 43(5), 898-903, 2020

    J-STAGE 

  • Mechanism of action of pyrogallol on calcineurin-NFAT signaling  [in Japanese]

    Mizuguchi Hiroyuki , Kitamura Yoshiaki , Takeda Noriaki , Fukui Hiroyuki , Nakano Tomohiro , Nishida Kouhei , Ito Tomohiro , Wakugawa Tomoharu , Kaminuma Osamu , Kitamura Noriko , Ishida Tatsuya , Yabumoto Masami

    … Pull-down assay revealed that pyrogallol strengthened interaction between NFATc1 and calcineurin. … Further studies demonstrated that calcineurin binding site 2 in NFATc1 was involved in pyrogallol's effect. Poly(U)-binding-splicing factor 60 (PUF60) was identified as NFATc2 binding protein using pyrogallol-immobilized affinity chromatography. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-O-005, 2020

    J-STAGE 

  • Elucidation of osteoporosis prevention mechanism by citrus limonoid

    君羅 好史

    … These collaborations were necessary, as Kimira explains: 'I'm not a specialist in food processing, so collaborating with companies specialising in food processing was very important.' The team's project is entitled 'Citrus limonoid nomilin inhibits osteoclastogenesis in vitro by suppression of NFATc1 and MAPK signalling pathways'. …

    Impact 2019(6), 12-14, 2019-06

    IR 

  • MicroRNA-124 inhibits TNF-α- and IL-6-induced osteoclastogenesis

    Ohnuma Kenichiro , Kasagi Shimpei , Uto Kenichi , Noguchi Yoriko , Nakamachi Yuji , Saegusa Jun , Kawano Seiji

    … MicroRNA (miR)-124 has been known to suppress RANKL-dependent osteoclastogenesis by inhibiting NFATc1 expression. … We also showed that the expression levels of osteoclast-specific genes and NFATc1 protein were suppressed in the miR-124 mimic-transfected cells by performing quantitative-polymerase chain reaction and western blotting. … Our results indicate that miR-124 is important in inhibiting both RANKL-dependent and -independent osteoclast differentiation by suppressing NFATc1-mediated pathway. …

    Rheumatology International 39(4), 689-695, 2019-04

    IR 

  • KBTBD11, a novel BTB-Kelch protein, is a negative regulator of osteoclastogenesis through controlling Cullin3-mediated ubiquitination of NFATc1

    Narahara Shun , Sakai Eiko , Kadowaki Tomoko , Yamaguchi Yu , Narahara Haruna , Okamoto Kuniaki , Asahina Izumi , Tsukuba Takayuki

    … Among six major signaling pathways regulating osteoclast differentiation, KBTBD11 predominantly influenced the nuclear factor of activated T cell cytoplasmic-1 (NFATc1) pathway. … Further experiments involving immunoprecipitation and treatment with MG132, a proteasome inhibitor,showed that the KBTBD11–Cullin3 promotes ubiquitination and degradation of NFATc1 by the proteasome. …

    Scientific Reports 9(1), 3523, 2019-03-05

    IR 

  • G12/G13 biology in energy metabolism  [in Japanese]

    Kim Sg , Jh Koo , Th Kim , Ym Yang , Sy Park , Ms Joo , Cy Han , Cs Choi

    … Mechanistically, Gα13 and its downstream effector RhoA suppressed nuclear factor of activated T cells 1 (NFATc1), a chief regulator of myofiber conversion, by increasing Rock2-mediated phosphorylation at Ser243. … Ser243 phosphorylation of NFATc1 was reduced after exercise, but was higher in obese animals. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), JKG-01, 2019

    J-STAGE 

  • S100A12 facilitates osteoclast differentiation from human monocytes

    Nishida Miwa , Saegusa Jun , Tanaka Shino , Morinobu Akio

    … S100A12 facilitated osteoclast formation in the presence of RANKL, as judged by the cells'morphology and elevated expression of osteoclast-related molecules, including NFATc1, ACP5, CALCR, and ITGβ3. …

    PLoS ONE 13(9), e0204140, 2018-09-20

    IR 

  • Resolvin E1 Inhibits Osteoclastogenesis and Bone Resorption by Suppressing IL-17-induced RANKL Expression in Osteoblasts and RANKL-induced Osteoclast Differentiation

    Funaki Yoshihiro , Makino Haruhiko , Shimizu Eiji , Hasegawa Yasuyuki , Okazaki Ryota , Yamasaki Akira , Sueda Yuriko , Yamamoto Akihiro , Yanai Masaaki , Fukushima Takehito , Harada Tomoya

    … The effects of RvE1 on the RANKL-induced mRNA expression of osteoclast-specific genes and transcriptional factors such as c-fos and nuclear factor of activated T cells c1 (NFATc1) in RAW264.7 cells were measured by quantitative real-time PCR. … The distribution of NFATc1 induced by RANKL was evaluated by immunofluorescence staining in RAW264.7 cells. … RvE1 inhibited the RANKL-induced mRNA expression of osteoclast-specific genes along with the transcription factors NFATc1 and c-fos. …

    Yonago Acta Medica 61(1), 008-018, 2018

    IR  J-STAGE  Ichushi Web 

  • <b>Dihydroartemisinin represses osteoclastogenesis of bone marrow macrophages through reduced NFATc1 expression and impaired phosphorylation of </b><b>IκBα </b>

    KOMAKI Shunsuke , SAKAI Eiko , FUKUMA Yutaka , NISHISHITA Kazuhisa , OKAMOTO Kuniaki , TSUKUBA Takayuki

    … Moreover, DHA treatment induced down-regulation of nuclear factor of activated T cells cytoplasmic-1 (NFATc1), a master regulator for osteoclast differentiation and its target proteins, such as Src and cathepsin K. … These results indicate that DHA represses RANKL-induced osteoclastogenesis of bone marrow macrophages through reduced NFATc1 expression and impaired phosphorylation of IκBα.</p> …

    Biomedical Research 39(4), 169-177, 2018

    J-STAGE  Ichushi Web 

  • <i>Sargassum serratifolium</i> attenuates RANKL-induced osteoclast differentiation and oxidative stress through inhibition of NF-κB and activation of the Nrf2/HO-1 signaling pathway

    Kim Hong Jae , Park Cheol , Kim Gi-Young , Park Eui Kyun , Jeon You-Jin , Kim Suhkmann , Hwang Hye Jin , Choi Yung Hyun

    … EESS also attenuated RANKL-induced expressions of key osteoclast-specific genes, such as nuclear factor of activated T cells cytoplasmic 1 (NFATC1), TRAP, cathepsin K and matrix metalloproteinase-9. …

    BioScience Trends 12(3), 257-265, 2018

    J-STAGE  Ichushi Web 

  • Potential Role of Rebamipide in Osteoclast Differentiation and Mandibular Condylar Cartilage Homeostasis

    Izawa Takashi , Hutami Islamy Rahma , Tanaka Eiji

    … Regarding the latter, reduced expression of cathepsin K, NFATc1, c-Src, and integrin β3, and increased expression of nuclear factor-kappa B, have been found to be mediated by the transcription factor, receptor activator of nuclear factor kappa-B ligand (RANKL).Methods: Treatment with rebamipide was also found to activate, mitogen-activated protein kinases such as p38, ERK, and JNK to reduce osteoclast differentiation. …

    Current Rheumatology Reviews 14(1), 62-69, 2018

    IR 

  • The effects of marsupialization on bone regeneration adjacent to keratocystic odontogenic tumors, and the mechanisms involved

    Hu Xiang , Zhao Yi , Man Qi-Wen , Li Rui-Fang , Liu Bing , Zhao Yi-Fang

    … Real-time qPCR also demonstrated increased expression of OPG after marsupialization, accompanied by a decrease in the expression of osteoclastogenesis-related molecules such as cathepsin K, matrix metalloproteinase-9, NFATc1, RANK and RANKL. …

    Journal of Oral Science 59(4), 475-481, 2017

    J-STAGE  Ichushi Web 

  • Glucosamine Suppresses Osteoclast Differentiation through the Modulation of Glycosylation Including <i>O</i>-GlcNAcylation

    Takeuchi Tomoharu , Sugimoto Aya , Imazato Nao , Tamura Mayumi , Nakatani Sachie , Kobata Kenji , Arata Yoichiro

    … GlcN supplementation suppressed the upregulation of osteoclast-specific genes (tartrate-resistant acid phosphatase (<i>TRAP</i>), cathepsin K, matrix metallopeptidase 9, and nuclear factor of activated T cell c1 (<i>NFATc1</i>)), receptor activator of nuclear factor-κB ligand (RANKL)-dependent upregulation of TRAP enzyme activity, and the formation of TRAP-positive multinuclear cells more effectively than <i>N</i>-acetylglucosamine (GlcNAc), which we have previously shown to inhibit osteoclast differentiation. …

    Biological and Pharmaceutical Bulletin 40(3), 352-356, 2017

    IR  J-STAGE  Ichushi Web 

  • Rebamipide Attenuates Mandibular Condylar Degeneration in a Murine Model of TMJ-OA by Mediating a Chondroprotective Effect and by Downregulating RANKL-Mediated Osteoclastogenesis

    Izawa Takashi , Mori Hiroki , Shinohara Takehiro , Mino-Oka Akiko , Hutami Islamy Rahma , Iwasa Akihiko , Tanaka Eiji

    … Rebamipide also suppressed the activation of transcription factors (e.g., NF-κB, NFATc1) and mitogen-activated protein kinases (MAPK) by receptor activator of nuclear factor kappa-B ligand (RANKL) to inhibit the differentiation of osteoclastic precursors, and disrupted the formation of actin rings in mature osteoclasts. …

    PLOS ONE 11(4), e0154107, 2016-04-28

    IR 

  • NUCLEAR FACTOR OF ACTIVATED T CELLS (NFAT) INDUCES BLADDER CANCER CELL PROLIFERATION  [in Japanese]

    河原 崇司

    … 免疫応答に関わる転写因子であるNFATc 1 が正常組織に比べ各種癌組織で発現が上昇しているとの報告が散見される.また諸説あるものの,疫学調査において腎不全による人工透析患者に比べ腎移植後に免疫抑制剤を服用していた患者の方が膀胱癌発生率が低下したと報告されている.しかしながら,そのメカニズムについては不明であった.NFATc 1 は免疫抑制剤の主要標的であることから,免疫抑制 …

    横浜医学 = Yokohama medical journal 67(1), 59-61, 2016-01

    IR  Ichushi Web 

  • 荷重除去とその後の再荷重によるマウスヒラメ筋の筋線維タイプ移行とNFATファミリーの発現  [in Japanese]

    横山 真吾 , 大野 善隆 , 後藤 勝正

    … また,Western blot法によりNFATc1-4の発現量を評価した。 … ヒラメ筋におけるNFATc1発現量は後肢懸垂により約3倍に増加し(p<0.01),再荷重によって減少して後肢懸垂前の水準まで回復した。 … 【結論】タイプI MyHC発現には,NFATc1-4の全ての関与が示唆されている。 …

    Congress of the Japanese Physical Therapy Association 2015(0), 0466, 2016

    J-STAGE 

  • P1-05 TankyraseによるSH3BP2を介した破骨細胞分化調節の検討  [in Japanese]

    向井 知之 , 藤田 俊一 , 三戸 崇史 , 長洲 晶子 , 平野 紘康 , 守田 吉孝

    … blot法にてSH3BP2,NFATc1発現を解析した.【結果】RAW264.7細胞および骨髄由来マクロファージ培養系ともに,Tankyrase阻害薬によりRANKL誘導性破骨細胞形成が亢進し,破骨細胞関連遺伝子発現および骨吸収活性も有意に増強した.この作用はWnt阻害薬では認めなかった.Tankyrase阻害薬により,細胞内SH3BP2は有意に増加し,核におけるNFATc1発現は有意に増加していた.NFATc1阻害薬(FK506) …

    Japanese Journal of Clinical Immunology 39(4), 377a-377a, 2016

    J-STAGE  Ichushi Web 

  • Suppression of osteoclastogenesis via upregulation of Zfyve21 and Ddit4 by salubrinal and guanabenz  [in Japanese]

    HAMAMURA KAZUNORI , TANJUNG NANCY , CHEN ANDY , YOKOTA HIROKI , TOGARI AKIFUMI

    … initiation factor 2 alpha(eIF2<i>α</i>)の脱リン酸化阻害剤として知られていて,それらは,破骨細胞分化のマスター分子であるnuclear factor of activated T-cells, cytoplasmic 1(NFATc1)を低下させ,破骨細胞分化を抑制する.NFATc1の抑制メカニズムはあまり解明されていない.そこで,ゲノムワイドのマイクロアレー解析を用いて,私たちは,特にsalubrinalおよびguanabenzが作用する破骨細胞分化に関与す …

    ORAL THERAPEUTICS AND PHARMACOLOGY 35(3), 127-135, 2016

    J-STAGE 

  • 1 / 5
Page Top