Search Results 1-20 of 372

  • Anti–USAG-1 therapy for tooth regeneration through enhanced BMP signaling

    Murashima-Suginami A. , Kiso H. , Tokita Y. , Mihara E. , Nambu Y. , Uozumi R. , Tabata Y. , Bessho K. , Takagi J. , Sugai M. , Takahashi K.

    … Here, we show that blocking USAG-1 function through USAG-1 knockout or anti–USAG-1 antibody administration relieves congenital tooth agenesis caused by various genetic abnormalities in mice. … Our results demonstrate that USAG-1 controls the number of teeth by inhibiting development of potential tooth germs in wild-type or mutant mice missing teeth. …

    Science Advances 7(7), 2021-02-10

    IR 

  • Post-Germination Growth of Starch Branching Enzyme IIb Knockout Mutant of Rice Accumulating Resistant Starch  [in Japanese]

    Honda Sotaro , Sakoda Kazuma , Aoki Naohiro , Morita Ryutaro

    Abstracts of Meeting of the CSSJ 251(0), 122-122, 2021

    J-STAGE 

  • Two aquaporins, SIP1;1 and PIP1;2, mediate water transport for pollen hydration in the <i>Arabidopsis</i> pistil

    Windari Endang Ayu , Suwabe Keita , Ando Mei , Mizoguchi Yohei , Shimada Hiroto , Ohira Keima , Kagaya Yasuaki , Higashiyama Tetsuya , Takayama Seiji , Watanabe Masao

    … double knockout mutant showed a similar impairment of pollen hydration to individual single mutants, suggesting that their coordinated regulation is critical for proper water transport, in terms of speed and amount, in the pistil to accomplish successful pollen hydration.</p> …

    Plant Biotechnology 38(1), 77-87, 2021

    J-STAGE 

  • SIRT1, a histone deacetylase, mediates the proper DNA damage response via histone H2AX deacetylation in the cardiomyocyte.  [in Japanese]

    Kuno Atsushi , Hosoda Ryusuke , Horio Yoshiyuki

    … We recently found that knockout of SIRT1, a histone deacetylase, aggravates doxorubicin (DOX)-induced cardiotoxicity. … We hypothesized that SIRT1 regulates DNA damage response (DDR) to attenuate cardiotoxicity.</p><p><b>Methods and Results: </b>Wild type (WT) and SIRT1 knockout in the cardiomyocyte (SIRT1-cKO) mice were treated with vehicle or DOX (4 IP injections of 5 mg/kg/week) starting at 3-month-old. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-O-G5-5, 2021

    J-STAGE 

  • Phenotypic examination of the endocannabinoid-degrading enzyme alpha/beta-hydrolase domain containing 6 (ABHD6) knockout rat  [in Japanese]

    Noguchi Katsuhiko , Sugaya Kimio , Nishijima Saori , Sakanashi Mayuko , Kadekawa Katsumi , Okitsu Shiho , Nakamine Sayomi , Torihara Hidetsugu , Yamamoto Hideyuki

    … To examine the physiological and pathological roles of ABHD6 <i>in vivo</i>, we studied the phenotypic examination of Abhd6-knockout rats created by CRSPR/Cas9. Abhd6<sup>−/−</sup> and the age-matched wild type (WT) rats of both sexes were used. … Mutant rats generally looked normal in appearance, and the body weight and food intake were similar with WT rats. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-P1-09, 2021

    J-STAGE 

  • Disruption of glymphatic system and slow in waste clearance in the SOD1-G93A mouse model of ALS  [in Japanese]

    Mikako Hirose , Asano Mito , Matsumoto Saori , Yamanaka Koji , Abe Yoichiro , Yasui Masato , Tokuda Eiichi , Furukawa Yoshiaki , Misawa Hidemi

    … Accumulation of mutant Cu/Zn-superoxide dismutase (SOD1) protein aggregate in the spinal motor neurons is a common pathological hallmark in several types of ALS animal models and patients. … In addition, abnormal SOD1 protein deposition was increased in SOD1-ALS/AQP4 knockout mice and the clearance of the protein from the spinal cord was slowed in AQP4 knockout mice. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 2-O-B3-1, 2021

    J-STAGE 

  • Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii

    Xu Nannan , Oltmanns Anne , Zhao Longsheng , Girot Antoine , Karimi Marzieh , Hoepfner Lara , Kelterborn Simon , Scholz Martin , Beissel Julia , Hegemann Peter , Baeumchen Oliver , Liu Lu-Ning , Huang Kaiyao , Hippler Michael

    … reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions. …

    eLife (9), e58805, 2020-12-10

    IR 

  • Impairment of cerebellar long‑term depression and GABAergic transmission in prion protein deficient mice ectopically expressing PrPLP/Dpl

    Kishimoto Yasushi , Hirono Moritoshi , Atarashi Ryuichiro , Sakaguchi Suehiro , Yoshioka Tohru , Katamine Shigeru , Kirino Yutaka

    … Prion protein (PrPC) knockout mice, named as the "Ngsk" strain (Ngsk Prnp0/0 mice), show late-onset cerebellar Purkinje cell (PC) degeneration because of ectopic overexpression of PrPC-like protein (PrPLP/Dpl). … Our previous study indicated that the mutant mice also exhibited alterations in cerebellum-dependent delay eyeblink conditioning, even at a young age (16 weeks of age) when neurological changes had not occurred. …

    Scientific Reports (10), 15900, 2020-09-28

    IR 

  • N-Terminal Regions of Prion Protein : Functions and Roles in Prion Diseases

    Hara Hideyuki , Sakaguchi Suehiro

    … Reverse genetic studies using reconstituted PrPC-knockout mice with various mutant PrP molecules have revealed that the N-terminal domain has an important role in the normal function of PrPC and the conversion of PrPC to PrPSc. …

    International Journal of Molecular Sciences 21(17), 6233, 2020-08-28

    IR 

  • Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice

    Fujihara Yoshitaka , Lu Yonggang , Noda Taichi , Oji Asami , Larasati Tamara , Kojima-Kita Kanako , Yu Zhifeng , Matzuk Ryan M. , Matzuk Martin M. , Ikawa Masahito

    … In this study, we focused on analyzing a testis-specific gene 4930451I11Rik and generated mutant mice using the CRISPR/Cas9 system. … Although IZUMO1 remained in 4930451I11Rik knockout (KO) spermatozoa, the KO spermatozoa were unable to fuse with oocytes and the KO males were severely subfertile. …

    Proceedings of the National Academy of Sciences of the United States of America 117(17), 9393-9400, 2020-04-28

    IR 

  • Construction of hypervesiculation Escherichia coli strains and application for secretory protein production

    Ojima Yoshihiro , Sawabe Tomomi , Konami Katsuya , Azuma Masayuki

    … The nlpI and mlaE double-gene-knockout mutant (ΔmlaEΔnlpI) showed the highest OMV production. …

    Biotechnology and Bioengineering 117(3), 701-709, 2020-02-11

    IR 

  • Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability

    Dina Mostafa , Akinori Takahashi , Akiko Yanagiya , Tomokazu Yamaguchi , Takaya Abe , Taku Kureha , Keiji Kuba , Yumi Kanegae , Yasuhide Furuta , Tadashi Yamamoto , Toru Suzuki

    … We found that MEFs lacking CNOT7/8 expression [Cnot7/8-double knockout (dKO) MEFs] undergo cell death, whereas MEFs lacking CNOT6/6L expression (Cnot6/6l-dKO MEFs) remain viable. … Exogenous expression of a CNOT7 mutant lacking catalytic activity in Cnot7/8-dKO MEFs cannot recover cell viability, even though CNOT6/6L exists to some extent in the CCR4-NOT complex, confirming that CNOT7/8 is essential for viability. …

    RNA Biology 17(3), 403-416, 2020-01-10

    IR 

  • Involvement of NMDA receptors in tremor expression in <i>Aspa</i>/<i>Hcn1</i> double-knockout rats

    NISHITANI Ai , NAGAYOSHI Haruna , TAKENAKA Shigeo , ASANO Masahide , SHIMIZU Saki , OHNO Yukihiro , KURAMOTO Takashi

    … double-mutant rats, but they were bred on a heterogeneous genetic background of two strains, F344 and WTC. … double-knockout rat strain with a homogenous F344 genetic background and studied the ability of glutamate receptor antagonists to suppress ET. … double-knockout rats exhibited spontaneous, intense body tremor equivalent to that in the double-mutant rats. …

    Experimental Animals 69(4), 388-394, 2020

    J-STAGE 

  • The Role of Cell Polarity Regulator aPKC in Hair Follicle Stem Cell Maintenance and Wound Healing  [in Japanese]

    Osada Shin-Ichi

    … To clarify functional differences between aPKCζ and aPKCλ in the stratified epidermis <i>in vivo</i>, we have generated mutant mice with genetic deletion of each aPKC isoform. … Epidermis-specific aPKCλ conditionally knockout mice (aPKCλ cKO) showed progressive hair loss, abnormal hair cycling, a gradual decrease of hair follicle stem cell (HFSC) population, and loss of the HFSC quiescence. …

    Nihon Ika Daigaku Igakkai Zasshi 16(3), 130-137, 2020

    J-STAGE  Ichushi Web 

  • Elucidation of Pathological Mechanisms for Involvement of Gangliosides in Intractable Diseases  [in Japanese]

    Ohmi Yuhsuke , Furukawa Keiko , Furukawa Koichi

    <p>酸性スフィンゴ糖脂質、ガングリオシドは、神経組織やがん組織に多く発現し、組織の健常性を制御している。我々は、ガングリオシド合成酵素をノックアウトする事により、種々のガングリオシドを欠損する変異マウスを作製し、様々な神経変性が起こることを報告した。その機構の一つとして、ガングリオシド欠損による脂質ラフトの構築異常が、グリア細胞の活性化を伴う慢性的な炎症を惹起し、神経変性を誘導するこ …

    Trends in Glycoscience and Glycotechnology 32(187), J67-J72, 2020

    J-STAGE 

  • Elucidation of Pathological Mechanisms for Involvement of Gangliosides in Intractable Diseases

    Ohmi Yuhsuke , Furukawa Keiko , Furukawa Koichi

    … We generated mutant mice lacking various gangliosides by knocking out ganglioside synthase genes, and reported that these knockout (KO) mice exhibited various neurodegeneration. …

    Trends in Glycoscience and Glycotechnology 32(187), E81-E86, 2020

    J-STAGE 

  • Inactivation of retrotransposon <i>Tos17</i><sup>Chr.7</sup> in rice cultivar Nipponbare through CRISPR/Cas9-mediated gene editing

    Luo Yanchang , Tian Dongsheng , Teo Joanne Chin Yi , Ong Kar Hui , Yin Zhongchao

    knockout mutant D873 by using CRISPR/Cas9 gene editing system. …

    Plant Biotechnology 37(1), 69-75, 2020

    J-STAGE 

  • Involvement of glymphatic system in amyotrophic lateral sclerosis pathology  [in Japanese]

    Mikako Hirose , Asano Mito , Shitara Shusei , Abe Yoichiro , Yasui Masato , Tokuda Eiichi , Furukawa Yoshiaki , Misawa Hidemi

    … Accumulation of mutant Cu/Zn-superoxide dismutase (SOD1) protein aggregate in the spinal motor neurons is a common pathological hallmark in several types of ALS animal models and patients. … In the present study, we found the increase in the abnormal SOD1 protein deposition in SOD1-ALS/AQP4 knockout mice and the clearance of the protein from the spinal cord was slowed in AQP4 knockout mice. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 2-YIA-32, 2020

    J-STAGE 

  • SIRT1, an NAD<sup>+</sup>-dependent protein deacetylase, maintains oxidative muscle fiber in the skeletal muscle and contributes to exercise capacity in mice.  [in Japanese]

    Asakura Seidai , Hosoda Ryusuke , Kuno Atsushi , Horio Yoshiyuki

    … Immunohistological analysis using soleus muscle sections showed that the percentage of type IIa, one of oxidative muscle fibers, was significantly lower in the skeletal muscle-specific SIRT1 knockout mouse (SIRT1MKO) than that in WT (42% vs. 56%) at 79-98 weeks of age. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-SS-09, 2020

    J-STAGE 

  • Promoted performance of microbial fuel cells using Escherichia coli cells with multiple-knockout of central metabolism genes

    Ojima Yoshihiro , Kawaguchi Taichi , Fukui Saki , Kikuchi Ryota , Terao Kazuma , Koma Daisuke , Ohmoto Takashi , Azuma Masayuki

    … Next, multiple-gene knockout mutants were constructed using P1 transduction. … Our results show that the combination of multiple-gene knockout in E. …

    Bioprocess and Biosystems Engineering 43(2), 323-332, 2019-10-12

    IR 

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