Search Results 1-20 of 2142

  • Estrogen-SIRT1 Axis Plays a Pivotal Role in Protecting Arteries Against Menopause-Induced Senescence and Atherosclerosis

    Sasaki Yuichi , Ikeda Yoshiyuki , Miyauchi Takahiro , Uchikado Yoshihiro , Akasaki Yuichi , Ohishi Mitsuru

    … of menopause-induced arterial senescence and atherosclerotic development.</p><p><b>Methods</b>: Twelve-week old female apolipoprotein E-knockout (ApoE-KO) mice underwent ovariectomy (OVX) or sham surgery.</p><p><b>Results</b>: SIRT1 expression and endothelial nitric oxide synthase (eNOS) activation in the aorta were significantly lower in OVX mice than they were in sham mice (OVX vs. sham, <i>n</i>=5 per …

    Journal of Atherosclerosis and Thrombosis 27(1), 47-59, 2020

    J-STAGE 

  • Exercise Preconditioning Protects against Acute Cardiac Injury Induced by Lipopolysaccharide Through General Control Nonderepressible 2 Kinase

    Sun Zhong-Guang , Lu Guo , Zhao Lin-Lin , Zhang Li-Zhen , Li Ai , Jing Jing , Xu Xin

    … = 40) and GCN2 knockout (KO) (<i>n</i> … = 40) mice were divided into four groups: control, LPS (L), exercise preconditioning (E), and exercise preconditioning LPS (EL). … Mice in the exercise groups performed exercise for eight weeks. … After exercise, all mice were given an equal volume of LPS or saline (10 μg/g). …

    International Heart Journal, 2020

    J-STAGE 

  • Enhanced lysosomal degradation maintains the quiescent state of neural stem cells

    Kobayashi Taeko , Piao Wenhui , Takamura Toshiya , Kori Hiroshi , Miyachi Hitoshi , Kitano Satsuki , Iwamoto Yumiko , Yamada Mayumi , Imayoshi Itaru , Shioda Seiji , Ballabio Andrea , Kageyama Ryoichiro

    … Furthermore, conditional knockout of TFEB, a lysosomal master regulator, delays NSCs quiescence in vitro and increases NSC proliferation in the dentate gyrus of mice. …

    Nature Communications (10), 2019-11-29

    IR 

  • Fat-specific protein 27α inhibits autophagy-dependent lipid droplet breakdown in white adipocytes

    Nakajima Shinsuke , Nishimoto Yuki , Tateya Sanshiro , Iwahashi Yasuyuki , Okamatsu-Ogura Yuko , Saito Masayuki , Ogawa Wataru , Tamori Yoshikazu

    … In FSP27 KO mice in which FSP27α and β are both depleted, WAT is characterized by multilocular LD formation, and by increased mitochondrial abundance and energy expenditure, whereas BAT conversely manifests large oligolocular LDs and reduced energy expenditure. … Materials and Methods We investigated the effects of autophagy in WAT and BAT of wild type (WT) and FSP27 knockout (KO) mice. …

    Journal of Diabetes Investigation 10(6), 1419-1429, 2019-11

    IR 

  • PKN1 kinase-negative knock-in mice develop splenomegaly and leukopenia at advanced age without obvious autoimmune-like phenotypes

    Siddique Salman Mahmud , Kubouchi Koji , Shinmichi Yuka , Sawada Nana , Sugiura Reiko , Itoh Yasushi , Uehara Shunsuke , Nishimura Kanae , Okamura Shunsuke , Ohsaki Hiroyuki , Kamoshida Shingo , Yamashita Yusuke , Tamura Shinobu , Sonoki Takashi , Matsuoka Hiroshi , Itoh Tomoo , Mukai Hideyuki

    … Protein kinase N1 (PKN1) knockout (KO) mice spontaneously form germinal centers (GCs) and develop an autoimmune-like disease with age. … Here, we investigated the function of PKN1 kinase activity in vivo using aged mice deficient in kinase activity resulting from the introduction of a point mutation (T778A) in the activation loop of the enzyme. … PKN1[T778A] mice reached adulthood without external abnormalities; …

    Scientific Reports (9), 13977, 2019-09-27

    IR 

  • Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia

    Suzuki Kodai , Okada Hideshi , Takemura Genzou , Takada Chihiro , Kuroda Ayumi , Yano Hirohisa , Zaikokuji Ryogen , Morishita Kentaro , Tomita Hiroyuki , Oda Kazumasa , Matsuo Saori , Uchida Akihiro , Fukuta Tetsuya , Sampei So , Miyazaki Nagisa , Kawaguchi Tomonori , Watanabe Takatomo , Yoshida Takahiro , Ushikoshi Hiroaki , Yoshida Shozo , Maekawa Yoichi , Ogura Shinji , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Internal Medicine Asahi University School of Dentistry , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of MedicineLaboratory of Molecular Biology Department of Biofunctional Analysis Gifu Pharmaceutical University , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Tumor Pathology Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Internal Medicine Asahi University School of Dentistry , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Clinical Laboratory Gifu University Hospital , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine , Department of Parasitology and Infectious Diseases Gifu University Graduate School of MedicineDomain of Integrated Life Systems Center for Highly Advanced Integration of Nano and Life Sciences (G-CHAIN) Gifu University , Department of Emergency and Disaster Medicine Gifu University Graduate School of Medicine

    … 20 mg/kg) was injected intraperitoneally into 9- to 12-week-old granulocyte colony-stimulating factor knockout (G-CSFKO) mice, which harbor few neutrophils, and littermate control mice; … in a second assay, mice were injected with the NE-inhibitor sivelestat (0.2 mg/kg) at 3, 6, 9, and 12 hours after LPS administration. …

    The American journal of pathology 189(8), 1526-1535, 2019-08

    IR 

  • Basal Autophagy Deficiency Causes Thyroid Follicular Epithelial Cell Death in Mice

    Kurashige Tomomi , Nakajima Yasuyo , Shimamura Mika , Matsuyama Mutsumi , Yamada Masanobu , Nakashima Masahiro , Nagayama Yuji

    … Therefore, we evaluated the effect of Atg5 gene knockout (KO) on thyroid morphology and function. … To this end, Atg5flox/flox mice were crossed with TPO-Cre mice, yielding the thyroid follicular epithelial cell (thyrocyte)‒specific ATG5-deficient mice (Atg5thyr-KO/KO). … Atg5thyr-KO/KO mice were born normally, and thyroid morphology, thyroid weights, and serum T4 and TSH levels were almost normal at 4 months. …

    Endocrinology 160(9), 2085-2092, 2019-07-17

    IR 

  • Renin-angiotensin system research : from molecules to the whole body

    Takimoto-Ohnishi Eriko , Murakami Kazuo

    The journal of physiological sciences 69(4), 581-587, 2019-07

  • Hepatic Expression of Selenium Binding Protein (SelenBP) in Female Mice and Its Inducibility by 2,3,7,8-Tetrachlorodibenzo-p-dioxin : Study with SelenBP1-Knockout Mice  [in Japanese]

    宋 穎霞 , 藤本 景子 , 黒瀬 厚 , 石田 卓巳 , 古賀 貴之 , 李 任時 , 武田 知起 , 武知 進士 , 山田 英之 , 田中 嘉孝 , 石井 祐次

    … Hepatic expression of selenium binding protein (SelenBP) in female mice and its inducibility by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was studied using wild-type (WT) C57BL/6J and SelenBP1-knockout mice. … Female WT mice (4 weeks old) received TCDD at the dose of 100 mg/kg (i.p.). … In addition, SelenBP1-KO mice received TCDD at the same dose as the WT. …

    福岡医学雑誌 = Fukuoka acta medica 110(2), 128-132, 2019-06-25

    IR 

  • Pathophysiological Role of TRPM2 in Age-Related Cognitive Impairment in Mice

    Kakae Masashi , Miyanohara Jun , Morishima Misa , Nagayasu Kazuki , Mori Yasuo , Shirakawa Hisashi , Kaneko Shuji

    … Therefore, we examined the effects of TRPM2 deletion in young (2–3 months) and older (12–24 months) mice. … Compared with young wild-type (WT) mice, middle-aged (12–16 months) WT mice showed working and cognitive memory dysfunction and aged (20–24 months) WT mice exhibited impaired spatial memory. … However, these characteristics were not seen in TRPM2 knockout (TRPM2-KO) mice. …

    Neuroscience (408), 204-213, 2019-06-01

    IR 

  • Environmental Electrophile-Mediated Toxicity in Mice Lacking Nrf2, CSE, or Both

    秋山 雅博 , 新開 泰弘 , 熊谷 嘉人 , Masahiro AKIYAMA , Takamitsu Unoki , Yasuhiro SHINKAI , Isao Ishii , Tomoaki Ida , Takaaki Akaike , Masayuki Yamamoto , Yoshito KUMAGAI

    … We also wished to clarify the molecular basis of the developmental window of toxicity through investigating expression levels of Nrf2, RSS-producing enzymes, and sulfur nucleophiles during developmental stages of mice.Methods:Wild-type (WT), CSE knockout (KO), Nrf2 KO, Nrf2/CSE double KO (DKO) mice, and their primary hepatocytes were analyzed in this study. …

    Environmental health perspectives 127(6), 067002, 2019-06

    IR 

  • Calaxin is required for cilia-driven determination of vertebrate laterality

    柴 小菊 , 馬場 忠 , Keita Sasaki , Kogiku SHIBA , Akihiro Nakamura , Natsuko Kawano , Yuhkoh Satouh , Hiroshi Yamaguchi , Motohiro Morikawa , Daisuke Shibata , Ryuji Yanase , Kei Jokura , Mami Nomura , Mami Miyado , Shuji Takada , Hironori Ueno , Shigenori Nonaka , Tadashi BABA , Masahito Ikawa , Masahide Kikkawa , Kenji Miyado , Kazuo Inaba

    … Here we show that the mice with a null mutation in Efcab1, which encodes calaxin, display typical phenotypes of primary ciliary dyskinesia, including hydrocephalus, situs inversus, and abnormal motility of trachea cilia and sperm flagella. … Strikingly, both males and females are viable and fertile, indicating that calaxin is not essential for fertilization in mice. …

    Communications Biology 2(226), 2019-06

    IR 

  • Remarkable complexity and variability of corticospinal tract defects in adult Semaphorin 6A knockout mice

    桝 正幸 , 岡田 拓也 , 桝 和子 , Takuya OKADA , Kazuko KEINO-MASU , Fumikazu Suto , Kevin J. Mitchell , Masayuki MASU

    … Previous studies have shown that postnatal Sema6A mutant mice have CST defects at the midbrain–hindbrain boundary and medulla. … In this study, we performed 3D reconstruction of immunostained CST fibers to reevaluate the details of the abnormal CST trajectories in the brains of adult Sema6A mutant mice. …

    Brain Research (1710), 209-219, 2019-05

    IR 

  • BMP4 regulates both podocyte injury and mesangial expansion in the diabetic nephropathy  [in Japanese]

    藤田 結衣 , 冨永 辰也 , 寒川 裕未 , 長井 幸二郎 , 安部 秀斉 , 土井 俊夫

    … To analyze the effect of BMP4 was investigated in vitro and in vivo using streptozotocin (STZ)-induced and Bmp4 heterozygous knockout (Bmp4+/-) diabetic mice or podocyte-specific Bmp4 knockout mice, and Bmp4-induced or podocyte-specific transgenic mice. …

    四国医学雑誌 = Shikoku acta medica 75(1・2), 55-62, 2019-04-25

    IR  Ichushi Web 

  • Basic and clinical research for Endocrine and Metabolic disorders by a clinician  [in Japanese]

    遠藤 逸朗

    … Specifically, I have identified that skeletal muscle differentiation and developmental abnormalities in vitamin D receptor knockout mice, activated calcium-sensing receptor knock-in mice showed various metabolic abnormalities, regulation of stem cell differentiation by IL-11 and so on. …

    四国医学雑誌 = Shikoku acta medica 75(1・2), 31-38, 2019-04-25

    IR  Ichushi Web 

  • Nonsense mutation in CFAP43 causes normal-pressure hydrocephalus with ciliary abnormalities

    Morimoto Yoshiro , Yoshida Shintaro , Kinoshita Akira , Satoh Chisei , Mishima Hiroyuki , Yamaguchi Naohiro , Matsuda Katsuya , Sakaguchi Miako , Tanaka Takeshi , Komohara Yoshihiro , Imamura Akira , Ozawa Hiroki , Nakashima Masahiro , Kurotaki Naohiro , Kishino Tatsuya , Yoshiura Koh-ichiro , Ono Shinji

    … We performed whole-exome sequencing (WES) on a Japanese family with multiple individuals with NPH and identified a candidate gene.Then we generated knockout mouse using CRISPR/Cas9 to confirm the effect of the candidate gene on the pathogenesis of hydrocephalus.RESULTS: In WES, we identified a loss-of-function variant in CFAP43 that segregated with the disease. …

    Neurology 92(20), e2364-e2374, 2019-04-19

    IR 

  • Data for 3D reconstruction of the corticospinal tract in the wild-type and Semaphorin 6A knockout adult brain

    岡田 拓也 , 桝 和子 , 桝 正幸 , Takuya OKADA , Kazuko KEINO-MASU , Fumikazu Suto , Kevin J. Mitchell , Masayuki MASU

    … Previous studies have shown that Sema6A knockout (KO) mice have CST defects at the midbrain–hindbrain boundary and medulla [1]. … Therefore here, to track the trajectory of the abnormal fibers, 3D images of the CST in adult mice were reconstructed from serial brain sections stained with anti-PKCγ antibody. …

    Data in Brief (23), 103718, 2019-04

    IR 

  • Microglial SIRPα regulates the emergence of CD11c(+) microglia and demyelination damage in white matter

    Sato-Hashimoto Miho , Nozu Tomomi , Toriba Riho , Horikoshi Ayano , Akaike Miho , Kawamoto Kyoko , Hirose Ayaka , Hayashi Yuriko , Nagai Hiromi , Shimizu Wakana , Saiki Ayaka , Ishikawa Tatsuya , Elhanbly Ruwaida , Kotani Takenori , Murata Yoji , Saito Yasuyuki , Naruse Masae , Shibasaki Koji , Oldenborg Per-Arne , Jung Steffen , Matozaki Takashi , Fukazawa Yugo , Ohnishi Hiroshi

    Mice lacking CD47, a physiological ligand of SIRPα, and microglia-specific SIRPα-knockout mice exhibited the same phenotype, suggesting that an interaction between microglial SIRPα and CD47 on neighbouring cells suppressed the emergence of CD11c(+) microglia. …

    eLife (8), e42025, 2019-03-26

    IR 

  • Mechanism of the conversion of vitamin K into menaquinone-4 and its physiological significance  [in Japanese]

    中川 公恵

    ビタミン = Vitamins 93(3), 93-102, 2019-03

    Ichushi Web 

  • Interleukin 1 upregulates microRNA-135b to promote inflammation-associated gastric carcinogenesis in mice.

    Tae Su Han , Voon Dominic Chih-Cheng , Oshima Hiroko , Nakayama Mizuho , Echizen Kanae , Sakai Eri , Yong Zachary Wei Ern , Murakami Kazuhiro , Yu Liang , Minamoto Toshinari , Ock Chan-Young , Jenkins Brendan J. , Kim Seong-Jin , Yang Han-Kwang , Oshima Masanobu

    Embargo Period 12 months

    Gastroenterology 156(4), 1140-1155, 2019-03

    IR  DOI 

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