Search Results 1-20 of 308

  • Effects of High-intensity Interval Training on Lactate Dehydrogenase Expression in Skeletal Muscle Fibers  [in Japanese]

    西口 治佳 , 小山 健藏 , 榎木 泰介

    競技スポーツでは,パフォーマンスの向上を目的として様々な形態のトレーニングが行われている。その中でも近年,高強度インターバルトレーニングが注目されている。高強度運動は速筋線維群を動員する割合が高く,解糖系代謝を刺激するなどの特性がある。高強度運動を短い休息を挟んで反復する高強度インターバルトレーニングは,解糖系代謝による乳酸の産生だけではなく,休息時には乳酸の分解(酸化代謝)が活性化する効果も想定 …

    大阪教育大学紀要. 人文社会科学・自然科学 = Memoirs of Osaka Kyoiku University. Humanities and social science, natural science 69, 1-7, 2021-02

    IR  DOI 

  • Study on regulation of skeletal muscle characteristics by food components  [in Japanese]

    KOMIYA Yusuke

    <p>骨格筋を構成する筋線維は異なる性質を示す遅筋タイプと速筋タイプに大別される.遅筋タイプはミトコンドリアに富み,酸化能力に長け,抗疲労性を示す.食肉においては遅筋タイプの割合が高まると,テクスチャー改善,機能性成分の増加,呈味性向上などにつながる.これまで後天的な遅筋タイプの増加は運動トレーニングによってのみ生じると考えられていた.我々はオレイン酸を骨格筋細胞に作用させると遅筋タイ …

    Nihon Chikusan Gakkaiho 92(1), 25-33, 2021

    J-STAGE 

  • Dysfunction of CD8+PD-1+T cells in type 2 diabetes caused by the impairment of metabolism-immune axis

    Nojima Ichiro , Eikawa Shingo , Tomonobu Nahoko , Hada Yoshiko , Kajitani Nobuo , Teshigawara Sanae , Miyamoto Satoshi , Tone Atsuhito , Uchida Haruhito A. , Nakatsuka Atsuko , Eguchi Jun , Shikata Kenichi , Udono Heiichiro , Wada Jun

    … The disturbance of the link between metabolism and immune function in CD8+PD-1+T cells in T2D was proved by recovery of antigen-specific and non-specific cytokine production via metformin-mediated increase in glycolytic activity. …

    Scientific Reports 10(1), 14928, 2020-09-10

    IR 

  • Physiological mechanism of performance impairment during exercise with hypothermic skeletal muscle and application for exercise training  [in Japanese]

    若林 斉 , 坂上 輝将 , 仙石 泰雄 , 高木 英樹

    日本運動生理学雑誌 27(1), 9-13, 2020-03

  • Malic Enzyme Facilitates d-Lactate Production through Increased Pyruvate Supply during Anoxic Dark Fermentation in Synechocystis sp. PCC 6803

    Hidese Ryota , Matsuda Mami , Osanai Takashi , Hasunuma Tomohisa , Kondo Akihiko

    … Dynamic metabolomics revealed that overexpression of ME led to increased turnover rates in malate and pyruvate metabolism; … in contrast, deletion of ME resulted in increased pool sizes of glycolytic intermediates, probably due to sequential feedback inhibition, initially triggered by malate accumulation. …

    ACS Synthetic Biology 9(2), 2020-01-31

    IR 

  • Sirtuin 6 and metabolic genes interplay in Warburg effect in cancers

    Al-Azzam Nosayba

    … <p>Under oxygen availability, normal cells undergo mitochondrial oxidative phosphorylation to metabolize glucose and yield up to 36 ATPs per glucose molecule for cellular functions, and undergo non-oxidative metabolism (glycolysis) under hypoxic and proliferating conditions to yield 2 ATP per glucose. … Numerous glycolytic genes/proteins have been identified in tumors; … SIRT6 plays an important role in Warburg effect by regulating many glycolytic genes. …

    Journal of Clinical Biochemistry and Nutrition 66(3), 169-175, 2020

    J-STAGE  Ichushi Web 

  • Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells

    Haythorne Elizabeth , Rohm Maria , van de Bunt Martijn , Brereton Melissa F. , Tarasov Andrei I. , Blacker Thomas S. , Sachse Gregor , Silva dos Santos Mariana , Terron Exposito Raul , Davis Simon , Baba Otto , Fischer Roman , Duchen Michael R. , Rorsman Patrik , MacRae James I. , Ashcroft Frances M.

    … In isolated islets, glucose-induced increases in NADH and ATP are impaired and both oxidative and glycolytic glucose metabolism are reduced. … INS-1 β-cells cultured chronically at high glucose show similar changes in protein expression and reduced glucose-stimulated oxygen consumption: targeted metabolomics reveals impaired metabolism. … These data indicate hyperglycaemia induces metabolic changes in β-cells that markedly reduce mitochondrial metabolism and ATP synthesis. …

    Nature Communications (10), 2474, 2019-06-06

    IR 

  • Metabolic Oscillations and Their Biological Function  [in Japanese]

    雨宮 隆

    電子情報通信学会技術研究報告 = IEICE technical report : 信学技報 118(448), 9-11, 2019-02-15

  • The role of serine, a non-essential amino acid, in the production of cytokines  [in Japanese]

    Ohta Hiroya , Suganami Takayoshi , Kurita Kento , Shirakawa Ibuki , Okada Jun , Ohno Yuka , Ogino Atsushi , Tanaka Miyako , Arima Hiroshi , Ogawa Yoshihito

    … Metabolome analysis showed that depletion of serine affected cellular metabolism of MΦs. … We also found that cellular content of pyruvate, a crucial metabolite in the glycolytic pathway, was decreased. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 2-P-089, 2019

    J-STAGE 

  • Differential responses on energy metabolic pathway reprogramming between genotoxic and non-genotoxic hepatocarcinogens in rat liver cells

    Ito Yuko , Nakajima Kota , Masubuchi Yasunori , Kikuchi Satomi , Saito Fumiyo , Akahori Yumi , Jin Meilan , Yoshida Toshinori , Shibutani Makoto

    … To clarify difference in the responses on the reprogramming of metabolism toward carcinogenesis between genotoxic and non-genotoxic hepatocarcinogens in the liver, rats were repeatedly administered genotoxic hepatocarcinogens (<i>N-</i>nitrosodiethylamine, aflatoxin B<sub>1</sub>, <i>N</i>-nitrosopyrrolidine, or carbadox) or non-genotoxic hepatocarcinogens (carbon tetrachloride, thioacetamide, or methapyrilene hydrochloride) for 28, 84, or 90 days. …

    Journal of Toxicologic Pathology 32(4), 261-274, 2019

    J-STAGE  Ichushi Web 

  • Phenylbutyrate Ameliorates High-Fat Diet-Induced Obesity <i>via</i> Brown Adipose Tissue Activation

    Min Byong-Keol , Kang Hyeon-Ji , Choi Byung-Jun , Jeon Yong Hyun , Cho Je-Yoel , Lee In-Kyu , Kim Dong Wook

    … Interestingly, PBA could induce the expression of liver type phosphofructokinase (PFKL), a key enzyme in the glycolytic pathway, and knocking down PFKL dramatically repressed the expression level of <i>Ucp1</i> …

    Biological and Pharmaceutical Bulletin 42(9), 1554-1561, 2019

    J-STAGE  Ichushi Web 

  • Effects of muscle cooling on kinetics of pulmonary oxygen uptake and muscle deoxygenation at the onset of exercise

    高木 英樹 , Hitoshi Wakabayashi , Mizuki Osawa , Shunsaku Koga , Ke Li , Hiroyuki Sakaue , Yasuo Sengoku , Hideki TAKAGI

    … These results suggest that lower oxygen delivery to the hypothermic skeletal muscle might induce greater glycolytic metabolism during exercise and slower urn:x-wiley:2051817X:media:phy213910:phy213910-math-0003O2 kinetics at the onset of exercise. …

    Physiological reports 6(21), e13910, 2018-10

    IR 

  • A pyruvate carbon flux tugging strategy for increasing 2,3-butanediol production and reducing ethanol subgeneration in the yeast Saccharomyces cerevisiae

    Ishii Jun , Morita Keisuke , Ida Kengo , Kato Hiroko , Kinoshita Shohei , Hataya Shoko , Shimizu Hiroshi , Kondo Akihiko , Matsuda Fumio

    … Tugging flux at a central glycolytic overflow metabolism point arising from high glycolytic activity may substantially increase higher alcohol production in S. …

    Biotechnology for Biofuels (11), 180, 2018-06-26

    IR 

  • Synchronization of Glycolytic Oscillations in Cancer Cells  [in Japanese]

    雨宮 隆 , 柴田 賢一 , 高橋 純平

    電子情報通信学会技術研究報告 = IEICE technical report : 信学技報 118(2), 7-9, 2018-04-06

  • Synchronization of Glycolytic Oscillations in Cancer Cells  [in Japanese]

    雨宮 隆 , 柴田 賢一 , 高橋 純平

    電子情報通信学会技術研究報告 = IEICE technical report : 信学技報 118(1), 7-9, 2018-04-06

  • Ad4BP/SF-1 regulates cholesterol synthesis to boost the production of steroids

    Baba Takashi , Otake Hiroyuki , Inoue Miki , Sato Tetsuya , Ishihara Yasuhiro , Moon Ju-Yeon , Tsuchiya Megumi , Miyabayashi Kanako , Ogawa Hidesato , Shima Yuichi , Wang Lixiang , Sato Ryuichiro , Yamazaki Takeshi , Suyama Mikita , Nomura Masatoshi , Choi Man Ho , Ohkawa Yasuyuki , Morohashi Ken-ichirou

    … Recently, we showed that the steroidogenic cell-specific nuclear receptor Ad4BP/ SF-1, which regulates steroidogenic genes, also regulates housekeeping glycolytic genes. … To our knowledge, this study provides the first example where housekeeping and cell-specific metabolism are coordinated at the transcriptional level.This work was supported by Grants 16H05142 (K.M.), 17H06427 (K.M.), 16K08593 (T.B.), and 17J03270 (M.I.) from the Japan Society for the Promotion of Science (JSPS) KAKENHI; …

    Communications Biology (1), 18, 2018-03-22

    IR 

  • The combination of NAD+-dependent deacetylase gene deletion and the interruption of gluconeogenesis causes increased glucose metabolism in budding yeast

    Masumoto Hiroshi , Matsuyama Shigeru

    … Metabolic engineering focuses on rewriting the metabolism of cells to enhance native products or endow cells with the ability to produce new products. … Glycolysis manages the levels of various secondary metabolites by controlling the supply of glycolytic metabolites. …

    PLOS ONE 13(3), e0194942, 2018-03

    IR 

  • The combination of NAD+-dependent deacetylase gene deletion and the interruption of gluconeogenesis causes increased glucose metabolism in budding yeast

    Masumoto Hiroshi , Matsuyama Shigeru , 松山 茂

    … Metabolic engineering focuses on rewriting the metabolism of cells to enhance native products or endow cells with the ability to produce new products. … Glycolysis manages the levels of various secondary metabolites by controlling the supply of glycolytic metabolites. …

    PLOS ONE 13(3), e0194942, 2018-03

    IR 

  • Reprogrammed glucose metabolism promotes aggressiveness and metastasis in colon cancer

    Lin Cheng-Wei , Kuo Chih-Chia , Ling Hsiang-Hsi

    … Moreover, upregulation of glycolytic capacity and glycolysis genes was observed in metastatic colon cancer cells, compared with the parental cells. … Our data showed that aberrant glucose metabolism may provide attractive and effective therapeutic approaches to selectively target cancer cells.</p> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO4-6-4, 2018

    J-STAGE 

  • Mitochondrial dysfunction induced by ceramide accumulation is involved in the cytotoxicity of 19, 20-epoxydocosapentaenoic acid in H9c2 cells

    Endo Tomoko , Samokvalov Victor , Darwesh Ahmed M , Khey Kevin , El-Sherbeni Ahmed A , El-Kadi Ayman , Machida Takuji , Hirafuji Masahiko , Seubert John M

    … Epoxydocosapentanoic acids (EDPs) are lipid mediators produced from the metabolism of docosahexaenoic acids (DHA) by cytochrome P450 epoxygenase. … In this study, we investigated the mechanism of the effect of DHA and 19,20-EDP on H9c2 cells cultured under glycolytic and OXPHOS conditions.</p><p>Methods: H9c2 cells were cultured in DMEM containing either normal (25 mM) or low (5.5 mM) glucose to change the metabolic state. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018(0), PO2-3-21, 2018

    J-STAGE 

Page Top