Search Results 1-20 of 937

  • The Japanese Herbal (Kampo) Medicine Hochuekkito Attenuates Lung Inflammation in Lung Emphysema

    Isago Hideaki , Matsuzaki Hirotaka , Yatomi Yutaka , Nagase Takahide , Jo Taisuke , Mitani Akihisa , Kohno Shiho , Nagoshi Saki , Ishimori Taro , Saito Minako , Tamiya Hiroyuki , Miyashita Naoya , Ishii Takashi

    … using a mouse model of lung emphysema. … conditional knockout mice (<i>Taz</i> …

    Biological and Pharmaceutical Bulletin 44(1), 39-45, 2021

    J-STAGE 

  • Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes

    La The Mon , Tachibana Hiromi , Li Shun-Ai , Abe Tadashi , Seiriki Sayaka , Nagaoka Hikaru , Takashima Eizo , Takeda Tetsuya , Ogawa Daisuke , Makino Shin-Ichi , Asanuma Katsuhiko , Watanabe Masami , Tian Xuefei , Ishibe Shuta , Sakane Ayuko , Sasaki Takuya , Wada Jun , Takei Kohji , Yamada Hiroshi

    … By immunofluorescence of differentiated mouse podocytes (MPCs), dynamin 1 was often colocalized with microtubule bundles, which radially arranged toward periphery of expanded podocyte. … Dynamins 1 and 2 double-knockout podocytes showed dispersed acetylated alpha-tubulin and rare protrusions. …

    FASEB Journal 34(12), 16449-16463, 2020-10-17

    IR 

  • Identification of distinct loci for de novo DNA methylation by DNMT3A and DNMT3B during mammalian development

    Yagi Masaki , Kabata Mio , Tanaka Akito , Ukai Tomoyo , Ohta Sho , Nakabayashi Kazuhiko , Shimizu Masahito , Hata Kenichiro , Meissner Alexander , Yamamoto Takuya , Yamada Yasuhiro

    … Here, we analyze de novo DNA methylation in mouse embryonic fibroblasts (2i-MEFs) derived from DNA-hypomethylated 2i/L ES cells with genetic ablation of Dnmt3a or Dnmt3b. … We identify 355 and 333 uniquely unmethylated genes in Dnmt3a and Dnmt3b knockout (KO) 2i-MEFs, respectively. …

    Nature Communications (11), 2020-06-24

    IR 

  • Extracellular Vesicles Enriched with Moonlighting Metalloproteinase Are Highly Transmissive, Pro-Tumorigenic, and Trans-Activates Cellular Communication Network Factor (CCN2/CTGF) : CRISPR against Cancer

    Okusha Yuka , Eguchi Takanori , Tran Manh T. , Sogawa Chiharu , Yoshida Kaya , Itagaki Mami , Taha Eman A. , Ono Kisho , Aoyama Eriko , Okamura Hirohiko , Kozaki Ken-Ichi , Calderwood Stuart K. , Takigawa Masaharu , Okamoto Kuniaki

    … MMP3-rich, LuM1-derived EVs were disseminated to multiple organs through body fluid and were pro-tumorigenic in an allograft mouse model, which prompted us to define LuM1-EVs as oncosomes in the present study. … The CRISPR/Cas9-mediated knockout of MMP3 showed significant anti-tumor effects such as the inhibition of migration and invasion of tumor cells, and a reduction in CCN2/CTGF promoter activity and fragmentations in vitro. …

    Cancers 12(4), 881, 2020-04-04

    IR 

  • Assessment of Arf6 Deletion in PLB-985 Differentiated in Neutrophil-Like Cells and in Mouse Neutrophils: Impact on Adhesion and Migration

    船越 祐司 , 金保 安則 , Jouda Gamara , Lynn Davis , Emmanuelle Rollet-Labelle , Tsunaki Hongu , Yuji FUNAKOSHI , Yasunori KANAHO , Fawzi Aoudjit , Sylvain G. Bourgoin

    … In this study, we evaluated the role of Arf6 on PMN adhesion in vitro and on migration to inflammatory sites using PMN-Arf6 conditional knockout (cKO) mice. … Using the Elane-Cre deleting mouse strains, we report that the level of Arf6 deletion in inflammatory PMNs isolated from the dorsal air pouches was stronger when compared to naïve cells isolated from the bone marrow. …

    Mediators of inflammation (2020), 2713074, 2020-04

    IR 

  • Nanosecond pulsed electric fields induce the integrated stress response via reactive oxygen species-mediated heme-regulated inhibitor (HRI) activation

    Hamada Yoshimasa , Furumoto Yuji , Izutani Akira , Taniuchi Shusuke , Miyake Masato , Oyadomari Miho , Teranishi Kenji , Shimomura Naoyuki , Oyadomari Seiichi

    … To assess the responsible eIF2α kinase, we used previously established eIF2α kinase quadruple knockout (4KO) and single eIF2α kinase-rescued 4KO mouse embryonic fibroblast (MEF) cells. …

    PLOS ONE 15(3), e0229948, 2020-03-10

    IR 

  • The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components

    Teramoto Machiko , Sugawara Ryo , Minegishi Katsura , Uchikawa Masanori , Takemoto Tatsuya , Kuroiwa Atsushi , Ishii Yasuo , Kondoh Hisato

    … In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. … In the present study, we produced mouse embryos with AFG-specific SOX2 deficiency. …

    Biology Open 9(2), bio048728, 2020-02-07

    IR 

  • Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability

    Dina Mostafa , Akinori Takahashi , Akiko Yanagiya , Tomokazu Yamaguchi , Takaya Abe , Taku Kureha , Keiji Kuba , Yumi Kanegae , Yasuhide Furuta , Tadashi Yamamoto , Toru Suzuki

    … In this study, we addressed the contribution of CNOT7/8 to viability of primary mouse embryonic fibroblasts (MEFs). … We found that MEFs lacking CNOT7/8 expression [Cnot7/8-double knockout (dKO) MEFs] undergo cell death, whereas MEFs lacking CNOT6/6L expression (Cnot6/6l-dKO MEFs) remain viable. …

    RNA Biology 17(3), 403-416, 2020-01-10

    IR 

  • Abnormal Pyramidal Decussation and Bilateral Projection of the Corticospinal Tract Axons in Mice Lacking the Heparan Sulfate Endosulfatases, Sulf1 and Sulf2

    岡田 拓也 , 桝 和子 , 小金澤 禎史 , 秋山 雅博 , 玉岡 晃 , 桝 正幸 , Satoshi Aizawa , Takuya OKADA , Kazuko KEINO-MASU , Tri Huu Doan , Tadachika KOGANEZAWA , Masahiro AKIYAMA , Akira TAMAOKA , Masayuki MASU

    … Previously, we found that double-knockout (DKO) mouse embryos lacking the heparan sulfate endosulfatases, Sulf1 and Sulf2, showed axon guidance defects of the CST owing to the abnormal accumulation of Slit2 protein on the brain surface. …

    Frontiers in Molecular Neuroscience (12), 333, 2020-01

    IR 

  • Is age-related increase of chromosome segregation errors in mammalian oocytes caused by cohesin deterioration?

    Lee Jibak

    … Results Recent studies show that chromosome-associated cohesin decreases in an age-dependent manner in mouse oocytes. … Furthermore, conditional knockout or activation of cohesin in oocytes indicates that only the cohesin expressed before premeiotic S phase can establish and maintain sister chromatic cohesion and that cohesin does not turnover during the dictyate arrest. …

    Reproductive Medicine and Biology 19(1), 32-41, 2020-01

    IR 

  • Role of Metallothionein in Transcriptional Regulation by Metal-Responsive Element-Binding Transcription Factor 1

    Tanaka Yu-ki , Futami Yoshiaki , Fukumoto Yasunori , Suzuki Noriyuki , Ogra Yasumitsu

    … To investigate the potential role of MT in the inactivation of MTF-1, we examined the transcriptional levels of reporter and endogenous MRE-dependent genes using mouse embryonic fibroblasts (MEFs) established from MT-knockout (KO) and wild-type (WT) mice. …

    BPB Reports 3(1), 22-27, 2020

    J-STAGE 

  • Effect of Tricaprin on Cardiac Proteome in a Mouse Model for Triglyceride Deposit Cardiomyovasculopathy

    Hara Yasuhiro , Hirano Ken-ichi , Zhang Bo , Suzuki Akira , Yamaguchi Satoshi , Adachi Jun , Tomonaga Takeshi , Yasunaga Shin'ichiro , Saku Keijiro , Aoyama Toshiaki

    … We recently reported that tricaprin diet improved cardiac TG metabolism and left ventricular function in an ATGL-knockout (KO) mouse, a mouse model for TGCV. … These results indicated that tricaprin diet ameliorated HF in a TGCV mouse model at protein expression levels and also provided important clues to understand mechanisms for the beneficial effect of tricaprin.</p> …

    Journal of Oleo Science 69(12), 1569-1577, 2020

    J-STAGE 

  • Analysis of the sperm flagellar axoneme using gene-modified mice

    MIYATA Haruhiko , MOROHOSHI Akane , IKAWA Masahito

    … Although knockout (KO) mice have been generated to understand their function in the formation and motility regulation of sperm flagella, the majority of KO mice die before sexual maturation due to impaired ciliary motility, which makes it challenging to analyze mature spermatozoa. … In this review, we introduce methods that have been used to overcome premature lethality, focusing on KO mouse lines of central pair components.</p> …

    Experimental Animals 69(4), 374-381, 2020

    J-STAGE 

  • Deficiency of interleukin-19 exacerbates lipopolysaccharide/D-galactosamine-induced acute liver failure

    FUJIMOTO Yasuyuki , KUWAMURA Mitsuru , AZUMA Yasu-Taka

    … Here, we examined the effect of IL-19 on acute liver failure (ALF) using two mouse models of ALF: lipopolysaccharide and D-galactosamine (LPS/GalN)-induced model and concanavalin A (ConA)-induced model. …

    Journal of Veterinary Medical Science 82(10), 1450-1455, 2020

    J-STAGE 

  • Axdnd1 knockout mouse arise spermatogenesis failure  [in Japanese]

    HARIMA Ryua , HIRADATE Yuki , MATSUYAMA Makoto , FUJII Wataru , HARA Kenshiro , TANEMURA Kentaro

    <p>【目的】精子鞭毛は運動を担う重要な運動器官であり,主に軸糸ダイニンによって制御されている。軸糸ダイニンは分子量の大きさから,重鎖,中間鎖,軽鎖に分類される。このうち重鎖はATP分解活性を有し,鞭毛の滑り運動を司る一方で,中間鎖・軽鎖は重鎖機能を調節すると考えられているが,哺乳類精子では未解明の部分が多い。<i>Axdnd1</i>(axonemal dyn …

    The Journal of Reproduction and Development Supplement 113(0), P-24-P-24, 2020

    J-STAGE 

  • CRISPR-based genetic screen identified <i>Cox7b2</i> and <i>Gm773</i> as critical factors for the sperm fertilizing ability in mice  [in Japanese]

    AKTER Most Sumona , HADA Masashi , WATANABE Gen , OGURA Atsuo , MATOBA Shogo

    … [Materials and Methods] We selected five uncharacterized candidate genes (<i>Cox7b2</i>, <i>Cox8c</i>, <i>Faiml</i>, <i>Gm773</i>, and <i>Tuba3b</i>) and one reportedly positive control (<i>Majin</i>), all of which are exclusively expressed in the mouse testes by <i>in silico </i>screening. … We directly generated bi-allelic knockout (KO) mice for each candidate by the triple-target CRISPR-based method. …

    The Journal of Reproduction and Development Supplement 113(0), P-25-P-25, 2020

    J-STAGE 

  • Production of genome-edited mice by visualization of nucleases introduced into the embryos using electroporation

    WAKE Yui , KANEKO Takehito

    … Recently, a new electroporation technique (TAKE: Technique for animal knockout system by electroporation) was developed to produce genome-edited animals by introducing nucleases into intact embryos using electroporation instead of the microinjection method. …

    Journal of Reproduction and Development 66(5), 469-473, 2020

    J-STAGE 

  • Tamoxifen feeding method is suitable for efficient conditional knockout

    YOSHINOBU Kumiko , ARAKI Masatake , MORITA Ayaka , ARAKI Miyuki , KOKUBA Shun , NAKAGATA Naomi , ARAKI Kimi

    … <p>The Cre-driver system is used to generate conditional knockout mice. … Tamoxifen inducible Cre-driver mice can be used for spatiotemporal knockout by administration of the drug. … A mouse line expressing the tamoxifen-inducible <i>Cre</i> …

    Experimental Animals, 2020

    J-STAGE 

  • Role of T-type Calcium Channels in Regulating Neuronal Function  [in Japanese]

    Yabuki Yasushi

    … Recently, several reports suggest that T-type calcium channels may mediate neuronal plasticity in the mouse brain. … SAK3 can promote acetylcholine (ACh) release in the mouse hippocampus <i>via</i> … In this review, we have introduced the role of T-type calcium channel, especially Cav3.1 channel in the mouse hippocampus based on our previous data using SAK3 and Cav3.1 knockout mice.</p> …

    YAKUGAKU ZASSHI 140(10), 1207-1212, 2020

    J-STAGE 

  • SSEA-3 and 4 are not essential for the induction or properties of mouse iPS cells

    Hamamura Kazunori , Hotta Hiroshi , Murakumo Yoshiki , Shibuya Hidenobu , Kondo Yuji , Furukawa Koichi

    … This study investigated the biological and functional significance of globo-series glycolipids such as SSEA-3 and 4 in mouse iPS cells induced from tail-tip fibroblasts (TTFs) of α1,4Gal-T-knockout mice (lacking SSEA-3 and 4). … Furthermore, <i>in vitro</i>-differentiated induction experiments after embryoid body formation revealed that some colonies derived from α1, 4Gal-T knockout mice were able to differentiate into three germ layers. …

    Journal of Oral Science 62(4), 393-396, 2020

    J-STAGE 

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