Search Results 1-20 of 1574

  • Development of drug delivery system (DDS) for gene and nucleic acid from the clinical side  [in Japanese]

    兒玉 幸修 , 黒崎 友亮 , 佐々木 均

    月刊薬事 = The pharmaceuticals monthly 62(5), 1027-1030, 2020-04

  • Effects of Tissue Pressure on Transgene Expression Characteristics via Renal Local Administration Routes from Ureter or Renal Artery in the Rat Kidney

    Oyama Natsuko , Takahashi Haruyuki , Kawaguchi Maho , Miyamoto Hirotaka , Nishida Koyo , Tsurumaru Masako , Nakashima Mikiro , Yamashita Fumiyoshi , Hashida Mitsuru , Kawakami Shigeru

    … We previously developed a renal pressure-mediated transfection method (renal pressure method) as a kidney-specific in vivo gene delivery system. … In this study, we selected renal arterial and ureteral injections as local administration routes and evaluated the characteristics of gene delivery such as efficacy, safety, and distribution in pressured kidney of rat. …

    Pharmaceutics 12(2), 114, 2020-02-01

    IR 

  • Effects of Tissue Pressure on Transgene Expression Characteristics via Renal Local Administration Routes from Ureter or Renal Artery in the Rat Kidney

    Oyama Natsuko , Takahashi Haruyuki , Kawaguchi Maho , Miyamoto Hirotaka , Nishida Koyo , Tsurumaru Masako , Nakashima Mikiro , Yamashita Fumiyoshi , Hashida Mitsuru , Kawakami Shigeru

    … We previously developed a renal pressure-mediated transfection method (renal pressure method) as a kidney-specific in vivo gene delivery system. … In this study, we selected renal arterial and ureteral injections as local administration routes and evaluated the characteristics of gene delivery such as efficacy, safety, and distribution in pressured kidney of rat. …

    Pharmaceutics 12(2), 2020-02

    IR 

  • Isolation of a Novel Bacterial Strain Capable of Producing Abundant Extracellular Membrane Vesicles Carrying a Single Major Cargo Protein and Analysis of Its Transport Mechanism

    Chen Chen , Kawamoto Jun , Kawai Soichiro , Tame Akihiro , Kato Chiaki , Imai Tomoya , Kurihara Tatsuo

    … EMVs have potential for use as vaccines, drug-delivery vehicles, platforms for extracellular production of recombinant proteins, and so on. … Disruption of the P49 gene resulted in only a marginal decrease in the EMV production, indicating that the EMVs are produced even in the absence of the major cargo protein. …

    Frontiers in microbiology (10), 2020-01-14

    IR 

  • Modification of Mannosylerythritol Lipids (MELs) Producing Yeast by Genetic Engineering  [in Japanese]

    SAIKA Azusa

    <p>マンノシルエリスリトールリピッド(MEL)は糖型バイオサーファクタントのひとつであり,そのユニークで多彩な機能から,界面活性剤としての利用だけでなく,環境適合型の機能性バイオ化学品として注目を集めている。現在,MELは化粧品・日用品分野で実用化されているが,今後のさらなる用途拡大,また医薬,農業,食品等の新規分野への参入には,生産性の向上と構造・機能の拡充が大きな課題となっている …

    Oleoscience 20(5), 187-193, 2020

    J-STAGE 

  • Effect of Hydrophilic Chain Length in Non-Ionic Surfactant on the Physicochemical Characteristics, Gene Expression Efficiency and Cytotoxicity of DNA/Poly-L-Ornithine/Niosome Ternary Complexes for Gene Delivery

    Kurihara Jun , Uchida Masaki , Yamaki Tsutomu , Hatanaka Tomomi , Seki Toshinobu , Natsume Hideshi

    … <p>The aim of this study is to clarify the effect of hydrophilic ethylene oxide (EO) chain length in steareths on the physicochemical characteristics, gene expression efficiency and cytotoxicity of plasmid DNA (pDNA)/poly-L-ornithine (PLO)/niosomes (consisting of non-ionic surfactants [NISs], polyoxyethylene stearyl ethers [steareths]) ternary complexes. …

    Journal of Pharmaceutical Science and Technology, Japan 80(3), 156-161, 2020

    J-STAGE 

  • Development of Novel Drug Delivery System Targeting Exosomal microRNA  [in Japanese]

    Yamayoshi Asako

    … These exosomal-miRNAs may regulate gene expression in recipient cells. … miRNAs are a type of non-coding RNA that induce post-transcriptional gene silencing of their target genes and regulate a wide range of biological processes, including apoptosis, differentiation, metabolism, and cell proliferation. … In this study, we developed novel drug delivery system using anti-exosome antibody-oligonucleotide conjugates (ExomiR-Tracker) for functional inhibition of circulating miRNAs. …

    YAKUGAKU ZASSHI 140(5), 625-631, 2020

    J-STAGE 

  • Introduction of Chimeric Antigen Receptor T-cell therapy, Tisagenlecleucel in Japan  [in Japanese]

    Fujiwara Yuki , Park Jihyun , Dochi Takeo , Tsurumaki Yoshie

    2019年3月、日本で初めて免疫細胞療法であるキメラ抗原受容体T細胞療法(chimeric antigen receptor-T cell therapy:CAR-T細胞療法)、チサゲンレクルユーセル(製品名;キムリア<sup>®</sup>点滴静注、ノバルティス)が承認された。チサゲンレクルユーセルの適応は、再発または難治性のCD19陽性のB細胞性急性リンパ芽球性白血病( …

    Drug Delivery System 35(1), 71-75, 2020

    J-STAGE 

  • NADH peroxidase plays a crucial role in consuming H<sub>2</sub>O<sub>2</sub> in <i>Lactobacillus casei</i> IGM394

    NARAKI Shingo , IGIMI Shizunobu , SASAKI Yasuko

    … IGM394 is used as a host for drug delivery systems, and it exhibits the same growth rate under aerobic and anaerobic conditions. … accumulation were observed in the NADH peroxidase gene-mutated strain (Δ<i>npr</i>) compared with the findings in the wild type. … IGM394 has multiple oxygen tolerance mechanisms and that the loss of a single gene does not alter the growth rate because of the presence of complementary mechanisms. …

    Bioscience of Microbiota, Food and Health 39(2), 45-56, 2020

    J-STAGE 

  • Lipid Nanoparticles for Cell-Specific <i>in Vivo</i> Targeted Delivery of Nucleic Acids

    Khalil Ikramy A. , Younis Mahmoud A. , Kimura Seigo , Harashima Hideyoshi

    … targeted delivery of nucleic acids. … Compared with other systems used for nonviral gene delivery, LNPs provide several advantages including higher stability, low toxicity, and greater efficiency. … Efforts are ongoing to improve the efficiency of lipid-based gene vectors. … These efforts depend on the appropriate design of nanocarriers as well as the development of new lipids with improved gene delivery ability. …

    Biological and Pharmaceutical Bulletin 43(4), 584-595, 2020

    J-STAGE 

  • Therapeutic Arteriogenesis/Angiogenesis for Peripheral Arterial Disease by Nanoparticle-Mediated Delivery of Pitavastatin into Vascular Endothelial Cells

    Matsumoto Takuya , Egashira Kensuke , Yamashita Sho , Yoshino Shinichiro , Kurose Shun , Morisaki Koichi , Nakano Kaku , Koga Jun-ichiro , Furuyama Tadashi , Mori Masaki

    … When therapeutic angiogenesis first began, local administration was conducted using recombinant growth factor proteins or gene-encoding growth factors for endothelial cells. … We have developed a nanoparticle (NP)-mediated, drug-targeting delivery system using bioabsorbable poly-lactic/glycolic acid (PLGA) NPs. …

    Annals of Vascular Diseases 13(1), 4-12, 2020

    J-STAGE 

  • Pathological mechanisms and novel therapeutic outlooks for sepsis-associated acute lung injury  [in Japanese]

    Hattori Yuichi , Hattori Kohshi , Matsuda Naoyuki

    … A number of transcription factors, such as nuclear factor-kappaB and activator protein-1, can be linked to the altered gene activation during sepsis. … Given that sepsis can be regarded as a gene-related disorder, the potential usefulness of systemic delivery of decoy oligodeoxynucleotide against some transcription factors may be considered to be a promising novel therapeutic approach for treatment of acute lung injury in sepsis.</p> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-S34-3, 2020

    J-STAGE 

  • Application for drug discovery using mammalian artificial chromosomes  [in Japanese]

    Kazuki Yasuhiro

    … HAC/MAC as gene delivery vectors can deliver Mb-sized gene cluster and multiple genes, and are stably and independently maintained with defined copy numbers in host cells, as well as being transferrable to any other cell line via microcell-mediated chromosome transfer (MMCT). We have demonstrated several applications for drug discovery using the HAC/MAC, including 1)Humanized mice/rats and multifunctional model cells for predicting human drug metabolism, 2) Novel Down syndrome model animals and …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-S19-2, 2020

    J-STAGE 

  • Nose-to-brain delivery of CGRP against fear memory retention in mice  [in Japanese]

    Hashikawa-Hobara Narumi , Andou Hiroki , Morimoto Himika , Hashikawa Naoya

    … <p>We previously reported that intracerebroventricular administration of calcitonin gene-related peptide (CGRP) effect on hippocampus-dependent fear memory in mice. … The intranasal route offers an alternative approach for drug delivery to the brain without the interference of the blood-brain-barrier. … In this study, we evaluated the nose-to-brain delivery of CGRP to investigate the effects on fear memory retention by contextual learning test. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-P-034, 2020

    J-STAGE 

  • Development of Vaccines with Self-assembled Carriers That Deliver Drugs to Target Organs  [in Japanese]

    Kurosaki Tomoaki

    … After the intravenous administration of γ-PGA-ternary complexes to mice, extremely high gene expression was detected in the marginal zone of the spleen, which is rich in antigen-presenting cells. …

    YAKUGAKU ZASSHI 140(3), 363-368, 2020

    IR  J-STAGE 

  • Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation

    YAMASHITA Shiro , KOGASAKA Yuhei , HIRADATE Yuuki , TANEMURA Kentaro , SENDAI Yutaka

    Gene-modified animals, including pigs, can be generated efficiently by introducing CRISPR associated protein 9 (CRISPR/Cas9) into zygotes. … However, in many cases, these zygotes tend to become mosaic mutants with various different mutant cell types, making it difficult to analyze the phenotype of gene-modified founder animals. …

    Journal of Reproduction and Development 66(1), 41-48, 2020

    J-STAGE 

  • Development of an RNA Virus-Based Episomal Vector Capable of Switching Transgene Expression

    Yamamoto Yusuke , Tomonaga Keizo , Honda Tomoyuki

    … Viral vectors are efficient gene delivery systems, although most of these vectors still present limitations to their practical use, such as achieving only transient transgene expression and a risk of insertional mutations. … To our knowledge, REVec-L2b9 is the first nuclear-replicating RNA virus vector capable of switching transgene expression on and off as needed, which will expand the potential for gene therapies by increasing safety and usability. …

    Frontiers in Microbiology (10), 2019-11-06

    IR 

  • Modified nucleobase-specific gene regulation using engineered transcription activator-like effectors

    Tsuji Shogo , Imanishi Miki

    … Epigenetic modification, as typified by cytosine methylation, is a key aspect of gene regulation that affects many biological processes. …

    Advanced Drug Delivery Reviews (147), 59-65, 2019-07

    IR 

  • Detection of Transgenes in Gene Delivery Model Mice by Adenoviral Vector Using ddPCR

    川上 康 , Takehito Sugasawa , Kai Aoki , Koichi Watanabe , Koki Yanazawa , Tohru Natsume , Tohru Takemasa , Kaori Yamaguchi , Yoshinori Takeuchi , Yuichi Aita , Naoya Yahagi , Yasuko Yoshida , Katsuyuki Tokinoya , Nanami Sekine , Kaoru Takeuchi , Haruna Ueda , Yasushi KAWAKAMI , Satoshi Shimizu , Kazuhiro Takekoshi

    … With the rapid progress of genetic engineering and gene therapy, the World Anti-Doping Agency has been alerted to gene doping and prohibited its use in sports. … Here, we aim to develop a detection method for transgenes delivered by rAdV vectors in a mouse model that mimics gene doping. … These rAdV vectors containing the mCherry gene was delivered in mice through intravenous injection or local muscular injection. …

    Genes 10(6), 436, 2019-06

    IR 

  • <一般演題抄録>Ultrasound-Targeted Gene Delivery Induces Angiogenesis After a Myocardial Infarction in Mice

    Fujii Hiroko , Sun Zhuo , Li Shu-Hong , Wu Jun , Fazel Shafie , Weisel Richard D , Rakowski Harry , Lindner Jonathan , Li Ren-Ke

    弘前医学 69(1-4), 201-201, 2019-03-15

    IR 

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