Search Results 1-20 of 546

  • Elucidation of Molecular Mechanism of a Selective PPARα Modulator, Pemafibrate, through Combinational Approaches of X-ray Crystallography, Thermodynamic Analysis, and First-Principle Calculations

    島野 仁 , Mayu Kawasaki , Akira Kambe , Yuta Yamamoto , Sundaram Arulmozhiraja , Sohei Ito , Yoshimi Nakagawa , Hiroaki Tokiwa , Shogo Nakano , Hitoshi SHIMANO

    … Recently, pemafibrate was screened from the ligand library as an SPPARMα bearing strong potency. … The crystal structure of PPARα-ligand binding domain (LBD)/pemafibrate/steroid receptor coactivator-1 peptide (SRC1) determined at 3.2 Å resolution indicates that pemafibrate binds to the ligand binding pocket (LBP) of PPARα in a Y-shaped form. …

    International Journal of Molecular Sciences 21(1), 361, 2020-01


  • A new class of drug modalities based on molecular-targeting helix-loop-helix (HLH) peptides  [in Japanese]

    Fujii Ikuo , Fujiwara Daisuke , Michigami Masataka

    近年、低分子化抗体がポスト抗体医薬として注目されている。筆者らは、抗体様活性をもつ中分子の創薬モダリティーとして、ヘリックス・ループ・ヘリックス構造をもつ分子標的HLHペプチド(分子量:約4k)の開発を進めている。ファージ表層や酵母表層提示ライブラリーを構築し、進化分子工学的手法により、さまざまな疾患関連タンパク質に対する分子標的ペプチドを開発している。このペプチドは、強固な立体構造をもつため生体 …

    Drug Delivery System 35(3), 212-221, 2020

    J-STAGE  Ichushi Web 

  • Potent Antibacterial Activity of Synthetic Peptides Designed from Salusin-β and HIV-1 Tat(49–57)

    Kimura Masahiro , Kosuge Kumiko , Ko Yui , Kurosaki Nodoka , Tagawa Noriko , Kato Ikuo , Uchida Yoshiki

    … <p>Salusin-β is an endogenous bioactive peptide that was identified in a human full-length enriched cDNA library using bioinformatics analyses. … Salusin-β has an ability to depolarize the cytoplasmic membrane of this bacterium, and this phenomenon may be linked to the antibacterial activity of this peptide. …

    Chemical and Pharmaceutical Bulletin 68(8), 810-813, 2020


  • Identification of Receptors of Plant Peptide Hormones by Photoaffinity Labeling  [in Japanese]

    Shinohara Hidefumi , Matsubayashi Yoshikatsu

    … <p>In plants, there are several kinds of intercellular signal transduction systems, such as with phytohormones and with peptide hormones. … Small post-translationally modified peptides, which act as cell-to-cell signaling molecules, constitute the largest group of peptide hormones with various functions in intercellular signal transductions. … We explored peptide hormones by <i>in silico</i> … gene screening coupled with biochemical peptide detection. …

    Journal of Synthetic Organic Chemistry, Japan 78(7), 713-722, 2020


  • Detection of Abacavir-Induced Structural Alterations in Human Leukocyte Antigen-B*57 : 01 Using Phage Display

    Shirayanagi Tomohiro , Aoki Shigeki , Fujimori Sota , Watanabe Kenji , Aida Tetsuo , Hirasawa Makoto , Kumagai Kazuyoshi , Hoshino Tyuji , Ito Kousei

    … several different mechanisms such as the hapten theory, p-i concept, and altered peptide repertoire model, leading to the activation of cytotoxic T cells. … Here, we evaluated structural alterations in HLA complexes focusing on the interaction between the HLA-B*57 : 01 allele and abacavir (an anti-human immunodeficiency virus drug), representing a model of abacavir hypersensitivity syndrome induced by changes in the peptide repertoire on the HLA molecule. …

    Biological and Pharmaceutical Bulletin 43(6), 1007-1015, 2020


  • Development of Surface Modification Methods Based on Specific Affinities of Polymer–binding Peptides  [in Japanese]

    Sawada Toshiki , Serizawa Takeshi

    … The bioinspired peptide screening technology using phage display methods has been developed based on the evolution to generate functional peptides. … The polymer– binding peptides were obtained from a biologically constructed phage–displayed peptide library to recognize polymeric nanostructures, and were utilized for possible applications as novel biomolecular tools for functional polymers. …

    MEMBRANE 45(3), 100-107, 2020


  • Drug treatment options for the 2019-new coronavirus (2019-nCoV)

    Lu Hongzhou

    … Three general methods, which include existing broad-spectrum antiviral drugs using standard assays, screening of a chemical library containing many existing compounds or databases, and the redevelopment of new specific drugs based on the genome and biophysical understanding of individual coronaviruses, are used to discover the potential antiviral treatment of human pathogen coronavirus. …

    BioScience Trends 14(1), 69-71, 2020


  • Identification of the Epitope Recognized by the Anti-Red Sea Bream Iridovirus (RSIV) Monoclonal Antibody M10 Using a Phage Display RSIV Peptide Library

    Takano Tomokazu , Matsuyama Tomomasa , Kawato Yasuhiko , Sakai Takamitsu , Kurita Jun , Matsuura Yuta , Terashima Sachiko , Nakajima Kazuhiro , Nakayasu Chihaya

    … A phage display RSIV peptide library was constructed to cover the entire genome of RSIV, then phage clones recognized by the antibody were selected by biopanning. …

    Fish Pathology 54(4), 83-92, 2020


  • A combined strategy of neuropeptide prediction and tandem mass spectrometry identifies evolutionarily conserved ancient neuropeptides in the sea anemone Nematostella vectensis

    Eisuke Hayakawa , Hiroshi Watanabe , Gerben Menschaert , Thomas W. Holstein , Geert Baggerman , Liliane Schoofs

    … We developed a pipeline consisting of in silico prediction of candidate neuropeptides followed by peptide-spectrum matching. … This approach enables highly sensitive and reliable neuropeptide identification, as the search space for peptide-spectrum matching is highly reduced. … Peptide-spectrum matching was performed against a dataset of peptides that are cleaved in silico from these putative peptide precursors. …

    PLOS ONE 14(9), e0215185, 2019-09-23


  • High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics

    Yaginuma Kenshi , Aoki Wataru , Miura Natsuko , Ohtani Yuta , Aburaya Shunsuke , Kogawa Masato , Nishikawa Yohei , Hosokawa Masahito , Takeyama Haruko , Ueda Mitsuyoshi

    ナノバイオ手法による創薬ターゲットGPCRの機能性リガンド探索のハイスループット化に成功 --ドロップレットマイクロ流体デバイスの活用--. 京都大学プレスリリース. 2019-08-02.

    Scientific Reports (9), 2019-07-29


  • Amino acid signatures of HLA Class-I and II molecules are strongly associated with SLE susceptibility and autoantibody production in Eastern Asians

    Molineros Julio E. , Looger Loren L. , Kim Kwangwoo , Okada Yukinori , Terao Chikashi , Sun Celi , Zhou Xu-jie , Raj Prithvi , Kochi Yuta , Suzuki Akari , Akizuki Shuji , Nakabo Shuichiro , Bang So-Young , Lee Hye-Soon , Kang Young Mo , Suh Chang-Hee , Chung Won Tae , Park Yong-Beom , Choe Jung-Yoon , Shim Seung-Cheol , Lee Shin-Seok , Zuo Xiaoxia , Yamamoto Kazuhiko , Li Quan-Zhen , Shen Nan , Porter Lauren L. , Harley John B. , Chua Kek Heng , Zhang Hong , Wakeland Edward K. , Tsao Betty P. , Bae Sang-Cheol , Nath Swapan K.

    … Here, we dissected (a) overall SLE association signals across HLA, (b) HLA-peptide interaction, and (c) residue-autoantibody association. … DRB1-30, P = 3.9x10-14) localized to the peptide-binding groove of HLA-DRB1. … Enrichment for specific amino-acid characteristics in the peptide-binding groove correlated with overall SLE risk and with autoantibody presence. …

    PLOS Genetics 15(4), 2019-04-25


  • Peptide barcoding for establishment of new types of genotype–phenotype linkages

    Miyamoto Kana , Aoki Wataru , Ohtani Yuta , Miura Natsuko , Aburaya Shunsuke , Matsuzaki Yusei , Kajiwara Kaho , Kitagawa Yoshinori , Ueda Mitsuyoshi

    … In this paper, we propose a novel methodology, peptide barcoding, to quantitatively measure the binding properties of multiple binders without immobilization. … In the experimental scheme, unique peptide barcodes are fused with each binder, and they represent genotype information. … These peptide barcodes are designed to have high detectability for mass spectrometry, leading to low identification bias and a high identification rate. …

    PLOS ONE 14(4), 2019-04-23


  • Control of proliferation in the haploid meristem by CLE peptide signaling in Marchantia polymorpha

    Hirakawa Yuki , Uchida Naoyuki , Yamaguchi Yasuka L. , Tabata Ryo , Ishida Sakiko , Ishizaki Kimitsune , Nishihama Ryuichi , Kohchi Takayuki , Sawa Shinichiro , Bowman John L.

    … The homeostasis of meristems in flowering plants is maintained by cell-to-cell communication via CLE (CLAVATA3/EMBRYO SURROUNDING REGION-related) peptide hormones. … These data suggest that local CLE peptide signaling may have had a role in regulating cell proliferation in the shoot meristem in the ancestral land plant and acts in both sporophytic and gametophytic meristems of extant plants. …

    PLoS Genetics 15(3), e1007997, 2019-03-07


  • Factors Associated with Increased Levels of Brain Natriuretic Peptide and Cardiac Troponin I during the Peripartum Period

    Kimura Yuki , Kato Takao , Miyata Hiromi , Sasaki Issei , Minamino-Muta Eri , Nagasawa Yoshinori , Numao Shigeharu , Nagano Tadayoshi , Higuchi Toshihiro , Inoko Moriaki

    … Background: We aimed to investigate the values and the changes of brain natriuretic peptide (BNP) and cardiac troponin in pregnant women. …

    PLOS ONE 14(2), 2019-02-07


  • Design of Peptide Catalysts by Combining Secondary Structural Units for Selective Reactions  [in Japanese]

    Akagawa Kengo , Kudo Kazuaki

    … Most of the reported peptide catalysts consist of a few amino acid residues, and their structures are relatively simple. … Our group designed peptide catalysts by combining multiple secondary structural units, and found that these peptides with turn and helical motifs were effective to promote various amine-catalyzed reactions enantioselectively. … We also explored novel peptide scaffolds by the screening of peptide libraries. …

    Journal of Synthetic Organic Chemistry, Japan 77(12), 1222-1233, 2019


  • Combinatorial Synthesis and Biological Evaluation of Destruxins

    Yoshida Masahito

    … In addition, the preparation of a combinatorial library based on the structure of destruxins was carried out by the split-and-pool method. …

    Chemical and Pharmaceutical Bulletin 67(10), 1023-1029, 2019

    IR  J-STAGE  Ichushi Web 

  • Peptide Screening from a Phage Display Library for Benzaldehyde Recognition

    Tanaka Masayoshi , Minamide Taisuke , Takahashi Yuta , Hanai Yosuke , Yanagida Takeshi , Okochi Mina

    … <p>Peptides are promising probes for sensor development, while peptide screening for volatile organic compound (VOC) binding remains a challenge. … In this study, the peptides binding to benzaldehyde were screened from a phage display library. … The peptide sequences showing the highest binding activity were NPAATMA, SIFPVSR, and MPRLPPA. … The binding in the gas phase was also confirmed using a candidate peptide-immobilized ZnO nanowire structure. …

    Chemistry Letters 48(8), 978-981, 2019


  • Independent association of HLA-DPB1*02:01 with rheumatoid arthritis in Japanese populations

    古川 宏 , Hiroshi FURUKAWA , Shomi Oka , Kota Shimada , Atsushi Hashimoto , Akiko Komiya , Shinichiro Tsunoda , Akiko Suda , Satoshi Ito , Koichiro Saisho , Masao Katayama , Satoshi Shinohara , Takeo Sato , Katsuya Nagatani , Seiji Minota , Toshihiro Matsui , Naoshi Fukui , Shoji Sugii , Hajime Sano , Kiyoshi Migita , Shouhei Nagaoka , Shigeto Tohma

    … The production of anti-citrullinated peptide antibodies (ACPA) is specifically associated with RA. …

    PLOS ONE 13(9), e0204459, 2018-09


  • Midgut-derived neuropeptide F controls germline stem cell proliferation in a mating-dependent manner

    Ameku Tomotsune , Yoshinari Yuto , Texada Michael J. , Kondo Shu , Amezawa Kotaro , Yoshizaki Goro , Shimada-Niwa Yuko , Niwa Ryusuke , 島田 裕子 , 丹羽 隆介

    … NPF expressed in enteroendocrine cells (EECs) of the midgut is released in response to the seminal-fluid protein sex peptide (SP) upon mating. …

    PLoS biology 16(9), e2005004, 2018-09


  • Rats deficient C-type natriuretic peptide suffer from impaired skeletal growth without early death

    Fujii Toshihito , Hirota Keisho , Yasoda Akihiro , Takizawa Akiko , Morozumi Naomi , Nakamura Ryuichi , Yotsumoto Takafumi , Kondo Eri , Yamashita Yui , Sakane Yoriko , Kanai Yugo , Ueda Yohei , Yamauchi Ichiro , Yamanaka Shigeki , Nakao Kazumasa , Kuwahara Koichiro , Jindo Toshimasa , Furuya Mayumi , Mashimo Tomoji , Inagaki Nobuya , Serikawa Tadao , Nakao Kazuwa

    … We have previously investigated the physiological role of C-type natriuretic peptide (CNP) on endochondral bone growth, mainly with mutant mouse models deficient in CNP, and reported that CNP is indispensable for physiological endochondral bone growth in mice. …

    PLOS ONE 13(3), e0194812, 2018-03-22


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