Search Results 1-20 of 44

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  • A screening for DNA damage response molecules that affect HIV-1 infection

    Yoshinaga Noriyoshi , Shindo Keisuke , Matsui Yusuke , Takiuchi Yoko , Fukuda Hirofumi , Nagata Kayoko , Shirakawa Kotaro , Kobayashi Masayuki , Takeda Shunichi , Takaori-Kondo Akifumi

    RAD18 −/− cells showed twofold higher HIV-1 infectivity and increased reverse transcription products at earlier time points, suggesting that RAD18 suppresses reverse transcription. … The HIV-1 suppressive effects of RAD18 were confirmed by over-expression and knockdown experiments in human cells. …

    Biochemical and Biophysical Research Communications 513(1), 93-98, 2019-05-21

    IR 

  • NBS1 and multiple regulations of DNA damage response

    Komatsu Kenshi

    … NBS1 binds to at least five key proteins, including ATM, RPA, MRE11, RAD18 and RNF20, in the conserved regions within a limited span of the C terminus, functioning in the regulation of chromatin remodeling, cell cycle checkpoint and DNA repair in response to DSBs. …

    Journal of Radiation Research 57(S1), i11-i17, 2016-08-16

    IR 

  • Role of RAD18 on IR-induced DNA damage signaling pathways  [in Japanese]

    笹谷 めぐみ , 徐 衍賓 , 河合 秀彦 , 飯塚 大輔 , 神谷 研二

    広島医学 69(4), 284-286, 2016-04

    Ichushi Web 

  • Functional role of NBS1 in radiation damage response and translesion DNA synthesis

    Saito Yuichiro , Komatsu Kenshi

    … In addition to DSB response, we showed that NBS1 initiates Polη-dependent translesion DNA synthesis by recruiting RAD18 through its binding at the NBS1 C-terminus after UV exposure, and it also functions after the generation of interstrand crosslink DNA damage. …

    Biomolecules 5(3), 1990-2002, 2015-08-20

    IR 

  • Role of E3 ubiquitin ligase RAD 18 on radioresponse  [in Japanese]

    徐 衍賓 , 笹谷 めぐみ , 河合 秀彦 [他]

    Nagasaki Igakkai zasshi = Nagasaki medical journal 89(特集号), 303-305, 2014-09

    Ichushi Web 

  • Role of NBS1 in DNA damage response and its relationship with cancer development

    Saito Yuichiro , Fujimoto Hiroko , Kobayashi Junya

    … The N-terminal FHA/BRCT domains interact with MDC1, γ-H2AX, TopBP1, and WRN, and the C-terminal motifs are indispensable for binding MRE11, ataxia telangiectasia mutated (ATM), RAD18, and RNF20. … NBS1 also participates in the regulation of RAD18/Polη-dependent translesion DNA synthesis (TLS) through its interaction with RAD18 and WRN. …

    Translational Cancer Research 2(3), 178-189, 2013-06

    IR 

  • Molecular mechanisms underlying ochratoxin A-induced genotoxicity : global gene expression analysis suggests induction of DNA double-strand breaks and cell cycle progression

    HIBI Daisuke , KIJIMA Aki , KURODA Ken , SUZUKI Yuta , ISHII Yuji , JIN Meilan , NAKAJIMA Masahiro , SUGITA KONISHI Yoshiko , YANAI Tokuma , NOHMI Takehiko , NISHIKAWA Akiyoshi , UMEMURA Takashi

    … Interestingly, genes that were deregulated in the OM possessed molecular functions such as DNA double-strand break (DSB) repair (<i>Rad18</i>, <i>Brip1</i>, and<i> …

    The Journal of Toxicological Sciences 38(1), 57-69, 2013-02-01

    J-STAGE  Ichushi Web  References (52)

  • 複製後修復機構が放射線損傷応答におよぼす寄与 (特集 第53回原子爆弾後障害研究会講演集)  [in Japanese]

    徐 衍賓 , 笹谷 めぐみ , 河合 秀彦 [他] , 増田 雄司 , 神谷 研二 ,

    … 誘導される.PCNAのユビキチン化を担う因子の1つにRAD6-RAD18複合体がある2).RAD6または,RAD18が欠損するとモノユビキチン化とポリユビキチン化の両方が消失することから,RAD6-RAD18複合体によるPCNAのユビキチン化が損傷乗り越えDNA合成およびテンプレートスイッチの両者を制御していると考えられる.実際に,RAD18遺伝子を欠失したヒト培養細胞では,化学物質や放射線 …

    Nagasaki Igakkai zasshi = Nagasaki medical journal 87(特集号), 202-204, 2012-09

    Ichushi Web 

  • In Vitro Studies of Exchanges between Replicative and Translesion DNA Polymerases in the Eukaryotic Post-replication Repair Pathway

    MASUDA Yuji

    … The mono-ubiquitination of proliferating cell nuclear antigen (PCNA) by the RAD6-RAD18 complex is involved in the regulation of translesion DNA synthesis, which is one of the sub-pathways of post-replication repair. …

    Genes and Environment 34(2), 70-76, 2012-05-20

    J-STAGE  Ichushi Web  References (55)

  • Human DNA Polymerase η and Its Regulatory Mechanisms

    MASUTANI Chikahide

    … and PCNA, TLS polymerases, RAD18 and DNA repair proteins, as well as their posttranslational modifications, have been shown to be important for regulating Polη.<br> …

    Genes and Environment 34(2), 63-69, 2012-05-20

    J-STAGE  Ichushi Web  References (83)

  • NBS1 Recruits RAD18 via a RAD6-like Domain and Regulates Pol η-Dependent Translesion DNA Synthesis.

    Yanagihara Hiromi , Kobayashi Junya , Tateishi Satoshi , Kato Akihiro , Matsuura Shinya , Tauchi Hiroshi , Yamada Kouichi , Takezawa Jun , Sugasawa Kaoru , Masutani Chikahide , Hanaoka Fumio , Weemaes Corry M , Mori Toshio , Zou Lee , Komatsu Kenshi

    … While the ubiquitin-conjugating enzyme RAD6 and ubiquitin ligase RAD18 are known to monoubiquitinate PCNA, how they are regulated by DNA damage is not fully understood. … We show that NBS1 (mutated in Nijmegen breakage syndrome) binds to RAD18 after UV irradiation and mediates the recruitment of RAD18 to sites of DNA damage. … Disruption of NBS1 abolished RAD18-dependent PCNA ubiquitination and Polη focus formation, leading to elevated UV sensitivity and mutation. …

    Molecular cell 43(5), 788-797, 2011-09-02

    IR  Cited by (2)

  • NBS1 regulates translesion DNA synthesis.  [in Japanese]

    YANAGIHARA Hiromi , TATEISHI Satoshi , YAMADA Koichi , MORI Toshio , KOBAYASHI Junya , KOMATSU Kenshi

    … Moreover NBS1-deficient cells showed reduced RAD18 and Pol eta focus formation, and severely reduced mono-ubiquitination of PCNA. … We show that NBS1 binds to RAD18 after UV irradiation and mediates the recruitment of RAD18 to sites of DNA damage. … Disruption of NBS1 abolished RAD18-dependent PCNA ubiquitination and Pol eta focus formation, leading to elevated UV sensitivity and mutation. …

    The Japan Radiation Research Society Annual Meeting Abstracts 2011(0), 96-96, 2011

    J-STAGE 

  • Roles of 53BP1 and Rad18 in fusion of unprotected telomeres  [in Japanese]

    HASHIMOTO Mitsumasa , MATSUI Tadashi , HASHIMOTO Yumi , TATEISHI Satoshi , IWABUCHI Kuniyoshi

    … We found that (1) 53BP1 plays a role in a novel pathway distinct from the Ku-dependent and Artemis-dependent non-homologous end-joining (NHEJ) pathways (Genes Cells, 11: 935, 2006), and that (2) RAD18 interacts with 53BP1 and is recruited at DSB sites in a 53BP1-dependent manner specifically during the G1-phase of the cell cycle. … RAD18 monoubiquitinates 53BP1and enhances retention of 53BP1 in chromatin (Nucleic Acids Res, 37: 2176, 2009). …

    The Japan Radiation Research Society Annual Meeting Abstracts 2011(0), 203-203, 2011

    J-STAGE 

  • Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A–RAD18 ternary complex

    Masuda Yuji , Suzuki Miki , Kawai Hidehiko , Suzuki Fumio , Kamiya Kenji

    RAD18, a RING-type ubiquitin ligase (E3) that plays an essential role in post-replication repair, possesses distinct domains named RING, UBZ, SAP and the RAD6-binding domain (R6BD) and forms a dimer. … In this study, we established a method to distinguish between the two subunits of RAD18 by introduction of different tags, and analyzed mutant complexes. …

    Nucleic Acids Research 40(3), 1065-1076, 2011

    IR 

  • WRN participates in translesion synthesis pathway through interaction with NBS1.

    Kobayashi Junya , Okui Michiyo , Asaithamby Aroumougame , Burma Sandeep , Chen Benjamin P C , Tanimoto Keiji , Matsuura Shinya , Komatsu Kenshi , Chen David J

    … WS cells exhibit an increase in spontaneous focus formation of poleta and Rad18, which are important for translesion synthesis (TLS). … Moreover, WS cells show constitutive ubiquitination of PCNA and interaction between PCNA and Rad18 E3 ligase in the absence of DNA damage. …

    Mechanisms of ageing and development 131(6), 436-444, 2010-06

    IR 

  • Spontaneous tumor formation in Rad18 knockout mouse  [in Japanese]

    TATEISHI Satoshi , ETOH Mizuna

    Rad18 is a key molecule of the mechanism. … Rad18 directs mono-ubiquitination of PCNA to regulate cellular localization of translesion polymerase. … To investigate role of Rad18 on tumor prevention, we prepared Rad18 knockout mouse and examined spontaneous tumor frequency. … Next, we compared Rad18 expression level of transformed human cell lines and cell lines derived from tumor patients with those of primary human cells. …

    The Japan Radiation Research Society Annual Meeting Abstracts 2010(0), 111-111, 2010

    J-STAGE 

  • The role of NBS1 in Translesion DNA Synthesis  [in Japanese]

    YANAGIHARA Hiromi , MORI Toshio , TATEISHI Satoshi , KOBAYASHI Junya , KOMATSU Kenshi

    … Moreover UV-induced PCNA mono-ubiquitination and Rad18,pol eta foci were not observed in NBS cells. …

    The Japan Radiation Research Society Annual Meeting Abstracts 2010(0), 226-226, 2010

    J-STAGE 

  • Physical and functional interaction between WRNIP1 and RAD18

    YOSHIMURA Akari , SEKI Masayuki , KANAMORI Makoto , TATEISHI Satoshi , TSURIMOTO Toshiki , TADA Shusuke , ENOMOTO Takemi

    … Genetic studies in budding yeast suggested that the yeast orthlog of WRNIP1, Mgs1, may function in a DNA damage tolerance pathway that is similar to, but distinct from, the template-switch damage avoidance pathway involving Rad6, Rad18, Rad5, Mms2, and Ubc13. … We found that WRNIP1 interacts physically with RAD18 and interferes with the binding of RAD18 to forked DNA and to template/primer DNA. …

    Genes & Genetic Systems 84(2), 171-178, 2009-04-25

    J-STAGE  Ichushi Web  References (34)

  • The role of NBS1 for cellular response after UV-C exposure

    YANAGIHARA Hiromi , MORI Toshio , TATEISHI Satoshi , KOBAYASHI Junya , KOMATSU Kenshi

    … Moreover UV-induced PCNA mono-ubiquitination and Rad18, pol eta foci were not observed in NBS cells. …

    The Japan Radiation Research Society Annual Meeting Abstracts 2009(0), 117-117, 2009

    J-STAGE 

  • Biochemical analysis of DNA polymerase switching on the post-replication repair pathway

    MASUDA Yuji , KAMIYA Kenji

    … Post-replication repair pathway (PRR) protects cells from a wild variety of DNA damage and it is regulated by <i>RAD18</i> … Indeed, <i>RAD18</i> … In eukaryotes, RAD6-RAD18 dependent mono-ubiquitination at the lysine 164 residue of proliferating cell nuclear antigen (PCNA) is deemed to play a key role in regulation of TLS. …

    The Japan Radiation Research Society Annual Meeting Abstracts 2009(0), 99-99, 2009

    J-STAGE  Ichushi Web 

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