Search Results 1-20 of 1541

  • N-Linked Glycosylation on Anthrax Toxin Receptor 1 Is Essential for Seneca Valley Virus Infection

    Nadishka Jayawardena , Linde A. Miles , Laura N. Burga , Charles Rudin , Matthias Wolf , John T. Poirier , Mihnea Bostina

    … However, the exact role of ANTXR1 glycosylation on SVV attachment and cellular entry was unknown. … Here we show that N-linked glycosylation in the ANTXR1 vWA domain is necessary for SVV attachment and entry. … Cell blocking, co-immunoprecipitation, and plaque formation assays confirmed that deglycosylation of ANTXR1 prevents SVV attachment and subsequent entry. …

    Viruses 13(5), 769, 2021-04-28


  • Epidermal cell-patterning genes of the stem parasitic plant Cuscuta campestris are involved in the development of holdfasts

    Sultana Sabrina , Fujiwara Daiki , Aoki Koh

    Plant biotechnology 38(1), 47-56, 2021-03

  • Expression of SARS‐CoV‐2 entry factors in human oral tissue

    Sawa Yoshihiko , Ibaragi Soichiro , Okui Tatsuo , Yamashita Junro , Ikebe Tetsuro , Harada Hiroyuki

    … It was found that ACE2 is expressed in the cytoplasm and on the cell membrane mainly in the stratum granulosum of the epithelia while the TMPRSS2 is strongly expressed on the cell membrane mainly in the stratum granulosum and stratum spinosum, but not in the stratum basale. … The oral cavity can be considered an important organ for SARS‐CoV‐2 attachment and may provide a preventive medical avenue to guard against COVID‐19 by preventing saliva from scattering. …

    Journal of Anatomy 238(6), 1341-1354, 2021-01-09


  • <i>In vitro</i> investigation of the cell compatibility and antibacterial properties of titanium treated with calcium and ozone

    TAKECHI Masaaki , TSURU Kanji , TAKAMOTO Megumi , NINOMIYA Yoshiaki , ONO Shigehiro , MIZUTA Kuniko , NAKAGAWA Takayuki , SHIGEISHI Hideo , OHTA Kouji , ISHIKAWA Kunio

    … <p>The purpose of this study was to evaluate the surface modification of calcium ions on roughened titanium as a surface treatment of dental implants for cell attachment, growth, and initial bacterial adhesion. …

    Dental Materials Journal 40(3), 712-718, 2021


  • Morphology of placentome in Korean water deer <i>Hydropotes inermis argropus</i>

    SOHN Joon Hyuk , YAMANE Shota , SAITOH Yukiko , KUSAKABE Ken Takashi , KIMURA Junpei , KISO Yasuo

    … Therefore, these results suggest that the Korean water deer may possess special mechanisms or structures at the fetus attachment site to maintain this unusally high number of fetuses.</p> …

    Journal of Veterinary Medical Science, 2021


  • Development, characterization and potential applications of a multicellular spheroidal human blood-brain barrier model integrating three conditionally immortalized cell lines

    Kitamura Keita , Umehara Kenta , Ito Ryo , Yamaura Yoshiyuki , Komori Takafumi , Morio Hanae , Akita Hidetaka , Furihata Tomomi

    … In this study, we report on the development of a human conditionally immortalized cell-based multicellular spheroidal BBB (hiMCS-BBB) model. … After being seeded into non-attachment culture wells, HASTR/ci35 (astrocytes) and HBPC/ci37 cells (brain pericytes) self-assemble to form a spheroid core that is then covered with an outer monolayer of HBMEC/ci18 cells (brain microvascular endothelial cells). …

    Biological and Pharmaceutical Bulletin, 2021


  • Cardiac Fibroblasts Play Pathogenic Roles in Idiopathic Restrictive Cardiomyopathy

    Tsuru Hirofumi , Ueno Takayoshi , Miyashita Yohei , Kioka Hidetaka , Asano Yoshihiro , Sawa Yoshiki , Ozono Keiichi , Ishida Hidekazu , Narita Jun , Ishii Ryo , Suginobe Hidehiro , Ishii Yoichiro , Wang Renjie , Kogaki Shigetoyo , Taira Masaki

    … Cardiac fibroblasts (CFs) are a major cell population in the heart; … There were no significant alterations in cell proliferation, apoptosis, migration, activation, and attachment. …

    Circulation Journal 85(5), 677-686, 2021


  • Epidermal cell-patterning genes of the stem parasitic plant <i>Cuscuta campestris</i> are involved in the development of holdfasts

    Sultana Sabrina , Fujiwara Daiki , Aoki Koh

    … In this study, we assessed the roles of epidermal cell-patterning genes in the development of a holdfast. … Epidermal cell-patterning genes of <i>C. … is an upstream regulator of other epidermal cell-patterning genes. … was not upregulated after attachment to the host, and was not repressed by <i>CcJKD</i>-silencing. …

    Plant Biotechnology 38(1), 47-56, 2021


  • A novel coating layer on zirconia using modified zinc phosphatizing method

    KODAMA Kouta , VALANEZHAD Alireza , KHODAEI Mohammad , SAFAEE Sirus , JAFARNIA Shiva , NESABI Mahdis , ABE Shigeaki , WATANABE Ikuya , MURATA Hiroshi

    … MC3T3-E1 cells attachment was evaluated by SEM, and cell proliferation were assessed using MTS assay™. … cell study indicated that the coated sample had better cell morphology and proliferation. …

    Dental Materials Journal, 2021


  • Expression of α-L-Arabinofuranosidase Genes at Ripening Initiation Potentially Contributes to the Difference in Flesh Juiciness Between Processing and Fresh Tomatoes

    Miyohashi Fumika , Sawada Yukihisa , Kaminishi Aiko , Soga Ayaka , Yoshida Makoto , Kamiyoshihara Yusuke , Tateishi Akira

    … <p>Reconstruction of cell wall polysaccharide is necessary for fruit development and ripening. … Arabinose is the one of neutral sugars constituting wall polysaccharides and arabinose-containing polysaccharides play an important role in cellular attachment. …

    The Horticulture Journal 90(1), 130-137, 2021


  • Polyfunctional Nanofibril Appendages Mediate Attachment, Filamentation, and Filament Adaptability in Leptothrix cholodnii

    久能 樹 , 豊福 雅典 , 岩崎 憲治 , 野村 暢彦 , ウタダ アンドリュー シンイチ , Tatsuki KUNO , Kana Morinaga , Shinya Sugimoto , Shun Miyazaki , Masanori TOYOFUKU , Kenji IWASAKI , Nobuhiko NOMURA , Andrew Shinichi UTADA

    … Prior to the formation of this brittle microtube, Leptothrix cells secrete hair-like structures from the cell surface, called nanofibrils, which develop into a soft sheath that surrounds the filament. … We show that in SP-6 nanofibrils are important for attachment and their distribution on young filaments post-attachment is correlated to the directionality of filament elongation. …

    ACS nano 14(5), 5288-5297, 2020-05


  • PKN1 controls the aggregation, spheroid formation, and viability of mouse embryonic fibroblasts in suspension culture

    Mehruba Mona , Siddique Salman Mahmud , Mukai Hideyuki

    … The role of protein kinase N1 (PKN1) in cell aggregation and spheroid formation was investigated using mouse embryonic fibroblasts (MEFs) deficient in kinase activity caused by a point mutation (T778A) in the activation loop. … Wild type (WT) MEFs formed cell aggregates within a few hours in suspension cultures placed in poly-2-hydroxyethylmethacrylate (poly-HEMA) coated flat-bottom dishes. …

    Biochemical and Biophysical Research Communications 523(2), 398-404, 2020-03


  • Numerical Study of Air Flow in a Hemispheric Head Cyclone Separator for Fuel Cell Vehicles  [in Japanese]

    FURUKAWA Ryuji , TERADA Keisuke , FUKUZATO Shomaru , MUNEKATA Mizue , YOSHIKAWA Hiroyuki , ONO Kenji , WATANABE Takashi

    … <p>The final goal of this study is to design an optimized cyclone separator for fuel cell vehicles based on the industrially developed semispherical head cyclone separator, which has higher collection performance than the conventional flat head cyclone separator. … The size of cyclone separator for fuel cell vehicles is limited and smaller than the industrial model. …

    The Proceedings of Mechanical Engineering Congress, Japan 2020(0), S05418, 2020


  • <i>In vitro</i> evaluation of bone grafting materials on macrophage polarization, osteogenic differentiation, and the potential as bone morphogenetic protein-2 carriers  [in Japanese]

    FUJIOKA-KOBAYASHI Masako , KONO Michihide , NAGAI Takahiro , KATAGIRI Hiroki , IIZUKA Tateyuki

    …  Cell attachment/proliferation on bone grafting materials and the gene expressions of cell differentiation markers were investigated in the human leukemia monocytic cell line THP-1-derived macrophages and mouse stromal ST-2 cells, when cultured on the three different grafting materials of 1) demineralized freeze-dried bone allografts (DFDBA)(DBX<sup>®</sup>, Synthes Inc. USA), 2) deproteinized bovine bone mineral (DBBM)(Bio-Oss<sup>®</sup>, Geistlich …

    Japanese Journal of Oral and Maxillofacial Surgery 66(12), 586-595, 2020


  • Visualizing Molecular Chaperone Controlled Resilient Cell Traction Force by Micropost Arrays Fabricated by Two-Photon Initiated Polymerization

    Hayasaki Saaya , Shimizu Miho , Katsurada Yuuki , Sakai Atsushi , Yanagisawa Miho , Atomi Yoriko , Watanabe Toshiyuki

    … When the expression of one of the molecular chaperones, <i>α</i>B-crystallin was knocked down, the cells kept wandering showing round-shaped contours confirming the significance of <i>α</i>B-crystallin in maintaining subcellular attachment to extracellular matrix. …

    Journal of Fiber Science and Technology 76(9), 288-295, 2020


  • Kinetic Analysis for Agglomeration-Flotation of Finely Ground Chalcopyrite: Comparison of First Order Kinetic Model and Experimental Results

    Hornn Vothy , Ito Mayumi , Yamazawa Ryosuke , Shimada Hiromasa , Tabelin Carlito Baltazar , Jeon Sanghee , Park Ilhwan , Hiroyoshi Naoki

    … <p>Particle size-flotation rate relationships can be discussed by a first-order kinetic model for flotation, which considers the probability of particle-bubble collision, attachment and detachment; … The conventional first-order kinetic model does not consider particle size changes during flotation while the experimental results suggested that the size of agglomerates increased in the flotation cell. …

    MATERIALS TRANSACTIONS 61(10), 1940-1948, 2020


  • Development of microfluidic cell culture system in preparation for human organ on a chip  [in Japanese]

    Takahashi Ken , Wang Mengxue , Liu Yun , Liang Yin , Wang Chen , Naruse Keiji

    … After fixation of the cells with paraformaldehyde, dual staining of platelet endothelial cell adhesion molecule-1 (PECAM-1), a marker for endothelial cells, and 4',6-diamidino-2-phenylindole, a stain for nucleus, was performed. … Immunofluorescence corresponds to PECAM-1 at the cell-cell junction was confirmed under confocal microscope. … On the other hand, attachment of the cells can be improved by optimizing the coating procedure of extracellular matrix proteins. …

    Transactions of Japanese Society for Medical and Biological Engineering Annual58(Abstract), 341-341, 2020


  • An introduction of biological performance of zirconia with different surface characteristics: A review

    HAN Aifang , TSOI James K. H. , LUNG Christie Y. K. , MATINLINNA Jukka P.

    … We have included an initial tissue cell attachment study on zirconia and bacterial adhesion on zirconia. … The modifications also result in advanced biological performance of zirconia, including enhanced tissue cell attachment and reduction of bacterial adhesion. …

    Dental Materials Journal 39(4), 523-530, 2020


  • Effect of titanium or zirconia implant abutments on epithelial attachments after ultrasonic cleaning

    Hu Jiangqi , Atsuta Ikiru , Ayukawa Yasunori , Zhou Tianren , Narimatsu Ikue , Koyano Kiyoshi

    … In addition, titanium and zirconia disks were cleaned using an ultrasonic scaler, surface morphology changes were observed, and the number of epithelial cell attachments to the surface was measured. … There was no difference in the number of epithelial cell attachments between the two materials with the ultrasonic cleaning. … In conclusion, zirconia is harder than titanium and a better choice for use in the epithelial tissue attachment. …

    Journal of Oral Science 62(3), 331-334, 2020


  • Factors Affecting Frontal Sinus Surgery Anterior to the Ethmoid Bulla  [in Japanese]

    Hiraga Yoshihiko , Araki Yasutomo , Tsuzuki Nobuyoshi , Sahara Sosuke , Hashimoto Yosuke , Kawasaki Taiji , Ogawa Kaoru

    … 内視鏡下副鼻腔手術 (endoscopic sinus surgery: ESS) において, 近年は先に篩骨胞を開放せずに, agger nasi cell(ANC) を開放した後に排泄路を篩骨胞前壁の上方にたどって前頭洞を開放する方法である篩骨胞温存前頭洞開放術 (intact bulla frontal sinusotomy: IBFS) も行われている. … Supra bulla frontal cell(SBFC) が存在する症例では IBFS の完遂率は52% (11/21) で, SBFC なしでは93% (41/44) であった (p=0.0003). …

    Nippon Jibiinkoka Gakkai Kaiho 123(5), 356-362, 2020

    J-STAGE  Ichushi Web 

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