Search Results 1-20 of 151

  • Current status and future prospects for cardiac regenerative medicine with human iPSCs  [in Japanese]

    関根 乙矢 , 遠山 周吾 , 福田 恵一

    循環器内科 = Cardioangiology 87(4), 432-438, 2020-04

  • Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells

    Kitajima Ryunosuke , Nakai Risako , Imamura Takuya , Kameda Tomonori , Kozuka Daiki , Hirai Hirohisa , Ito Haruka , Imai Hiroo , Imamura Masanori

    … Evolutionary developmental biology of our closest living relative, the chimpanzee (Pan troglodytes), is essential for understanding the origin of human traits. … Induced pluripotent stem cells (iPSCs) offer an alternative in vitro model system to investigate developmental events by overcoming the limitations of in vivo study. … Here, we generated chimpanzee iPSCs from adult skin fibroblasts and reconstructed early neural development using in vitro differentiation culture conditions. …

    Stem Cell Research (44), 2020-04


  • Human iPS cell-derived mural cells as an in vitro model of hereditary cerebral small vessel disease

    Yamamoto Yumi , Kojima Katsutoshi , Taura Daisuke , Sone Masakatsu , Washida Kazuo , Egawa Naohiro , Kondo Takayuki , Minakawa Eiko N. , Tsukita Kayoko , Enami Takako , Tomimoto Hidekazu , Mizuno Toshiki , Kalaria Raj N. , Inagaki Nobuya , Takahashi Ryosuke , Harada-Shiba Mariko , Ihara Masafumi , Inoue Haruhisa

    … Here, we aimed to establish an in vitro model of CADASIL, which can recapitulate those CADASIL phenotypes, using induced pluripotent stem cells (iPSCs). … iPSCs from three CADASIL patients with p.Arg182Cys, p.Arg141Cys and p.Cys106Arg mutations were differentiated into MCs and their functional and molecular profiles were compared. …

    Molecular Brain (13), 2020-03-19


  • Genome Engineering Evolves Brain Tumor Modeling

    KOGA Tomoyuki , CHEN Clark C. , FURNARI Frank B.

    … These tools have been applied to cancer modeling to understand the pathogenic effects of the growing catalog of mutations found in human cancers. … Pertaining to brain tumors, neural progenitor cells derived from human induced pluripotent stem cells (iPSCs) engineered with different combinations of genetic driver mutations observed in distinct molecular subtypes of glioblastomas, the most common form of primary brain cancer in adults, give rise to brain tumors when engrafted orthotopically in mice. …

    Neurologia medico-chirurgica, 2020


  • Modeling of psychiatric disorders using iPSC technology  [in Japanese]

    Nakazawa Takanobu , Hashimoto Ryota , Takuma Kazuhiro , Hashimoto Hitoshi

    … <p>iPSC technology has enabled us to more accurately model the pathology of human diseases in drug discovery research. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-S29-5, 2020


  • Assessment of developmental neurotoxicity using human iPS cells  [in Japanese]

    Kazunobu Tsunemoto , Yamada Shigeru , Kanda Yasunari

    … </p><p>Because current developmental neurotoxicity (DNT) guideline (OECD TG426) requires a lot of animals and costs, it is necessary to establish more predictable approach using human iPS cells (hiPSCs). … Here, we tried to search suitable structural and functional endpoints by evaluating antifouling agent, such as tributyltin (TBT), as a positive compound using hiPSCs, which was expected to provide novel human cell-based applications for alternative in vitro testing approaches. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-P-374, 2020


  • Development of Alveolar Epithelial Type II Cells from Human Induced Pluripotent Stem Cells  [in Japanese]

    Kayoko Tsuji , Kanda Yasunari

    … However, it is difficult to obtain human lung cells and culture the cells for a long-term period to explore the mechanistic approach for the adverse effects. … In this study, we tried to induce alveolar epithelial type II (AT2) cells from human induced pluripotent stem cells (iPSCs) using two-dimensional culture method. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-P-369, 2020


  • Development of human intestinal organoids from iPS cell technology  [in Japanese]

    Yamada Shigeru , Kanda Yasunari

    … </p><p>Here, we report the generation of intestinal organoids using human iPS cells (iPSCs) by sequential treatment with various cytokines and compounds. … </p><p>These data suggest that human iPSCs are successfully differentiated into intestinal organoids consisting of epithelial monolayers.</p> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-P-370, 2020


  • Regenerative Medicine and Drug Development using Stem Cells  [in Japanese]

    Okano Hideyuki

    … <p>There is an increasing interest in the induced pluripotent stem cells (iPSCs)-based regenerative medicine for various diseases. … In a series of our previous efforts, we have addressed the issues of safety and tumorigenesis using iPSCs-derived NS/PCs (iPSCs-NS/PCs). …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-JPS-4, 2020


  • Intestinal Organoids Generated from Human Pluripotent Stem Cells

    Tsuruta Satoru , Uchida Hajime , Akutsu Hidenori

    … <p>The gastrointestinal system is one of the most complex organ systems in the human body, and consists of numerous cell types originating from three germ layers. … Human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have remarkable developmental plasticity and possess the potential for a wide variety of applications. …

    JMA Journal 3(1), 9-19, 2020


  • Application of Induced Pluripotent Stem Cells in Drug Discovery and Regenerative Medicine  [in Japanese]

    MARUYAMA Yoshiaki , SHINAGAWA Kaori , TSUNODA Satoshi , SAIGO Yuka , NOZAKA Yuka , OKUDAIRA Shinichi , KAMEDA Takashi , HAYASHI Yoshiki , IZUMI Kazuki , MAKI Kazushige

    … <p>Since the first successful generation of induced pluripotent stem cells (iPSCs) by Yamanaka et al. … Expected applications in drug development include use of human-derived iPSCs for drug safety evaluation and patient-derived iPSCs for pathological analysis, and for exploration and development of therapeutic drugs. …

    Regulatory Science of Medical Products 10(1), 33-40, 2020


  • CRISPR-Cas3 induces broad and unidirectional genome editing in human cells

    Morisaka Hiroyuki , Yoshimi Kazuto , Okuzaki Yuya , Gee Peter , Kunihiro Yayoi , Sonpho Ekasit , Xu Huaigeng , Sasakawa Noriko , Naito Yuki , Nakada Shinichiro , Yamamoto Takashi , Sano Shigetoshi , Hotta Akitsu , Takeda Junji , Mashimo Tomoji

    … Here we demonstrate that type I-E CRISPR mediates distinct DNA cleavage activity in human cells. … As an example of potential therapeutic applications, we show Cas3-mediated exon-skipping of the Duchenne muscular dystrophy (DMD) gene in patient-induced pluripotent stem cells (iPSCs). …

    Nature communications (10), 2019-12-06


  • Transcriptional alteration and radiation effect in human induced pluripotent stem cells (iPSCs) and ectoderm tissues derived from iPSCs  [in Japanese]

    島田 幹男 , 塚田 海馬 , 三宅 智子

    放射線生物研究 = Radiation biology research communications : 放射線生物研究会機関誌 54(4), 266-280, 2019-12

  • Base-Resolution Methylome of Retinal Pigment Epithelial Cells Used in the First Trial of Human Induced Pluripotent Stem Cell-Based Autologous Transplantation

    Araki Hiromitsu , Miura Fumihito , Watanabe Akira , Morinaga Chikako , Kitaoka Fumiyo , Kitano Yuko , Sakai Noriko , Shibata Yumiko , Terada Motoki , Goto So , Yamanaka Shinya , Takahashi Masayo , Ito Takashi

    … The first-in-human trial of induced pluripotent stem cell (iPSC)-based autologous transplantation was successfully performed on a female patient with age-related macular degeneration. …

    Stem Cell Reports 13(4), 761-774, 2019-10-08


  • Elimination of protein aggregates prevents premature senescence in human trisomy 21 fibroblasts

    西村 健 , Nobutoshi Nawa , Katsuya Hirata , Keiji Kawatani , Toshihiko Nambara , Sayaka Omori , Kimihiko Banno , Chikara Kokubu , Junji Takeda , Ken NISHIMURA , Manami Ohtaka , Mahito Nakanishi , Daisuke Okuzaki , Hidetoshi Taniguchi , Hitomi Arahori , Kazuko Wada , Yasuji Kitabatake , Keiichi Ozono

    … However, whether and how this effect alters cellular homeostasis and long-term features of human disease are not fully understood. … Here, we aimed to investigate cellular stress responses in human trisomy syndromes, using fibroblasts and induced pluripotent stem cells (iPSCs). … Notably, 4-PBA treatment successfully prevented the progression of premature senescence in secondary fibroblasts derived from trisomy 21 iPSCs. …

    PLOS ONE 14(7), e0219592, 2019-07


  • Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation

    鄭 允文 , Li-Ping Liu , Yu-Mei Li , Ning-Ning Guo , Shu Li , Xiaolong Ma , Yi-Xuan Zhang , Yimeng Gao , Jian-Ling Huang , Dong-Xu Zheng , Lu-Yuan Wang , Hui Xu , Lijian Hui , Yunwen ZHENG

    … Induced pluripotent stem cells (iPSCs) are a promising melanocyte source as they propagate indefinitely and can be established from patients. … However, the in vivo functions of human iPSC-derived melanocytes (hiMels) remain unknown. …

    Cell Reports 27(2), 455-466.e5, 2019-04


  • Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

    Takaki Tadashi , Inagaki Azusa , Chonabayashi Kazuhisa , Inoue Keiji , Miki Kenji , Ohno Seiko , Makiyama Takeru , Horie Minoru , Yoshida Yoshinori

    … Induced pluripotent stem cells (iPSCs) from type 1 long QT (LQT1) patients can differentiate into cardiomyocytes (CMs) including ventricular cells to recapitulate the disease phenotype. … Cardiomyocyte monolayers constituted mostly of ventricular cells derived from LQT1-iPSCs showed prolonged AP duration (APD) (p value = 0.000096). …

    Stem Cells International (2019), 2019-03-06


  • InVitro Disease Modeling of Hermansky-Pudlak Syndrome Type 2Using Human Induced Pluripotent Stem Cell-Derived Alveolar Organoids

    Korogi Yohei , Gotoh Shimpei , Ikeo Satoshi , Yamamoto Yuki , Sone Naoyuki , Tamai Koji , Konishi Satoshi , Nagasaki Tadao , Matsumoto Hisako , Ito Isao , Chen-Yoshikawa Toyofumi F. , Date Hiroshi , Hagiwara Masatoshi , Asaka Isao , Hotta Akitsu , Mishima Michiaki , Hirai Toyohiro

    … Methods of generating and expanding AT2 cells derived from induced pluripotent stem cells (iPSCs) have been established and are expected to be applicable to disease modeling. … From an HPS type 2 (HPS2) patient, we established disease-specific iPSCs (HPS2-iPSCs) and their gene-corrected counterparts. …

    Stem Cell Reports 12(3), 431-440, 2019-03-05


  • Evaluation of developmental neurotoxicity using neural differentiation potency in human iPS cells  [in Japanese]

    Yamada Shigeru , Yamazaki Daiju , Kanda Yasunari

    … Due to complexity of human brain development and species differences, alternative in vitro testing using human cells, such as human iPS cells (iPSCs), has been expected in terms of cost and time-consuming. … We have focused on neural differentiation potency in iPSCs as an endpoint of DNT hazard evaluation. … 5-FU decreased ATP levels in iPSCs. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 3-P-127, 2019


  • Modeling Anderson-Fabry Disease with Human iPSCs Reveals Pathogenic Glycosphingolipid-Induced Cardiac Abnormalities  [in Japanese]

    Morihara Hirofumi , Tomoda Kiichiro , Wakabayashi Shigeo , Asahi Michio

    … Here, we employed human induced pluripotent stem cells (<b>iPSCs</b>) modified with clustered regularly interspaced short palindromic repeats interference (<b>CRISPRi</b>) to <u>model cardiac AFD </u><i><u>in vitro</u></i>. … Our study reinforces the usefulness of the CRISPRi iPSCs in modeling monogenic diseases and delineating mechanisms of the diseases.</p> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 92(0), 2-YIA-24, 2019


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