Search Results 1-20 of 992

  • Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet

    Raju Resmi , Oshima Masamitsu , Inoue Miho , Morita Tsuyoshi , Huijiao Yan , Waskitho Arief , Baba Otto , Inoue Masahisa , Matsuka Yoshizo

    Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. … Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. …

    Scientific Reports (10), 1656, 2020-02-03


  • Function of MMP in periodontal ligament cells irradiated with Er:YAG laser  [in Japanese]

    NIWA Takahiko , KOBAYASHI Kazuyuki , YAMAKAWA Shunjiro , YAMAMOTO Ryuji , NAGANO Takatoshi , YAMAKOSHI Yasuo , GOMI Kazuhiro

    … Our previous study has revealed that the LLLT with Er:YAG laser enhanced the activity of transforming growth factor beta (TGF-β) in human periodontal ligament (HPDL) cells (Kobayashi et al., 2017). … However, it is unclear how the Er:YAG irradiation facilitates the TGF-β activity in HPDL cells. …

    Journal of Japanese Society for Laser Dentistry 30(1), 1-6, 2020


  • Comparison of the vertical bone defect healing abilities of carbonate apatite, β-tricalcium phosphate, hydroxyapatite and bovine-derived heterogeneous bone

    SATO Nobuya , HANDA Keisuke , VENKATAIAH Venkata Suresh , HASEGAWA Tatsuya , NJUGUNA Mary M. , YAHATA Yoshio , SAITO Masahiro

    … <p>The treatment of vertical bone defects caused by severe periodontal disease requires the regeneration of periodontal tissue. … Although various bone substitutes have been clinically applied to vertical bone defect correction, the evaluation of these materials in periodontal tissue remains incomplete. … All of these bone substitutes showed a similar healing ability to periodontal ligament and cementum. …

    Dental Materials Journal 39(2), 309-318, 2020


  • Effective root planing for biologically acceptable root surfaces: an experimental study

    Kano Chihiro , Kobayashi Hiroaki , Nozaki Kosuke , Tsumanuma Yuka , Sudo Takeaki , Khemwong Thatawee , Mikami Ryo , Izumi Yuichi

    <p>歯周治療におけるルートプレーニングの目的は, 露出歯根面の炎症起因物質を除去することである. しかしながら, 露出歯根面のPAMPsやDAMPsの浸透度はよく分かっていない. また, 歯周再生療法において, 歯根面から歯根膜幹細胞が受ける影響にも不明な点が多い. 本研究では, 細胞為害性物質の浸透度を計測し, また, 歯根膜幹細胞が露出歯根面から受ける影響を検討し, ルートプレー …

    Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) 62(1), 1-15, 2020

    J-STAGE  Ichushi Web 

  • QSAR analysis of tumor-specificity of newly synthesized 3-styrylchromone derivatives against human oral squamous cell carcinoma cell lines  [in Japanese]

    Sakagami Hiroshi , Sugita Yoshiaki , Shi Haixia , Uesawa Yoshihiro , Nagai Junko , Bandow Kenjiro , Tomomura Akito , Tomomura Mineko , Hoshi Kaori , Takao Koichi

    … Tumor-specificity (TS) was calculated by dividing the mean 50% cytotoxic concentration (CC<sub>50</sub>) for three human oral normal cells (gingival fibroblast, periodontal ligament fibroblast, pulp cell) (A) by that for four OSCC cells (Ca9-22, HSC-2, HSC-3, and HSC-4) (B) (T =A/B). …  PSE value that ​​reflects both tumor-specificity and cytotoxicity against cancer cells were calculated as follow: PSE= TS×100/B. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 3-P-351, 2020


  • Elucidation of neurite outgrowth by Wnt5a Ca<sup>2 +</sup>-dependently produced in periodontal ligament cells  [in Japanese]

    Takahashi Kaori , Yoshida Takashi , Wakamori Minoru

    … <p>Sensory nerves in the periodontal ligament (PDL) sense the occlusal pressure, and contribute to adjust the occlusal force. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 93(0), 1-SS-40, 2020


  • Effect of nicotine on periodontal ligament derived cells cultured under mechanical stress  [in Japanese]

    飯島 由貴

    歯科学報 119(6), 540-541, 2019-12-30


  • Role of intracellular Ca2+–based mechanotransduction of human periodontal ligament fibroblasts

    Hlaing Ei Ei Hsu , Ishihara Yoshihito , Wang Ziyi , Odagaki Naoya , Kamioka Hiroshi

    … Human periodontal ligament (hPDL) fibroblasts are thought to receive mechanical stress (MS) produced by orthodontic tooth movement, thereby regulating alveolar bone remodeling. … Compressive MS activated this autonomous responsive behavior with an increased percentage of responsive cells both in vitro and ex vivo . … Role of intracellular Ca2+‐based mechanotransduction of human periodontal ligament fibroblasts. …

    FASEB Journal 33(9), 10409-10424, 2019-09


  • A Human Amelogenin-Derived Oligopeptide Enhances Osteogenic Differentiation of Human Periodontal Ligament Stem Cells

    Takeuchi Tomoki , Masuno Kazuya , Kato Hirohito , Taguchi Yoichiro , Umeda Makoto , Okusa Nobutaka , Tanaka Akio , Tominaga Kazuya

    Journal of hard tissue biology 28(3), 251-257, 2019-07

  • Titanium dioxide-based scanning powder can modulate cell activity of oral soft tissue - Insights from in vitro studies with L929 cells and periodontal fibroblasts

    Oberoi Gunpreet , Müller Anna , Moritz Andreas , Shokoohi-Tabrizi Hassan Ali , Kurzmann Christoph , Agis Hermann

    … Murine L929 cells and human periodontal ligament (PDLF) and gingival fibroblasts (GF) were treated with ten-fold serial dilutions of scanning powder and the corresponding conditioned medium (filtrate of overnight incubation of powder in medium) starting with 30 mg/ml. … Formazan and resazurin conversion was lesser in L929 cells than PDLF and GF in the presence of scanning powder. …

    Journal of Prosthodontic Research 64(1), 34-42, 2019


  • Periodontal regenerative medicine using mesenchymal stem cells and biomaterials: A systematic review of pre-clinical studies

    PORTRON Sophie , SOUEIDAN Assem , MARSDEN Anne-Claire , RAKIC Mia , VERNER Christian , WEISS Pierre , BADRAN Zahi , STRUILLOU Xavier

    … <p>The aim of the systematic review was to analyze the use of mesenchymal stem cells (MSC) and biomaterial for periodontal regeneration from preclinical animal models and human. … These results indicate that MSC and scaffold provide beneficial effects on periodontal regeneration, with no adverse effects of such interventions. …

    Dental Materials Journal 38(6), 867-883, 2019


  • A Human Amelogenin-Derived Oligopeptide Enhances Osteogenic Differentiation of Human Periodontal Ligament Stem Cells

    Takeuchi Tomoki , Masuno Kazuya , Kato Hirohito , Taguchi Yoichiro , Umeda Makoto , Okusa Nobutaka , Tanaka Akio , Tominaga Kazuya

    … for periodontal tissue regeneration therapies in our previous study. … In the present study, we investigated the effect of a synthetic human amelogenin-derived peptide (HAP) consisting of WYQSIR on human periodontal ligament stem cells (PDLSCs) to be useful or not for the tissue regeneration. … The number of viable cells was significantly increased in the presence of HAP in normal medium. …

    Journal of Hard Tissue Biology 28(3), 251-258, 2019


  • MicroRNA expression profiling of nicotine-treated human periodontal ligament cells

    Du Anqing , Cheng Yawei , Zhao Sen , Wei Xiaoxia , Zhou Yi

    … <p>Cigarette smoking is a lifestyle-related risk factor involved in the causation and progression of periodontal disease. … The present study investigated the microRNA (miRNA) expression profile of human periodontal ligament cells (PDLCs) treated with nicotine. …

    Journal of Oral Science 61(2), 206-212, 2019

    J-STAGE  Ichushi Web 

  • Induction of chondrogenic or mesenchymal stem cells from human periodontal ligament cells through inhibition of Twist2 or Klf12

    Takai Hideki , van Wijnen Andre J. , Ogata Yorimasa

    … <p>Periodontitis leads to destruction of periodontal ligament, cementum and alveolar bone. … Regeneration of periodontal tissue is dependent on mesenchymal stem cells (MSC) present in the periodontal ligament, and transcription factors determine the direction of MSC differentiation. …

    Journal of Oral Science 61(2), 313-320, 2019

    J-STAGE  Ichushi Web 

  • Titanium dioxide nanoparticles induce COX-2 expression through ROS generation in human periodontal ligament cells

    Kim Do-Hee , Kundu Juthika , Chae In Gyeong , Lee Jong Kwon , Heo Jung Sun , Chun Kyung-Soo

    … Here, we assessed whether TiO<sub>2</sub>-NPs induce cyclooxygenase-2 (COX-2) and investigated the molecular mechanisms underlying the pro-inflammatory effect of TiO<sub>2</sub>-NPs on human periodontal ligament (PDL) cells. … Treatment of PDL cells with TiO<sub>2</sub>-NPs led to induction of both COX-2 mRNA and protein expression. …

    The Journal of Toxicological Sciences 44(5), 335-345, 2019

    J-STAGE  Ichushi Web 

  • Promotion of Tissue Healing and Regeneration with Low-Reactive Level Laser Therapy Using Diode Lasers in Dental Field: Review of Basic and Clinical Research  [in Japanese]

    Mikami Risako , Mizutani Koji , Aoki Akira , Noda Masahiro , Ejiri Kenichiro , Izumi Yuichi

    <p>1960年代以降,低出力レーザー照射(Low-reactive level laser/light therapy: LLLT)による生体賦活作用について基礎研究および臨床応用が進められてきた.中でも組織浸透性が高い近赤外から赤外領域の半導体レーザーを用いた研究は数多くなされており,歯科領域においても<i>in vitro</i>では歯肉線維芽細胞,骨芽細 …

    Nippon Laser Igakkaishi 40(1), 15-29, 2019


  • The Role of Low-Level Sodium Fluoride in Periodontal Inflammation

    Kim Il-Shin , Song Wenqun , Arakawa Hirohisa

    … In this study, we investigated the molecular basis for the down-regulation of toll-like receptor 4 (TLR4) signal transduction by low-level fluoride (NaF) in periodontal ligament cells. … In addition, the expression of asporin was investigated by quantitative real-time PCR as well as by immunohistochemical analysis of periodontal ligament cells, with or without fluoride treatment. …

    Journal of Hard Tissue Biology 28(2), 159-164, 2019

    J-STAGE  Ichushi Web 

  • Susceptibility of the Wnt/β-catenin Pathway Accelerates Osteogenic Differentiation of Human Periodontal Ligament Stem Cell Spheroids

    Toshimitsu Takuya , Ohno Jun , Kajiya Hiroshi , Yasunaga Madoka , Maeshiba Munehisa , Fujisaki Seiichi , Miyaguchi Naoyuki , Yamaguchi Masahiro , Maeda Hidefumi , Kojima Hiroshi

    … <p>Human periodontal ligament stem cells (HPLSCs) contribute to the regeneration of periodontal tissue because of their multilineage potential. … Increased expression levels of osterix and Runx2 and intense staining of alkaline phosphatase (ALP) activity were observed in spheroid-derived HPLSCs, as compared with monolayer-derived cells. …

    Journal of Hard Tissue Biology 28(2), 121-128, 2019


  • Heavily Induced Root Resorption Stimulates Wnt5a Expression in Cementoblasts

    Saito-Goto Hitomi , Kikuta Jun , Shimizu Mami , Hikida Takuji , Kasai Kazutaka

    … Additionally, the effects of different compression forces(CFs)on Wnt5a production were investigated using human periodontal ligament(hPDL) cells and human cementoblast-like cells in vitro. … Resorption lacunae with multinucleated cells in the CF-10 g group under experimental tooth movement conditions in vivo.Moreover, RANKL and Wnt5a immunoreactivity was detected in the alveolar bone-PDL tissues. …

    International Journal of Oral-Medical Sciences 17(3-4), 69-80, 2019

    J-STAGE  Ichushi Web 

  • Wnt5a Stimulates Bone Resorption during Orthodontic Tooth Movement

    Kikuta Jun , Saito-Goto Hitomi , Iwane Takehiro , Shimizu Mami , Hikida Takuji , Nakayama Erika , Kasai Kazutaka

    … The study then investigated the relation of compression force(CF)to Wnt5a expression from human periodontal ligament(PDL)cells.</p><p><b>Material and Methods </b>In vivo, to movement the maxillary first molar, we added 10 g of orthodontic force to twenty male rats for seven days. …

    International Journal of Oral-Medical Sciences 17(3-4), 62-68, 2019

    J-STAGE  Ichushi Web 

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