Search Results 1-20 of 1519

  • Functional maintenance of calcium store by ShcB adaptor protein in cerebellar Purkinje cells

    Kakizawa Sho , Kishimoto Yasushi , Yamamoto Shinichiro , Onga Kazuko , Yasuda Kunihiko , Miyamoto Yoshiaki , Watanabe Masahiko , Sakai Ryuichi , Mori Nozomu

    … ntracellular Ca2+ levels are changed by influx from extracellular medium and release from intracellular stores. … In the central nervous systems, Ca2+ release is involved in various physiological events, such as neuronal excitability and transmitter release. …

    Scientific Reports 10(1), 14475, 2020-09-02

    IR 

  • Roles of Interaction between CCN2 and Rab14 in Aggrecan Production by Chondrocytes

    Hoshijima Mitsuhiro , Hattori Takako , Aoyama Eriko , Nishida Takashi , Kubota Satoshi , Kamioka Hiroshi , Takigawa Masaharu

    … To detect the colocalization between CCN2 and Rab14 in the cells in detail, CCN2, wild-type Rab14 (Rab14WT), a constitutive active form (Rab14CA), and a dominant negative form (Rab14DN) of Rab14 were overexpressed in monkey kidney-tissue derived COS7 cells. … When Rab14CA was overexpressed with CCN2, Rab14CA and CCN2 also showed good localization as dots, but their distribution was more widespread within cytosol. …

    International Journal of Molecular Sciences 21(8), 2020-04-16

    IR 

  • The Effect of Thymoquinone on the Characteristics of the Brain Extracellular Space in Transient Middle Cerebral Artery Occlusion Rats

    Fan Chaoxin , Tian Fang , Zhao Xin , Sun Yi , Yang Xiaogai , Han Hongbin , Pu Xiaoping

    … <p>The extracellular space (ECS) is the space between the neurons and the capillaries in the brain. … Also, H&E staining showed that thymoquinone could inhibit the neuron necrosis in the hippocampal CA1 region. …

    Biological and Pharmaceutical Bulletin 43(9), 1306-1314, 2020

    J-STAGE 

  • Characteristics of Hemichannel-Mediated Substrate Transport in Human Retinal Pigment Epithelial Cells under Deprivation of Extracellular Ca<sup>2+</sup>

    Maruyama Souhei , Akanuma Shin-ichi , Kubo Yoshiyuki , Hosoya Ken-ichi

    … Recent studies have elucidated that RPE cells express hemichannels, which are opened by extracellular Ca<sup>2+</sup> … In human RPE cells, namely ARPE-19 cells, time-dependent uptake of fluorescent hemichannel substrates, such as Lucifer Yellow, sulforhodamine-101 (SR-101), and propidium iodide (PI) was promoted under Ca<sup>2+</sup>-depleted conditions. …

    Biological and Pharmaceutical Bulletin 43(8), 1241-1247, 2020

    J-STAGE 

  • Zinc Increases ABCA1 by Attenuating Its Clearance Through the Modulation of Calmodulin Activity

    Lu Rui , Ishikawa Takahiro , Tanaka Mamoru , Tsuboi Tomoe , Yokoyama Shinji

    … <p><b>Aim: </b>We previously revealed that Ca<sup>++</sup>-activated calmodulin binds to ABCA1 by the region near the PEST sequence and retards its calpain-mediated degradation to increase HDL biogenesis. … Accordingly, it enhanced cell cholesterol release with extracellular apolipoprotein A-I. … and Ca<sup>++</sup>. …

    Journal of Atherosclerosis and Thrombosis, 2020

    J-STAGE 

  • Orexins modulate membrane excitability in rat trigeminal motoneurons

    Tanaka Susumu , Higuchi Masataka , Seki Soju , Enomoto Akifumi , Kogo Mikihiko

    … and Ca<sup>2+</sup> … Extracellular and intracellular Ca<sup>2+</sup> … were involved in regulating modulatory effects, but a requisite level of intracellular Ca<sup>2+</sup> …

    Journal of Oral Science 62(3), 265-270, 2020

    J-STAGE 

  • Metalloprotease nardilysin controls heart rate through the transcriptional regulation of ion channels critical for sinus automaticity.  [in Japanese]

    Ohno Mikiko , Matsuura Hiroshi , Makiyama Takeru , Kojitani Hirohiko , Nishi Kiyoto , Iwasaki Hirotaka , Matsuda Shintaro , Kimura Takeshi , Nishi Eiichiro

    … an enhancer of ectodomain shedding in the extracellular space and a transcriptional coregulator in the nucleus. … (3) Funny (If) current and T-type Ca current measured in the sinus node cells were markedly reduced in <i>Nrdc-/-</i> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-P-332, 2020

    J-STAGE 

  • Effects of thrombospondin-4 on voltage-gated ion channels in rat ventricular myocytes  [in Japanese]

    Okada Muneyoshi , Aratani Momoko , Koyama Takahiro , Imoto Keisuke , Yamawaki Hideyuki

    <p>Thrombospondin (TSP)-4, a matricellular protein, is highly expressed in heart tissues of various cardiac disease models. Although TSP-4 is known to regulate voltage-dependent calcium channel …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-P-329, 2020

    J-STAGE 

  • The mechanism of serotonin-induced increase in intracellular Ca<sup>2+</sup> and constriction via Rho kinase in rat thoracic aortas  [in Japanese]

    Terada Yuka , Kishimoto Minami , Torii Karin , Yayama Katsutoshi

    … <p>The mechanism of serotonin (5-HT)-induced vasoconstriction and intracellular Ca<sup>2+</sup> … ([Ca<sup>2+</sup>]i) mobilization is not completely elucidated. … 5-HT-induced vasoconstriction partly involves Ca<sup>2+</sup>-independent activation of Rho kinase. … We examined the mechanism of 5-HT-induced [Ca<sup>2+</sup>]i mobilization of rat aortic smooth muscle cells using microscopic fluorometry. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-O-096, 2020

    J-STAGE 

  • Periostin prolongs action potential duration thorough inhibiting voltage-dependent Na<sup>+</sup> channel activity in rat ventricular myocytes  [in Japanese]

    Imoto Keisuke , Okada Muneyoshi , Yamawaki Hideyuki

    <p>Periostin (POSTN), a matricellular protein is related to structural remodeling of pathological heart. However, it remains to be clarified whether POSTN mediates electrical disorders of heart. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-O-080, 2020

    J-STAGE 

  • Neuroimmune crosstalk in neuropathic and visceral pain: HMGB1 and ATP as key mediators  [in Japanese]

    Kawabata Atsufumi

    … The extracellular HMGB1 induces NK-κB-dependent upregulation of cystathionine-γ-lyase, an H<sub>2</sub>S-generating enzyme, in the urothelium by activating RAGE, and the generated H<sub>2</sub>S enhances the activity of Ca<sub>v</sub>3.2 T-type calcium channels expressed in nociceptors, resulting in bladder pain. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-S24-4, 2020

    J-STAGE 

  • The role of meningeal mast cells in ATP-induced nociceptive firing in trigeminal afferents. Anti-nociceptive effects of hydrogen sulfide  [in Japanese]

    Sitdikova Guzel , Koroleva Ksenia , Ermakova Elizaveta , Mustafina Alsu , Giniatullin Rashid

    Extracellular ATP released during migraine attacks activates meningeal afferents via neuronal P2X3 receptors. … Moreover, H2S decreased currents and Ca2+ responses mediated by activation of P2X3 receptors in trigeminal neurons. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-S24-2, 2020

    J-STAGE 

  • Neuronal hyperexcitability mediated by GqPCR-mediated signaling in astrocytes  [in Japanese]

    Shigetomi Eiji , Hirayama Yukiho , Sano Fumikazu , Tanaka Kenji , Bito Haruhiko , Koizumi Schuichi

    … <p>Astrocytes maintain extracellular milieu, provide nutrients and release signaling molecules to regulate neuronal excitability. … In disease, astrocytes generally become reactive and reactive astrocytes show more Ca<sup>2+</sup> … To investigate the role of augmented GqPCR-mediated Ca<sup>2+</sup> … Using dual color Ca<sup>2+</sup> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-P-143, 2020

    J-STAGE 

  • A dendritic spine shrinkage in response to extracellular high K<sup>+</sup> is inhibited by Co<sup>2+</sup> or Cd<sup>2+ </sup>  [in Japanese]

    Konishi Naoko , Sawano Toshinori , Nakatani Jin , Tanaka Hidekazu

    … An extracellular high K<sup>+</sup> … was inhibited in the presence of extracellular Co<sup>2+</sup> … Then we investigated Ca<sup>2+</sup>-signaling pathway because these metal ions are supposed to be wide spectrum Ca<sup>2+</sup>-channel blockers. … Taken together, extracellular high K+-triggered Ca<sup>2+</sup>-influx seem to induce the spine shrinkage; …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-P-139, 2020

    J-STAGE 

  • Cancer cells co-opt the neuronal redox-sensing channel TRPA1 to promote oxidative-stress tolerance  [in Japanese]

    Nobuaki Takahashi

    … Cancer cells are subjected to numerous cellular insults, including dysregulated oncogenes and dissociation from their natural extracellular matrix niches, leading to the generation of high levels of ROS; … Here, we show a non-canonical oxidative-stress defense mechanism through TRPA1, the redox-sensing Ca<sup>2+</sup>-influx channel that we previously found in sensory and vagal neurons (Takahashi et al., <i>Nature Chem. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-YIA-14, 2020

    J-STAGE 

  • Elucidation of neurite outgrowth by Wnt5a Ca<sup>2 +</sup>-dependently produced in periodontal ligament cells  [in Japanese]

    Takahashi Kaori , Yoshida Takashi , Wakamori Minoru

    … The depletion of extracellular Ca<sup>2+</sup> … These results suggest that the mechanically stimulated PDL cells produce Wnt5a in a Ca<sup>2+</sup> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-SS-40, 2020

    J-STAGE 

  • Novel defense strategy against progressive neurodegeneration by controlling intracellular Zn<sup>2+ </sup>dysregulation  [in Japanese]

    Takeda Atsushi

    … <p>The causes of progressive neurodegeneration, i.e., Alzheimer's disease (AD) and Parkinson's disease (PD) are unknown. The basal (static) concentration of intracellular Zn<sup>2+ </sup>is estimated to be approximately 100 pM and is extremely lower than that of intracellular Ca<sup>2+ </sup>(~100 nM), suggesting that intracellular Zn<sup>2+ </sup>homeostasis is crucial for neural function. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-S17-3, 2020

    J-STAGE 

  • Involvement of Redox Modification of Ca<sup>2+</sup>-Release Channels in Cerebellar Learning and Aging  [in Japanese]

    Kakizawa Sho

    … <p>Ca<sup>2+</sup> … release from intracellular store, as well as Ca<sup>2+</sup> … influx from extracellular fluid, contributes elevation of cytosolic Ca<sup>2+</sup> … However, regulatory mechanisms of Ca<sup>2+</sup>-release channels and their functional roles in relation to learning and memory have not been fully understood. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-S07-4, 2020

    J-STAGE 

  • Projection-identified large-scale recording reveals pathway-specific information outflow from the subiculum  [in Japanese]

    Kitanishi Takuma

    … The subiculum is the major hippocampal output structure that receives the hippocampal CA1 output and projects to multiple cortical/subcortical areas. … We investigated this issue by optogenetically identifying the projection targets of subicular neurons during large-scale extracellular recordings in freely behaving rats. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-S08-1, 2020

    J-STAGE 

  • Imaging of extracellular Ca<sup>2+</sup> in the hippocampus by a novel CMOS ion image sensor  [in Japanese]

    Parajuli Bijay , Doi Hideo , Shigetomi Eiji , Shinozaki Youichi , Lee Youna , Noda Toshihiko , Takahashi Kazuhiro , Sawada Kazuaki , Koizumi Schuichi

    … <p>Intracellular calcium ion (Ca<sup>2+</sup>i) is one of the most important cation that controls several cellular functions, and thus there were already huge number of literature about Ca<sup>2+</sup>i imaging in various cells. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-P-126, 2020

    J-STAGE 

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