Search Results 1-20 of 1370

  • Neutralizing antibody-dependent and -independent immune responses against SARS-CoV-2 in cynomolgus macaques.

    ISHIGAKI Hirohito , NAKAYAMA Misako , KITAGAWA Yoshinori , NGUYEN Cong Thanh , HAYASHI Kaori , SHIOHARA Masanori , GOTOH Bin , ITOH Yasushi , 石垣 宏仁 , 仲山 美沙子 , 北川 善紀 , 林 香里 , 塩原 正規 , 後藤 敏 , 伊藤 靖

    … We examined the pathogenicity of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in cynomolgus macaques for 28 days to establish an animal model of COVID-19 for the development of vaccines and antiviral drugs. … Cynomolgus macaques infected with SARS-CoV-2 showed body temperature rises and X-ray radiographic pneumonia without life-threatening clinical signs of disease. …

    Virology (554), 97-105, 2021-02

    IR 

  • Vascular smooth muscle lipofuscinosis occurring predominantly in veins of a cynomolgus monkey (<i>Macaca fascicularis</i>)

    SEKIYA Akio , KAWASAKO Kazufumi , DOI Takuya

    … <p>A 6-year-old male cynomolgus monkey showed chronic wasting. …

    Journal of Veterinary Medical Science, 2021

    J-STAGE 

  • Efficacy of Neuraminidase Inhibitors against H5N6 Highly Pathogenic Avian Influenza Virus in a Nonhuman Primate Model.

    NGUYEN Cong Thanh , SUZUKI Saori , ITOH Yasushi , ISHIGAKI Hirohito , NAKAYAMA Misako , HAYASHI Kaori , MATSUNO Keita , OKAMATSU Masatoshi , SAKODA Yoshihiro , KIDA Hiroshi , OGASAWARA Kazumasa , 鈴木 紗織 , 伊藤 靖 , 石垣 宏仁 , 仲山 美沙子 , 林 香里 , 小笠原 一誠

    … H5N6 HPAIV infection led to high fever in cynomolgus macaques. … This study also showed pathologically severe lung injury states in cynomolgus macaques infected with H5N6 HPAIV, even in those that received early antiviral drug treatments, indicating the need for close monitoring and further studies on virus pathogenicity and new antiviral therapies. …

    Antimicrobial agents and chemotherapy 64(7), 2020-06-23

    IR 

  • Human disease modeling with genetically-modified cynomolgus monkey, a nonhuman primate  [in Japanese]

    依馬 正次 , 岡村 永一

    Precision medicine = プレシジョンメディシン 3(6), 568-570, 2020-06

    Ichushi Web 

  • Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein.

    SEITA Yasunari , MORIMURA Toshifumi , WATANABE Naoki , IWATANI Chizuru , TSUCHIYA Hideaki , NAKAMURA Shinichiro , SUZUKI Toshiharu , YANAGISAWA Daijiro , TSUKIYAMA Tomoyuki , NAKAYA Masataka , OKAMURA Eiichi , MUTO Masanaga , EMA Masatsugu , NISHIMURA Masaki , TOOYAMA Ikuo , 清田 弥寿成 , 守村 敏史 , 渡邊 直希 , 岩谷 千鶴 , 土屋 英明 , 中村 紳一郎 , 柳沢 大治郎 , 築山 智之 , 中家 雅隆 , 岡村 永一 , 武藤 真長 , 西村 正樹 , 遠山 育夫

    … Here we report the generation of two transgenic cynomolgus monkeys overexpressing the human gene for APP with Swedish, Artic, and Iberian mutations, and demonstrated expression of gene tagged green fluorescent protein marker in the placenta, amnion, hair follicles, and peripheral blood. …

    Journal of Alzheimer's disease : JAD 75(1), 45-60, 2020-06

    IR 

  • Characterization of tumour-infiltrating lymphocytes in a tumour rejection cynomolgus macaque model.

    SATOOKA Hiroki , ISHIGAKI Hirohito , TODO Kagefumi , TERADA Koji , AGATA Yasutoshi , ITOH Yasushi , OGASAWARA Kazumasa , HIRATA Takako , 里岡 大樹 , 石垣 宏仁 , 藤堂 景史 , 寺田 晃士 , 縣 保年 , 伊藤 靖 , 小笠原 一誠 , 平田 多佳子

    … Here, we characterize TILs in a cynomolgus macaque tumour model in which the tumours were infiltrated with CD4+ and CD8+ T cells and were eventually rejected. …

    Scientific Reports 10(1), 8414, 2020-05-21

    IR 

  • Establishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocysts

    Matsumoto Shoma , Porter Christopher J. , Ogasawara Naomi , Iwatani Chizuru , Tsuchiya Hideaki , Seita Yasunari , Chang Yu-Wei , Okamoto Ikuhiro , Saitou Mitinori , Ema Masatsugu , Perkins Theodore J. , Stanford William L. , Tanaka Satoshi

    … In this study, we have established five trophoblast stem cell (TSC) lines from cynomolgus monkey blastocysts, named macTSC #1-5. …

    Scientific Reports (10), 2020-04-22

    IR 

  • Low replicative fitness of neuraminidase inhibitor-resistant H7N9 avian influenza a virus with R292K substitution in neuraminidase in cynomolgus macaques compared with I222T substitution.

    SUZUKI Saori , SHICHINOHE Shintaro , ITOH Yasushi , NAKAYAMA Misako , ISHIGAKI Hirohito , MORI Yuya , Ogata-Nakahara Ayako , NGUYEN Cong Thanh , OKAMATSU Masatoshi , SAKODA Yoshihiro , KIDA Hiroshi , OGASAWARA Kazumasa , 鈴木 紗織 , 七戸 新太郎 , 仲山 美沙子 , 石垣 宏仁 , 森 優也 , 小笠原 一誠

    Human cases of H7N9 influenza A virus infection have been increasing since 2013. The first choice of treatment for influenza is neuraminidase (NA) inhibitors (NAIs), but there is a concern that NAI-re …

    Antiviral research (178), 104790, 2020-04-06

    IR 

  • Central Nervous System Migration of Astaxanthin and Adonixanthin Following Their Oral Administration in Cynomolgus Monkeys

    NAKAMURA Shinsuke , MAOKA Takashi , TSUJI Shohei , HAYASHI Masahiro , SHIMAZAWA Masamitsu , HARA Hideaki

    Cynomolgus monkeys were orally administered astaxanthin or adonixanthin at a dose of 50 mg/kg for 10 d, through a disposable catheter inserted into the stomach via the nasal passage. …

    Journal of Nutritional Science and Vitaminology 66(5), 488-494, 2020

    J-STAGE 

  • Roles of <i>GRHL</i> gene family in cynomolgus monkey trophoblast stem cell  [in Japanese]

    TASHIRO Akiyumi , MATSUMOTO Shoma , TANAKA Satoshi

    <p>【目的】栄養膜幹(TS)細胞は,マウス胚盤胞の栄養外胚葉から初めて樹立され,胎盤発生機構を解明するツールとして広く用いられてきた。近年,当研究室ではカニクイザルの胚盤胞からTS細胞(macTS細胞)の樹立に成功した(Matsumotoら,2020)。ヒトTS細胞およびmacTS細胞においては,マウスTS細胞の分化能維持に重要な転写因子ネットワークを構成するCDX2やELF5の発現 …

    The Journal of Reproduction and Development Supplement 113(0), P-76-P-76, 2020

    J-STAGE 

  • Nonclinical safety assessment of trastuzumab deruxtecan (T-DXd; DS-8201), a HER2 targeting antibody-drug conjugate  [in Japanese]

    KUMAGAI Kazuyoshi

    … Before clinical trials, 6-week toxicity studies (every 3 weeks totaling 3 doses) with T-DXd were conducted in cynomolgus monkeys (cross-reactive species) and in rats (non cross-reactive species). …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), S8-4, 2020

    J-STAGE 

  • Case studies of target-mediated toxicity in antibody drug discovery  [in Japanese]

    AKAI Sho

    <p>抗体医薬品の非臨床安全性評価は,その標的分子に対する特異的な結合様式の特徴から,標的を介した毒性評価が重要である。これまでの抗体医薬品開発の多くでは,交差反応性を確認した後にカニクイザルがin vivo非臨床安全性試験に用いられてきたが,抗体反応には種差が存在することが多い。したがって,ヒトでの副作用リスクを考察するにはin vivo非臨床安全性試験だけでなく,細胞等を用いたin …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), W2-3, 2020

    J-STAGE 

  • Development of safety assessment system using primate model for practical use of genetically modified T cells  [in Japanese]

    MOCHIZUKI Hidemi , SAKAMOTO Kengo , MOROKAWA Hirokazu , TANAKA Miyuki , SAITO Shoji , YAGYU Shigeki , NAKAZAWA Yozo , SHIMOI Akihito

    <p>[諸言]世界初のCAR-T細胞薬が国内でも承認され,様々な腫瘍を標的とする新しいCAR-T細胞薬の開発が期待されている.医薬品と同様にCAR-T細胞薬も臨床試験前に安全性が担保される必要があるが,現在,広く利用されている免役不全マウスへのヒトCAR-T細胞の投与モデルでは,薬効・薬理評価は可能であっても,毒性の評価までは困難である.そこで我々は,1) ドナーとレシピエントの種が一 …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-2E, 2020

    J-STAGE 

  • Results and challenges in subretinal injection in rats, rabbits, and cynomolgus monkeys  [in Japanese]

    SAKATA Megumi , KABAYAMA Koji , ARAKI Tomoaki , TSUSAKI Hideshi

    <p>近年、加齢黄斑変性や網膜色素変性といった失明に至る疾患に対し細胞治療や遺伝子治療への取り組みが活発であり、治療手段として、アデノ随伴ウィルスやレンチウィルスベクターなどで細胞へ遺伝子導入する研究が盛んに行われている。その投与手段の一つとして着目されているのが網膜下投与である。光干渉断層計(OCT)を用いることで投与部位に形成されたブレブの詳細を経過観察することが可能であり、予後の …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-48E, 2020

    J-STAGE 

  • Placental transfer of <sup>125</sup>iodinated humanized immunoglobulin G2Δa in the cynomolgus monkey  [in Japanese]

    ISOBE Yuji , CATLIN Natasha R. , O'HARA Denise M. , WANG Mengmeng , ZHANG Minlei , BOWMAN Christopher J.

    <p>【目的】抗体医薬はFcRn受容体等を介した輸送経路により胎盤を通過し,母体から胎児に移行することが知られている。一方,いずれの動物種においても器官形成期における抗体医薬の胎盤通過性に関する報告は少ない。抗体医薬の胎盤通過性を理解することは,ヒトを含めた各動物種における発生毒性のリスク評価に有用である。</p><p>【方法】これまでに報告されている,ヒト化I …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-177, 2020

    J-STAGE 

  • Comparison and utility of animal species for convulsion risk assessment  [in Japanese]

    SHIOTANI Motohiro , YOSHINAGA Takashi , ASAKURA Shoji , SEKI Yuki , TAKANO Misato , ISHIHARA Hiroki , MAEDA Sanae , TANIGUCHI Tomohiko , MIKAMOTO Masaki , NOZAKI Yoshitane , MIYAMOTO Norimasa

    … Plasma concentrations just after convulsion onset or completion of administration were measured by LC-MS/MS. Monkeys (cynomolgus) were dosed intravenously with a limited number of the reference compounds.</p><p>[Results and discussion] Convulsion was observed in mice, rats, and monkeys with the reference compounds, except for a GAT1 competitive inhibitor which did not induce convulsion in rats. …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-137, 2020

    J-STAGE 

  • Ultrasonographic measurement of the renal resistive index in the cynomolgus monkey (Macaca fascicularis) under conscious and ketamine-immobilized conditions  [in Japanese]

    KONNO Hiroya

    … To establish a practical RRI measurement procedure in cynomolgus monkeys (Macaca fascicularis) which utilized in conventional toxicology studies, RRI was measured by ultrasonography in the spine position in conscious and ketamine-immobilized monkeys. …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-146, 2020

    J-STAGE 

  • Evaluation of autonomic nervous function and QT prolongation using Heart rate variability (HRV) in cynomolgus monkey  [in Japanese]

    SAITO Hiroyuki , KAKOI Tadashi , YAMADA Chihiro , HOSHIAI Kiyotaka , AKIE Yasuki

    <p>【背景・目的】自律神経は、交感神経系と副交感神経系で構成され、心血管系とは相補的な関係で循環を調節しており、不整脈及びQT間隔は、これらの影響を受けている。本研究では、テレメトリー法で取得した心電図データを用いて心拍変動(HRV)解析を実施し,心電図パラメーターとHRVパラメーターの関係を検討した。また、QT間隔の延長とHRVパラメーターとの関係を確認するために薬物を投与し、その …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-161, 2020

    J-STAGE 

  • Relationship between body weight and hematological and biochemical parameters in cynomolgus monkeys  [in Japanese]

    TAKAHASHI Kazuaki , SATO Junko , YAMAMOTO Dai , HIKE Norio , TAKAKI Tatsumi , ISHII Hiroyuki , OKAMURA Takayuki , OTAKE Seiji

    <p>【背景・目的】</p><p>カニクイザルを用いた毒性試験において、体重、血液学的及び血液生化学的検査の項目間の関連性は経験的に知られているものはあるが、その報告例は少ない。我々は以前、対照群ながら体重減少が認められる個体の発現率や、それら個体の血液学的、血液生化学的及び病理組織学的特徴を報告した(第46回日本毒性学会学術年会)。本研究ではそれら個体に加え、 …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-172, 2020

    J-STAGE 

  • Echocardiographic evaluation of cardiac function in cynomolgus monkeys over a wide age range

    Nakayama Shunya , Koie Hiroshi , Pai Chungyu , Ito-Fujishiro Yasuyo , Kanayama Kiichi , Sankai Tadashi , Yasutomi Yasuhiro , Ageyama Naohide

    … This report describes the first comparison of echocardiographic reference values in 247 normal cynomolgus monkeys (135 females, 112 males) over a wide age range. … These findings appear sufficiently similar to those in humans to suggest that cynomolgus monkeys can serve as a suitable model of human cardiac disease. …

    Experimental Animals 69(3), 336-344, 2020

    J-STAGE  Ichushi Web 

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