Search Results 1-20 of 542

  • Development of Beat Force Analysis Software for Non-invasive Evaluation of the Cardiac Muscle Cell Constructs  [in Japanese]

    TESHIMA Chihiro , SHIMOTO Takeshi , ARAI Kenichi , NAKAYAMA Koichi

    … <p>Our research group developed a method for arranging spheroids in arbitrary positions to form cell constructs; … If the cardiac muscle cell was used, the cell construct beats. … This study aimed to develop software that can analyse the beat force and timing of the cell constructs by measuring the displacement of the tip of a needle. …

    The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec) 2020(0), 2P2-F11, 2020

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  • Immunohistochemical analysis of periostin in the hearts of Lewis rats with experimental autoimmune myocarditis

    CHOI Yuna , OH Hanseul , AHN Meejung , KANG Taeyoung , CHUN Jiyoon , SHIN Taekyun , KIM Jeongtae

    … Western blot analysis revealed that the expression levels of periostin and alpha-smooth muscle actin were significantly increased at day 14 post-immunization. … In conclusion, these results suggest that increased periostin expression in macrophages and fibroblasts promotes cardiac fibrosis in EAM-induced rats, potentially by enhancing immune cell infiltration. …

    Journal of Veterinary Medical Science 82(10), 1545-1550, 2020

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  • A Fatal Case of Myocarditis Following Myositis Induced by Pembrolizumab Treatment for Metastatic Upper Urinary Tract Urothelial Carcinoma

    Matsui Hotaka , Akazawa Hiroshi , Suzuki Motofumi , Komuro Issei , Kume Haruki , Kawai Taketo , Sato Yusuke , Ishida Junichi , Kadowaki Hiroshi , Akiyama Yoshiyuki , Yamada Yuta , Nakamura Masaki , Yamada Daisuke

    … Physical examination revealed reduced muscle reflexes and proximal muscle weakness. … A marked elevation of cardiac enzymes, including CK, myoglobin, and cardiac troponin I, was detected. … Myocardial biopsy revealed inflammatory cell infiltration and the partial impairment of myocardial tissue. … The electromyogram was normal, but inflammation in myofibers was noted in a muscle biopsy. …

    International Heart Journal 61(5), 1070-1074, 2020

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  • Functional improvement of contractile force properties of human iPS cell-derived cardiac cell sheets by continuous stimulation for several days  [in Japanese]

    TANIGUCHI Tomohiko , HINATA Yuto , KAGAWA Yuki , KATO Eriko , BABA Atsushi , KUBO Hirotsugu , YOSHINAGA Takashi , SAWADA Kohei

    … Assessments of cardiotoxicity risk like cardiac contractility are mostly conducted during the late phase of preclinical development using in vivo animal models. …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-160, 2020

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  • Inflammatory myopathy associated with immune checkpoint inhibitors  [in Japanese]

    Seki Morinobu

    … A variety of studies have demonstrated that myositis as an irAE is often accompanied by ocular muscle symptoms, which physicians have often termed "myasthenia-like" or "pseudo-myasthenic" symptoms. … To comprehensively characterize the inflammatory myopathy associated with programmed cell death 1 inhibitors (PD–1 myopathy), we studied 19 Japanese patients with PD–1 myopathy. … The initial manifestation of muscle weakness was ptosis in 10 patients. …

    Neurological Therapeutics 37(2), 146-151, 2020

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  • A Novel Treatment for Arrhythmias via the Control of the Degradation of Ion Channel Proteins

    Miake Junichiro

    … The Kv1.5 channel is a potassium ion channel expressed in atrial muscle, belongs to the voltage-gated K<sup>+</sup> … It is important to understand the fate of these channel proteins, as cardiac Kv1.5 mutations can cause arrhythmias. … As a result, ion channel proteins are not transported to the cell membrane and are involved in the development of atrial fibrillation. …

    Yonago Acta Medica 63(3), 146-153, 2020

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  • Cardio-oncology — elucidation of the mechanism of cardiac dysfunction caused by cancer therapy and cancer cachexia  [in Japanese]

    Nonaka Miki , Ueno Susumu , Uezono Yasuhito

    … However, the relationship between cancer cachexia and cardiac dysfunction remains unclear, due to lack of suitable models. … We established a novel murine model of cancer cachexia by implantation of 85As2 cells, a cell line derived from human gastric cancer cells, presenting anorexia, weight loss and low fat-free mass similar to those observed in patients. … Moreover, cardiac dysfunction is expected in this model, which has not been yet examined. …

    Folia Pharmacologica Japonica 155(3), 165-170, 2020

    J-STAGE  Ichushi Web 

  • A novel mouse model of coronary stenosis mimicking Kawasaki disease induced by <i>Lactobacillus casei</i> cell wall extract

    Suganuma Eisuke , Sato Satoshi , Honda Satoko , Nakazawa Atsuko

    … <p>Kawasaki disease (KD), a febrile systemic vasculitis in infants associated with coronary aneurysm, is a major cause of cardiac sequelae such as myocardial infarction (MI) and sudden death. … cell wall extract (LCWE). … In addition, frequent elastin degradation was observed and abundant α-smooth muscle actin (SMA)-positive vascular smooth muscle cells (VSMCs) infiltrated into the intima. …

    Experimental Animals 69(2), 233-241, 2020

    J-STAGE  Ichushi Web 

  • Cell motility and role of ACTC1 in migration of malignant glioma cells

    Wanibuchi Masahiko

    … In this article, the mechanism of cell motility isreviewed, focusing on actin, alpha cardiac muscle 1 (ACTC1) which is one of the six isoforms of actin.</p> … On the contrary, the amoeboid migration is induced by the formation of a bleb, formed by contraction of actin-myosin complex in the cytoplasm, close to the cell membrane. …

    Progress in Neuro-Oncology 27(1), 1-4, 2020

    J-STAGE  Ichushi Web 

  • Voluntary wheel running improves cardiac dysfunction associated with cancer cachexia induced by human stomach cancer cell line 85As2  [in Japanese]

    Nonaka Miki , Ueno Susumu , Kakigi Ryo , Kurebayashi Nagomi , Murayama Takashi , Miyano Kanako , Terawaki Kiyoshi , Sakurai Takashi , Uezono Yasuhito

    … However, the relationship between cancer cachexia and cardiac dysfunction remains unclear, due to lack of suitable models. … We established a novel murine model of cancer cachexia by implantation of human stomach cancer cell line 85As2, which represent anorexia, weight loss and low fat-free mass similar to those observed in cancer patients. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-O-128, 2020

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  • The Na/Ca exchange was affected by KB-R7943, nicorandil and monensin in breast cancer cells  [in Japanese]

    Matsushita Natsuko , Kuraji Maki , Ookubo Akari , Takatori Yuki , Maeda Ayaha , Kimura Yuki , Shimizu Yoshiko , Takahashi Rei , Tsuchida Katsuharu

    … NCX has been studied in a variety of cells, e.g. cardiac myocytes, smooth muscle cells, neurons, and recently, cancer cells as well. … flux and cell proliferation. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-P-355, 2020

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  • Cardiovascular Safety Tests for the Promotion of Drug Development in Academia  [in Japanese]

    Naito Atsuhiko

    … >Cardiac toxicity is the leading cause of attrition during drug development. International Consortium on Harmonization provides the guideline for evaluating the potential arrhythmogenicity of drug candidates based on <i>in vitro</i> assessment of I<sub>Kr</sub> inhibition by hERG test and <i>in vivo</i> measurement of QT interval.</p><p>The purpose of this study is to support the drug development in Japanese non-profit research institutions through undertaking the cardiac

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-S19-3, 2020

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  • Doxorubicin induces trans-differentiation in cardiac fibroblasts via cell death-independent pathways  [in Japanese]

    Umemura Masanari , Tanaka Ryo , Narikawa Masatoshi , Nakakaji Rina , Rafikul Islam , Osawa Kohei , Hikichi Mayu , Ishikawa Yoshihiro

    … The effect of DOX on cardiac fibroblasts at non-lethal concentrations remains unknown. … The aim of this study was to investigate the direct effect of doxorubicin on the activation of cardiac fibroblasts independent of cell death pathways. … Human cardiac fibroblasts (HCFs) were used. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-S22-1, 2020

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  • Effect of Bcl-2 associated athanogene (BAG) 3 on cardiac disease in alpha-B crystallin R120G transgenic mouse  [in Japanese]

    Sanbe Atsushi , Ojima Mika , Higashio Rieko , Hirose Masamichi

    … <p>It is known that Bcl-2 associated athanogene (BAG) 3 is strongly expressed in cardiac muscle as well as skeletal muscle. … Recent study showed that myofibrillar degeneration, disruption of Z-disk architecture and apoptotic cell death were observed in BAG3 knockout mouse. … Little is known, however, detail roles of the increased BAG3 in cardiac muscle. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-P-209, 2020

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  • Production of functional antibody targeting Ca<sup>2+</sup>permeable channel TRPV2 ameliorating dilated cardiomyopathy in animal models  [in Japanese]

    Iwata Yuko , Ito Shin , Wakabayashi Shigeo , Kitakaze Masafumi

    … In this study, we produced selective antibodies recognizing TRPV2 from the outside of cell. … Intraperitoneal administration of the antibody (0.5 mg/kg) for 2 weeks (once a week) prevented the progression of cardiac dysfunction as evaluated by echocardiography and improved the abnormal Ca<sup>2+</sup> … Interestingly, endogenous TRPV2 accumulated in the cardiac muscle sarcolemma disappeared upon antibody administration. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-P-065, 2020

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  • Developmental changes in sarcoplasmic reticulum (SR) dependency of contraction and relaxation mechanisms in the mouse ventricular myocardium  [in Japanese]

    Hamaguchi Shogo , Nagahara Kota , Namekata Iyuki , Tanaka Hikaru

    … t-Tubules, which were absent in the cell center region until 1 week, were present throughout the cytoplasm after 2 weeks. … at the cell center showed slower rise than the subsarcolemmal region, but after 2 weeks, Ca<sup>2+</sup> … increased simultaneously across the entire width of the cell. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-P-070, 2020

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  • Elucidation of expression and regulation mechanism of cell adhesion factor Gicerin/CD146 in cardiac hypertrophy model  [in Japanese]

    Obara Mami , Sato Sachiko , Takahashi Kumi , Kondo Yukiko , Hirose Masamichi , Nata Koji , Taira Eichi

    … <p>Gicerin/CD146 is a cell adhesion molecule which belongs to the immunoglobulin (Ig) superfamily. … We speculate that in the heart, Gicerin/CD146 may also have some role in the hypertrophy of the cardiac muscle cells. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-P-067, 2020

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  • CRISPR-Knockout Screen Identifies Dmap1 as a Regulator of Chemically Induced Reprogramming and Differentiation of Cardiac Progenitors

    Yu Jason S. L. , Palano Giorgia , Lim Cindy , Moggio Aldo , Drowley Lauren , Plowright Alleyn T. , Bohlooly-Y Mohammad , Rosen Barry S. , Hansson Emil M. , Wang Qing-Dong , Yusa Kosuke

    … Direct in vivo reprogramming of cardiac fibroblasts into myocytes is an attractive therapeutic intervention in resolving myogenic deterioration. … Current transgene‐dependent approaches can restore cardiac function, but dependence on retroviral delivery and persistent retention of transgenic sequences are significant therapeutic hurdles. …

    STEM CELLS 37(7), 958-972, 2019-07

    IR 

  • Tyrosine kinase FYN inhibition mediates the therapeutic effects of Eicosapentaenoic acid on pulmonary hypertension  [in Japanese]

    Kurahara Hai Lin , Hiraishi Keizo , Yamamura Aya , Zhang Ying , Shioi Narumi , Inoue Ryuji

    … Proliferation of human pulmonary artery smooth muscle cells (HPASMCs) derived from PAH patients were evaluated by the MTT assay. … EPA and RvE1 suppressed Src family activity by modulating it's autophosphorylation level.</p><p><b>Summary</b>: EPA significantly improved PAH-associated pathophysiology and cardiac dysfunction, which is likely mediated at least in part via Fyn inhibition. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 3-S23-3, 2019

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  • <i>Ophiocordyceps sinensis</i> may ameliorate cardiovascular remodeling in pulmonary arterial hypertension via TRPM7 inhibition  [in Japanese]

    Hiraishi Keizo , Kurahara Lin Hai , Yue Lixia , Yamamura Aya , Feng Jianlin , Abe Kotaro , Satoh Toru , Okada Yasumasa , Inoue Ryuji

    … experiments, OCS suppressed a TRPM7 channel activity and TGF-β2-induced endothelial-to-mesenchymal transition (EndoMT) in human pulmonary arterial endothelial cells, and abnormally enhanced proliferation of pulmonary arterial smooth muscle cells from PAH patients. … experiments using PAH model rats and mice showed that OCS ameliorated the development of pulmonary artery thickening, cardiac fibrosis and right ventricle hypertrophy with normalization of heightened right ventricle pressure. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 3-P-057, 2019

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