Search Results 1-20 of 451

  • Identification of effector candidate genes of Rhizoctonia solani AG-1 IA expressed during infection in Brachypodium distachyon

    Abdelsalam Sobhy S. H. , Kouzai Yusuke , Watanabe Megumi , Inoue Komaki , Matsui Hidenori , Yamamoto Mikihiro , Ichinose Yuki , Toyoda Kazuhiro , Tsuge Seiji , Mochida Keiichi , Noutoshi Yoshiteru

    … solani may undergo biotrophic process using effector proteins to suppress host immunity before necrotrophic stage. … solani secretory effector-like protein genes (RsSEPGs) were identified using in silico approach with the publicly available gene annotation of R. …

    Scientific Reports 10(1), 14889, 2020-09-10


  • Development of a Breeding Technique for Selecting Disease Resistant Soybeans Based on Non-host Resistance  [in Japanese]

    中野 真人 , 向原 隆文

    大豆たん白質研究 22(40), 126-129, 2020-06

  • Lotus Accessions Possess Multiple Checkpoints Triggered by Different Type Ⅲ Secretion System Effectors of the Wide-Host-Range Symbiont Bradyrhizobium elkanii USDA61


    Microbes and environments 35(1), 1/16-16/16, 2020-03

  • The Indian cobra reference genome and transcriptome enables comprehensive identification of venom toxins

    Kushal Suryamohan , Sajesh P. Krishnankutty , Joseph Guillory , Matthew Jevit , Markus S. Schröder , Meng Wu , Boney Kuriakose , Oommen K. Mathew , Rajadurai C. Perumal , Ivan Koludarov , Leonard D. Goldstein , Kate Senger , Mandumpala Davis Dixon , Dinesh Velayutham , Derek Vargas , Subhra Chaudhuri , Megha Muraleedharan , Ridhi Goel , Ying-Jiun J. Chen , Aakrosh Ratan , Peter Liu , Brendan Faherty , Guillermo de la Rosa , Hiroki Shibata , Miriam Baca , Meredith Sagolla , James Ziai , Gus A. Wright , Domagoj Vucic , Sangeetha Mohan , Aju Antony , Jeremy Stinson , Donald S. Kirkpatrick , Rami N. Hannoush , Steffen Durinck , Zora Modrusan , Eric W. Stawiski , Kristen Wiley , Terje Raudsepp , R. Manjunatha Kini , Arun Zachariah , Somasekar Seshagiri

    … Of the 23,248 predicted protein-coding genes, 12,346 venom-gland-expressed genes constitute the 'venom-ome' and this included 139 genes from 33 toxin families. … Among the 139 toxin genes were 19 'venom-ome-specific toxins' (VSTs) that showed venom-gland-specific expression, and these probably encode the minimal core venom effector proteins. …

    Nature Genetics 52(1), 106-117, 2020-01-06


  • The <i>in vitro</i> and <i>in vivo</i> anti-virulence activities of <i>Cinnamomum bejolghota</i> by inhibiting type three secretion system effector proteins of Salmonella

    Liu Yan , Zhang Dongdong , Gao Rongrong , Zhang Xiaochun , Yang Xuefei , Lu Chunhua

    … significantly inhibited secretion effector proteins of the type three secretion system (T3SS) in Salmonella. … inhibited the secretion of effector proteins SipA, SipB, SipC and SipD without affecting bacteria growth and the translocation of SipC into MDA-MB-231 cells. …

    Drug Discoveries & Therapeutics 14(5), 243-248, 2020


  • Silencing of phosphoinositide dependent protein kinase orthologs reduces hypersensitive cell death in <i>Nicotiana benthamiana</i>

    Kiba Akinori , Fukui Kotoko , Mitani Maki , Galis Ivan , Hojo Yuko , Shinya Tomonori , Ohnishi Kouhei , Hikichi Yasufumi

    … Here we focused on phosphoinositide dependent protein kinases (PDKs) as a candidate required for phosphatidic acid signaling. …

    Plant Biotechnology 37(3), 363-367, 2020


  • Current issues on reflection paper for genome editing gene therapy  [in Japanese]

    YAMAGUCHI Teruhide

    <p>目的とする遺伝子を特異的に切断、改変、編集できる画期的な技術としてゲノム編集技術の開発が精力的に進められており、新たな遺伝子治療法として、その実用化が期待されている。ゲノム編集は、ゲノム配列特異的に目的遺伝子の機能を失わせたり、疾患の原因となっている遺伝子異常を修正したりすることが可能な技術として究極の遺伝子治療技術となると期待されている。</p><p>ゲ …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), S15-1, 2020


  • Gomisin N Exerts Anti-liver Cancer Effects and Regulates PI3K–Akt and mTOR–ULK1 Pathways <i>in Vitro</i>

    Zhu Pei-Li , Wu Jia-Ying , Wu Ying , Bai Jing-Xuan , Liang Chun , Yu Zhi-Ling , Lam Dick Fai , Li Jun-Kui , Fu Xiu-Qiong , Yin Cheng-Le , Chou Ji-Yao , Wang Ya-Ping , Liu Yu-Xi , Chen Ying-Jie

    … Mechanistic studies revealed that GN lowers protein levels of phospho-PI3K (p85 tyrosine (Tyr)458), phospho-Akt (serine (Ser)473), and Akt downstream molecules Mcl-1 in HepG2 and HCCLM3 cells. … Meanwhile, GN activates mTOR and inhibits ULK1 (a negative downstream effector of mTOR) activities. …

    Biological and Pharmaceutical Bulletin 43(8), 1267-1271, 2020


  • Genome Engineering Evolves Brain Tumor Modeling

    KOGA Tomoyuki , CHEN Clark C. , FURNARI Frank B.

    … <p>Genome engineering using programmable nucleases such as transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeat-associated protein nine facilitated the introduction of genetic alterations at specific genomic sites in various cell types. …

    Neurologia medico-chirurgica 60(7), 329-336, 2020


  • Type III Secretion System of <i>Bradyrhizobium</i> sp. SUTN9-2 Obstructs Symbiosis with <i>Lotus</i> spp.

    Hashimoto Shun , Goto Kohki , Pyromyou Pongdet , Songwattana Pongpan , Greetatorn Teerana , Tittabutr Panlada , Boonkerd Nantakorn , Teaumroong Neung , Uchiumi Toshiki

    … <p>The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. … A mutant of SUTN9-2 that is unable to secrete effector proteins showed better nodulation and plant growth promotion than wild-type SUTN9-2 when paired with these <i>Lotus</i> …

    Microbes and Environments 35(3), n/a, 2020


  • Targeted genome editing in tetraploid potato through transient TALEN expression by <i>Agrobacterium</i> infection

    Yasumoto Shuhei , Sawai Satoru , Lee Hyoung Jae , Mizutani Masaharu , Saito Kazuki , Umemoto Naoyuki , Muranaka Toshiya

    … <p>Genome editing using site-specific nucleases, such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat–CRISPR-associated protein 9 (CRISPR-Cas9), is a powerful technology for crop breeding. …

    Plant Biotechnology 37(2), 205-211, 2020


  • Shedding light on complexity of G-protein coupling by large-scale functional assays  [in Japanese]

    Inoue Asuka

    … G-protein-coupling pattern determines a cellular response unique to each GPCR and thus profiling these patterns is a critical step toward understanding GPCR biology. … However, due to functional redundancy of multiple members and signal cross talk downstream of G-protein signaling, it is challenging to assess coupling signature of every G-protein member at a large scale. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-S28-2, 2020


  • Ca<sup>2+</sup>-activated K<sup>+</sup> channel K<sub>Ca</sub>2.2 inhibiter as a possible therapy for advanced prostate cancer  [in Japanese]

    Niwa Satomi , Tanaka Satoshi , Ohya Susumu

    … The pharmacological or siRNA-mediated inhibition of AR for 48 hr decreased the expression level levels of K<sub>Ca</sub>2.2 transcripts in LNCaP cells, whereas the UCL1684-induced inhibition of K<sub>Ca</sub>2.2 activity did not affect the expression levels of AR transcripts, suggesting that K<sub>Ca</sub>2.2 is a downstream effector of AR signaling in LNCaP cells. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-O-089, 2020


  • <i>Lotus</i> Accessions Possess Multiple Checkpoints Triggered by Different Type III Secretion System Effectors of the Wide-Host-Range Symbiont <i>Bradyrhizobium elkanii</i> USDA61

    Kusakabe Shohei , Higasitani Nahoko , Kaneko Takakazu , Yasuda Michiko , Miwa Hiroki , Okazaki Shin , Saeki Kazuhiko , Higashitani Atsushi , Sato Shusei

    … By conducting inoculation tests with mutants of previously reported and newly identified effector protein genes of <i>B. … USDA61, we identified NopF as the effector protein triggering the inhibition of infection, and NopM as the effector protein triggering the nodule early senescence–like response. …

    Microbes and Environments 35(1), n/a, 2020


  • Pathological characteristics of <i>Ccdc85c</i> knockout rats: a rat model of genetic hydrocephalus

    Konishi Shizuka , Kuwamura Mitsuru , Tanaka Natsuki , Mashimo Tomoji , Yamamoto Takashi , Sakuma Tetsushi , Kaneko Takehito , Tanaka Miyuu , Izawa Takeshi , Yamate Jyoji

    … KO rats were produced by genomic engineering using transcription activator-like effector nuclease (TALEN). … and lacked CCDC85C protein expression. …

    Experimental Animals 69(1), 26-33, 2020


  • Fat-specific protein 27b is regulated by hepatic peroxisome proliferator-activated receptor γ in hepatic steatosis

    Aibara Daisuke , Matsuo Kohei , Yamano Shigeru , Matsusue Kimihiko

    … <p>The fat-specific protein 27 gene (<i>Fsp27</i>) belongs to the cell death-inducing DNA fragmentation factor 45-like effector family. …

    Endocrine Journal 67(1), 37-44, 2020


  • POGLUT1, the putative effector gene driven by rs2293370 in primary biliary cholangitis susceptibility locus chromosome 3q13.33

    Hitomi Yuki , Ueno Kazuko , Kawai Yosuke , Nishida Nao , Kojima Kaname , Kawashima Minae , Aiba Yoshihiro , Nakamura Hitomi , Kouno Hiroshi , Kouno Hirotaka , Ohta Hajime , Sugi Kazuhiro , Nikami Toshiki , Yamashita Tsutomu , Katsushima Shinji , Komeda Toshiki , Ario Keisuke , Naganuma Atsushi , Shimada Masaaki , Hirashima Noboru , Yoshizawa Kaname , Makita Fujio , Furuta Kiyoshi , Kikuchi Masahiro , Naeshiro Noriaki , Takahashi Hironao , Mano Yutaka , Yamashita Haruhiro , Matsushita Kouki , Tsunematsu Seiji , Yabuuchi Iwao , Nishimura Hideo , Shimada Yusuke , Yamauchi Kazuhiko , Komatsu Tatsuji , Sugimoto Rie , Sakai Hironori , Mita Eiji , Koda Masaharu , Nakamura Yoko , Kamitsukasa Hiroshi , Sato Takeaki , Nakamuta Makoto , Masaki Naohiko , Takikawa Hajime , Tanaka Atsushi , Ohira Hiromasa , Zeniya Mikio , Abe Masanori , Kaneko Shuichi , Honda Masao , Arai Kuniaki , Arinaga-Hino Teruko , Hashimoto Etsuko , Taniai Makiko , Umemura Takeji , Joshita Satoru , Nakao Kazuhiko , Ichikawa Tatsuki , Shibata Hidetaka , Takaki Akinobu , Yamagiwa Satoshi , Seike Masataka , Sakisaka Shotaro , Takeyama Yasuaki , Harada Masaru , Senju Michio , Yokosuka Osamu , Kanda Tatsuo , Ueno Yoshiyuki , Ebinuma Hirotoshi , Himoto Takashi , Murata Kazumoto , Shimoda Shinji , Nagaoka Shinya , Abiru Seigo , Komori Atsumasa , Migita Kiyoshi , Ito Masahiro , Yatsuhashi Hiroshi , Maehara Yoshihiko , Uemoto Shinji , Kokudo Norihiro , Nagasaki Masao , Tokunaga Katsushi , Nakamura Minoru

    … Moreover, e-QTL analysis indicated that the effector gene of rs2293370 was Protein O-Glucosyltransferase 1 (POGLUT1) (P = 3.4 × 10⁻⁸). … This is the first study to demonstrate that POGLUT1 and not CD80 is the effector gene regulated by the primary functional SNP rs2293370, and that increased expression of POGLUT1 might be involved in the pathogenesis of PBC. …

    Scientific reports (9), 2019-01-14


  • Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from alveolar epithelial cells

    Umezawa Kanoko , Nagano Tatsuya , Kobayashi Kazuyuki , Dokuni Ryota , Katsurada Masahiro , Yamamoto Masatsugu , Yoshikawa Yoko , Kataoka Tohru , Nishimura Yoshihiro

    … Background We have shown that phospholipase Cε (PLCε), an effector of Ras and Rap1 small GTPases, plays pivotal roles in inflammation and inflammation-associated carcinogenesis by augmenting proinflammatory cytokine production from epithelial cells of various organs. … Primary cultures of AECs, established from PLCε+/+ and PLCεΔX/ΔX mice, are used to analyze the roles of PLCε, protein kinase D (PKD) and nuclear factor-κB (NF-κB) in augmentation of LPS-induced Cxcl5 expression. …

    Respiratory Research (20), 9, 2019-01-11


  • A short LysM protein with high molecular diversity from an arbuscular mycorrhizal fungus, Rhizophagus irregularis

    Schmitz Alexa M. , Pawlowska Teresa E. , Harrison Maria J.

    Mycoscience : official journal of the Mycological Society of Japan 60(1), 63-70, 2019-01

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