Search Results 1-20 of 3261

  • Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium

    ANTONOULI Sevastiani , NOTTOLA Stefania Annarita , MACCHIARELLI Guido , PALMERINI Maria Grazia , BIANCHI Serena , ROSSI Gianna , CECCONI Sandra , BELLI Manuel , BERNARDI Sara , KHALILI Mohammad Ali , FAMILIARI Giuseppe

    … The absence of cilia, necessary to sustain oviductal transport of oocytes, spermatozoa and embryos, may originate from either mitochondrial dysfunction or glycogen consumption. …

    Journal of Reproduction and Development, 2020

    J-STAGE 

  • Development of assisted reproductive technologies in small animal species for their efficient preservation and production

    MOCHIDA Keiji

    … fertilization (IVF), the cryopreservation of embryos and spermatozoa, transportation of cryopreserved materials and embryo transfer (ET). …

    Journal of Reproduction and Development, 2020

    J-STAGE 

  • Genetic confirmation of "egg parasitism" in androgenetic freshwater <i>Corbicula</i> clams by paternity testing using microsatellite DNA markers

    Sano Natsumi , Houki Shouji , Kodan Ayaka , Kawamura Kouichi , Yamada Mitsuya , Komaru Akira

    … In these species, outcrossing means "egg parasitism" by the spermatozoa from one or more other clams, because they reproduce by androgenesis in which only the nucleus of spermatozoa is transmitted to the progeny. …

    Plankton and Benthos Research 15(1), 58-62, 2020

    J-STAGE 

  • Production of mouse offspring from inactivated spermatozoa using horse PLCζ mRNA

    YAMAMOTO Yunosuke , HIROSE Naoki , KAMIMURA Satoshi , WAKAYAMA Sayaka , ITO Junya , OOGA Masatoshi , WAKAYAMA Teruhiko

    … Here, we examined whether intracytoplasmic injection of equine sperm-specific phospholipase C zeta (ePLCζ) mRNA, the PLCζ with the strongest oocyte activation potential in mammals, could improve the mouse oocyte activation rate and subsequent embryonic development using inactivated spermatozoa. … Full-term development was examined using NaOH-treated inactive spermatozoa using the optimal activation method. …

    Journal of Reproduction and Development 66(1), 67-73, 2020

    J-STAGE 

  • Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation

    ISHINO Takeshi , KURITA Hirofumi , KIRISAWA Rikio , SHIMAMOTO Yoshinori , NUMANO Rika , KITAMURA Hiroshi

    … Flow cytometry analysis revealed that 71.5% of spermatozoa exhibited green fluorescence after transfection. …

    Journal of Veterinary Medical Science 82(1), 14-22, 2020

    J-STAGE 

  • The Mroh4 (maestro heat-like repeat family member 4) gene affects spermatogenesis and sperm morphogenesis in mice

    井尻 貴之 , 近藤 玄 , 堀江 恭二 , 竹田 潤二 , 松田 洋一

    摂南大学 融合科学研究所論文集 = Bulletin of the Transdisciplinary and Interdisciplinary Science Research Institute Setsunan University 5(1), 41-56, 2019-12

    IR 

  • Function of the sperm acrosomal EQUATORIN protein analyzed using genetically modified mice  [in Japanese]

    年森 清隆 , 伊藤 千鶴

    Journal of mammalian ova research 36(2), 71-77, 2019-10

    Ichushi Web 

  • Male mice, caged in the International Space Station for 35 days, sire healthy offspring

    村谷 匡史 , 工藤 崇 , 高橋 智 , Takafumi Matsumura , Taichi Noda , Masafumi MURATANI , Risa Okada , Mutsumi Yamane , Ayako Isotani , Takashi KUDO , Satoru TAKAHASHI , Masahito Ikawa

    … The cauda epididymal spermatozoa from AG and MG mice could fertilize oocytes in vitro at comparable levels as GC males. … When the fertilized eggs were transferred into pseudo-pregnant females, there was no significant difference in pups delivered (pups/transferred eggs) among GC, AG, and MG spermatozoa. …

    Scientific Reports 9(1), 13733, 2019-09

    IR 

  • Identification and characterization of superoxide dismutase in silkworm seminal fluid

    Nagaoka Sumiharu , Yamamoto Kohji

    Journal of insect biotechnology and sericology 88(2), 39-47, 2019-06

  • Study of bovine sperm motility in shear-thinning viscoelastic fluids

    Hyakutake Toru , Sato Koichi , Sugita Kenta

    … To elucidate the process whereby sperm arrive at an egg in the female reproductive organs, it is essential to investigate how rheological properties of the fluid around mammalian spermatozoa affect their motility. … The results indicated that the surrounding rheological properties largely affected the flagellar waveform of mammalian spermatozoa; …

    Journal of Biomechanics (88), 130-137, 2019-05-09

    IR 

  • Recovery of Spermatogenesis Following Cancer Treatment with Cytotoxic Chemotherapy and Radiotherapy

    Okada Keisuke , Fujisawa Masato

    … Sperm cryopreservation is an effective method for preserving spermatozoa. …

    World Journal of Mens Health 37(2), 166-174, 2019-05

    IR 

  • Small, Variable Sperm in a Barn Swallow Population with Low Extra-Pair Paternity

    Hasegawa Masaru , Arai Emi , Nakamura Masahiko

    Zoological science 36(2), 154-158, 2019-04

  • Effects of 5-hydroxytryptamine on spermatozoal hyperactivation and <i>in vitro</i> fertilization in mice

    SUGIYAMA Yukiko , FUJINOKI Masakatsu , SHIBAHARA Hiroaki

    … In this study, we examined the effects of 5-hydroxytryptamine (5-HT) on the motility and hyperactivation of mouse spermatozoa. … receptors significantly increased the percentage of hyperactivated spermatozoa but did not affect the percentage of motile spermatozoa. … receptors significantly affected the velocities, linearity, straightness, wobbler coefficient, amplitude and/or frequency of spermatozoa. …

    Journal of Reproduction and Development 65(6), 541-550, 2019

    J-STAGE 

  • Study on facilitating fertility for breeding of red foxes in captivity  [in Japanese]

    Yatsu M.

    <p> 宮城蔵王キツネ村に展示飼育されているアカキツネを用いて,受胎率の向上と遺伝的多様性の維持を目的に検討を行った.まず,使用する雌雄アカキツネが健康であることを確認するために,バイタルサイン,血液検査,凝固検査および血清生化学検査を行い基準値(範囲)を求めた.犬の既知参考値と比較して,直腸温,赤血球数,好酸球数,血清グルコース,アルブミンおよび尿素窒素濃度は高値,一方平均赤血球容積 …

    Japanese Journal of Large Animal Clinics 10(3), 105-115, 2019

    J-STAGE 

  • Development of a bovine sperm selection procedure for improvement of livestock fertility  [in Japanese]

    NAGATA MariaPortia B. , YAMASHITA Kenichi

    家畜の繁殖性改善は、畜産業の生産性向上による食糧供給の安定化とともに、地方の産業振興やバイオエコノミーとしての位置づけなど、幅広い意義を持つ。我々は、牛の繁殖性改善のため、手薄とされる精子側の研究に取り組んだ。研究開発の方向性は、ヒトの不妊治療にかかる報告を参考にして、健全性の高い精子は、運動性も高いという点に着目し、運動している精子を周囲の溶液に流れを生じさせて集合させるという技術を開発すること …

    Synthesiology 12(2), 75-83, 2019

    J-STAGE 

  • Early production of offspring by <i>in vitro</i> fertilization using first-wave spermatozoa from prepubertal male mice

    MOCHIDA Keiji , HASEGAWA Ayumi , OGONUKI Narumi , INOUE Kimiko , OGURA Atsuo

    … Because we noted that the addition of reduced glutathione (GSH) to the IVF medium significantly increased the fertilizing ability of spermatozoa from prepubertal males, we used this IVF protocol for all experiments. … Spermatozoa first reached the caudal region of the epididymides at day 35; … Caudal epididymal spermatozoa first became competent for oocyte fertilization at day 37, albeit at a low rate (2.9%). …

    Journal of Reproduction and Development 65(5), 467-473, 2019

    J-STAGE 

  • Methylation status in IVF embryos of nuclear DNA methylation variable sites related with spermatozoa fertility  [in Japanese]

    TAKEDA Kumiko , WATANABE Shinya , KOBAYASHI Eiji , NISHINO Kagetomo , HOSHINO Yoichiro , ADACHI Hiromichi , IMAI Akira , IWAO Ken , OGATA Kazuko , KANEDA Masahiro

    <p>【目的】我々は,牛凍結精液について採取雄牛の加齢に伴う変化*や受胎性と関連しうる精子核DNAのメチル化可変部位の探索を行い,それら精子核DNAのメチル化可変部位のメチル化率の違いを利用した評価法の開発を目指している。本研究では,受胎性に関連しうるメチル化可変部位について人工授精後の受胎率や体外受精後の胚発生率の異なる凍結精液を用いた体外受精胚におけるメチル化状態について調査したの …

    The Journal of Reproduction and Development Supplement 112(0), P-35-P-35, 2019

    J-STAGE 

  • Characterization of SPACA1 proteins in the spermatozoa from Japanese Black bulls  [in Japanese]

    MINAMI Kenta , ASO Miyuki , YAMADA Ayano , KISHIDA Kazumi , SAKASE Mitsuhiro , FUKUSHIMA Moriyuki , HARAYAMA Hiroshi

    <p>【目的】活力良好なウシ精子を用いた人工授精において,受胎率が著しく低くなるような症例(低繁殖症)が報告されている。このような低繁殖症の雄個体を検出するための手法として,精子の正常性を分子レベルで検査する方法(分子性状検査法)の開発が試みられている。本研究では,黒毛和種精子のSPACA1タンパク質が分子性状検査法のマーカーとして有効であるか否かについて検討し,ついでこのタンパク質の …

    The Journal of Reproduction and Development Supplement 112(0), P-38-P-38, 2019

    J-STAGE 

  • Analysis of the mechanism of impaired capacitation in Slc22a14-KO mouse spermatozoa  [in Japanese]

    UNOU Masato , HIGUCHI Toshiya , SO Shuhei , ITO Masahiko , YOGO Keiichiro

    <p>【目的】受精能獲得は精子が受精するために必須であり,重炭酸イオン・カルシウムイオンの流入,コレステロールの除去等によって引き起される。近年我々は,精子鞭毛主部に局在する有機アニオン/カチオントランスポーターSlc22a14を欠損したマウス精子は,運動性や受精能獲得能が低下することを見出した。また,Slc22a14欠損精子では,細胞内pHが低下していること,受精能獲得時の重炭酸イオ …

    The Journal of Reproduction and Development Supplement 112(0), P-21-P-21, 2019

    J-STAGE 

  • Production of offspring from freeze-dried mouse spermatozoa preserved on the sheets  [in Japanese]

    ITO Daiyu , WAKAYAMA Sayaka , OOGA Masatoshi , WAKAYAMA Teruhiko

    <p>【目的】従来,凍結乾燥精子は真空状態が保たれたガラスアンプルでの保存が大前提であったが,アンプル瓶の作製ミスや破損などの懸念が伴う。本研究では,凍結乾燥精子をアンプル瓶よりも安全に長期間保存できる新たな保存媒体と最適処理方法の探索,及びその技術を用いた産仔の作出を試みた。【方法】実験にはICR系統のマウス精子と卵子を用いた。保存媒体には薬包紙,オブラート,プラスチックシート,ラッ …

    The Journal of Reproduction and Development Supplement 112(0), P-128-P-128, 2019

    J-STAGE 

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