Search Results 1-20 of 3436

  • Expression of tartrate-resistant acid phosphatase and cathepsin K during osteoclast differentiation in developing mouse mandibles

    NAKAMURA Megumi , AOYAMA Naoki , YAMAGUCHI Satoshi , SASANO Yasuyuki

    … Von Kossa staining to determine when bone mineralization begins, tartrate-resistant acid phosphatase (TRAP) activity and cathepsin K immunoreactivity to identify the presence of osteoclasts, and their mRNA expression levels to assess osteoclastic differentiation in the embryonic mouse mandible. … Cathepsin K protein was expressed only in multinucleated osteoclasts, whereas TRAP activity was identified in both mono- and multinucleated cells. …

    Biomedical Research 42(1), 13-21, 2021

    J-STAGE 

  • Study of thermotolerant mechanism of Stropharia rugosoannulata under high temperature stress based on the transcriptome sequencing

    Ren Jifan , Wang Qingji , Zuo Jie , Jiang Shuxia

    … We find that the differential genes are mainly enriched in the pathways of glycerophospholipid metabolism, starch and sucrose metabolism, protein processing in the endoplasmic reticulum, etc. … The expression levels of genes encoding trehalose-6-phosphate phosphatase (TPP), CAT, SOD, etc. …

    Mycoscience, 2021

    J-STAGE 

  • Cancellation of the Calcification in Cultured Osteoblasts by CLEC-2

    Kanai Takenori , Sawa Yoshihiko , Sato Yoshiaki

    … However, the amounts of calcified nodules and alkaline phosphatase activity were significantly suppressed in cultured osteoblasts by CLEC-2. … The quantitative analysis showed that both the calcified nodule amount and alkaline phosphatase activity decreased with CLEC-2 while there was no influence in the cell viability with CLEC-2. …

    Journal of Hard Tissue Biology 30(1), 53-62, 2021

    J-STAGE 

  • JNK Pathway-Associated Phosphatase as a Serum Marker for Disease Activity and Treatment Outcome of Juvenile Idiopathic Arthritis

    Zhu Songbai , Lv Huijuan , Luo Yuanyuan , Huang Qing , Shen Jiangman

    … JNK pathway-associated phosphatase (JKAP) is reported to be a negative regulator of T-cell activation, but its clinical role in JIA is unknown. … Among JIA patients, higher JKAP levels were associated with lower disease activity indexes, including C-reactive protein, number of joints with active arthritis, physician's global assessment of disease activity, and the present history of disease-modifying antirheumatic drugs; …

    The Tohoku Journal of Experimental Medicine 253(1), 19-28, 2021

    J-STAGE 

  • Effect of aging on bone metabolism: the involvement of complemen t C1q

    Aonuma Fumiko , Higashi Sen , Tsuka Shintaro , Ohsumi Tomoko , Masaki Chihiro , Hosokawa Ryuji , Takeuchi Hiroshi

    … Osteogenic differentiation of MC3T3-E1 cells was assessed using alkaline phosphatase staining. … Differentiation of osteoclasts from mouse bone marrow cells was assessed using tartrate-resistant acid phosphatase staining. … Activation of canonical Wnt signaling and protein phosphorylation was monitored using Western blotting.<br><i>Results:</i> …

    Journal of Prosthodontic Research, 2021

    J-STAGE 

  • Pilea umbrosa ameliorate CCl4 induced hepatic injuries by regulating endoplasmic reticulum stress, pro-inflammatory and fibrosis genes in rat

    Naz Irum , Khan Muhammad Rashid , Zai Jawaid Ahmed , Batool Riffat , Zahra Zartash , Tahir Aemin

    Environmental Health and Preventive Medicine 25(1), 2020-09-11

    DOI 

  • The role of PTP1B in the regulation of glucose and energy metabolism  [in Japanese]

    坂野 僚一 , 杉山 摩利子 , 恒川 卓 , 伊藤 禎浩 , 有馬 寛

    糖尿病・内分泌代謝科 = Diabetology, endocrinology & metabolology 51(1), 36-41, 2020-07

    Ichushi Web 

  • Update of glucose and energy metabolism network  [in Japanese]

    山内 敏正

    糖尿病・内分泌代謝科 = Diabetology, endocrinology & metabolology 51(1), 1-3, 2020-07

  • Critical role of PP2A-B56 family protein degradation in HIV-1 Vif mediated G2 cell cycle arrest

    Nagata Kayoko , Shindo Keisuke , Matsui Yusuke , Shirakawa Kotaro , Takaori-Kondo Akifumi

    … Here we show that degradation of B56 family proteins (PP2A-B56), one of the regulatory subunits of protein phosphatase 2A, is critical for the Vif-induced G2 arrest. …

    Biochemical and Biophysical Research Communications 527(1), 257-263, 2020-06-18

    IR 

  • The fungal metabolite (+)-terrein abrogates osteoclast differentiation via suppression of the RANKL signaling pathway through NFATc1

    Nakagawa Saki , Omori Kazuhiro , Nakayama Masaaki , Mandai Hiroki , Yamamoto Satoshi , Kobayashi Hiroya , Sako Hidefumi , Sakaida Kyosuke , Yoshimura Hiroshi , Ishii Satoki , Ibaragi Soichiro , Hirai Kimito , Yamashiro Keisuke , Yamamoto Tadashi , Suga Seiji , Takashiba Shogo

    … RANKL-induced messenger RNA expression of osteoclast-specific markers including nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), the master regulator of osteoclastogenesis, cathepsin K, tartrate-resistant acid phosphatase (Trap) was completely inhibited by synthetic (+)-terrein treatment. … Furthermore, synthetic (+)-terrein decreased RANKL-induced NFATc1 protein expression. …

    International Immunopharmacology (83), 106429, 2020-06

    IR 

  • Hepatocyte ELOVL Fatty Acid Elongase 6 Determines Ceramide Acyl‐Chain Length and Hepatic Insulin Sensitivity in Mice

    松坂 賢 , 會田 雄一 , 岩﨑 仁 , 矢藤 繁 , 鈴木 浩明 , 武内 謙憲 , 矢作 直也 , 宮本 崇史 , 関谷 元博 , 高橋 智 , 島野 仁 , Takashi MATSUZAKA , Motoko Kuba , Saori Koyasu , Yuta Yamamoto , Kaori Motomura , Sundaram Arulmozhiraja , Hiroshi Ohno , Rahul Sharma , Takuya Shimura , Yuka Okajima , Song‐iee Han , Yuichi AITA , Yuhei Mizunoe , Yoshinori Osaki , Hitoshi IWASAKI , Shigeru YATOH , Hiroaki SUZUKI , Hirohito Sone , Yoshinori TAKEUCHI , Naoya YAHAGI , Takafumi MIYAMOTO , Motohiro SEKIYA , Yoshimi Nakagawa , Masatsugu Ema , Satoru TAKAHASHI , Hiroaki Tokiwa , Hitoshi SHIMANO

    … Hepatic patatin‐like phospholipase domain‐containing protein 3 (Pnpla3) expression was down‐regulated in LKO mice, and adenoviral Pnpla3 restoration reversed the enhancement in insulin sensitivity in HSD‐fed LKO mice. … Ceramide(d18:1/18:0) enhances protein phosphatase 2A (PP2A) activity by interfering with the binding of PP2A to inhibitor 2 of PP2A, leading to Akt dephosphorylation. …

    Hepatology 71(5), 1609-1625, 2020-05

    IR 

  • Detailed analyses of the crucial functions of Zn transporter proteins in alkaline phosphatase activation

    Suzuki Eisuke , Ogawa Namino , Takeda Taka-Aki , Nishito Yukina , Tanaka Yu-Ki , Fujiwara Takashi , Matsunaga Mayu , Ueda Sachiko , Kubo Naoya , Tsuji Tokuji , Fukunaka Ayako , Yamazaki Tomohiro , Taylor Kathryn M , Ogra Yasumitsu , Kambe Taiho

    … Here, using genetically-engineered chicken DT40 cells, we first determined that Zrt/Irt-like protein (ZIP) transporters that are localized to the compartments of the early secretory pathway play only a minor role in the ALP activation process. …

    Journal of biological chemistry 295(17), 5669-5684, 2020-04-24

    IR 

  • A STUDY OF URINARY EXOSOMES IN GENETICALLY DEFINED DENT'S DISEASE  [in Japanese]

    Inoue Masataka , Tamura Hiroaki , Takahashi Tsutomu

    … In Dent's disease, reabsorption of glomerulus-filtered LMW protein in the proximal tubular cells is decreased due to a malfunction of cellular endocytosis caused either by mutation in the Cl⁻/H⁺ exchanger ClC-5-encoding CLCN5 gene (in about 60% cases) or the phosphatidylinositol 4.5-biphosphate 5-phosphatase-encoding OCRL1 gene (in about 20% cases). …

    秋田医学 = AKITA JOURNAL OF MEDICINE(AKITA IGAKU) 46(3), 99-106, 2020-03-30

    IR  DOI 

  • Antibacterial and pro-osteogenic effects of β-Defensin-2-loaded mesoporous bioglass

    REN Le , ZHANG Zhe , DENG Chunni , ZHANG Nan , LI Daxu

    … in vitro</i>, and resulted in significantly lower amounts of viable bacteria and colony forming units, and significantly higher levels of bacterial protein release compared with those in the BC and MBG groups (all <i>p</i><0.05). …

    Dental Materials Journal, 2020

    J-STAGE 

  • Surface modification of titanium with collagen/hyaluronic acid and bone morphogenetic protein 2/7 heterodimer promotes osteoblastic differentiation

    WU Chengzhong , SHAO Xia , LIN Xianglin , GAO Weijin , FANG Yiming , WANG Jingxiao

    … <p>The aim of this study was to evaluate the effects of a collagen/hyaluronic acid coating without or with incorporated heterodimeric bone morphogenetic protein 2/7 (BMP2/7) on <i>in-vitro</i> … The effects of the coatings were evaluated by assessing the alkaline phosphatase (ALP) activity (an early osteoblastic differentiation marker) and the osteocalcin expression (a late osteoblastic differentiation marker). …

    Dental Materials Journal 39(6), 1072-1079, 2020

    J-STAGE 

  • Redox cycling of 9,10-phenanthrenequinone activates epidermal growth factor receptor signaling through <i>S</i>-oxidation of protein tyrosine phosphatase 1B

    Luong Nho Cong , Abiko Yumi , Shibata Takahiro , Uchida Koji , Warabi Eiji , Suzuki Midori , Noguchi Takuya , Matsuzawa Atsushi , Kumagai Yoshito

    <p>[Page 358, Figure 5C] The label of Western blot data in Figure 5C in the paper was erroneously placed.</p>

    The Journal of Toxicological Sciences 45(12), 807-807, 2020

    J-STAGE 

  • JTE-952 Suppresses Bone Destruction in Collagen-Induced Arthritis in Mice by Inhibiting Colony Stimulating Factor 1 Receptor

    Uesato Naofumi , Matsushita Mutsuyoshi , Inagaki Koji , Miyagawa Naoki , Kitagawa Yoshihiro , Kakefuda Reina , Matsuo Yushi , Yamaguchi Takayuki , Hata Takahiro , Ikegashira Kazutaka

    … Colony stimulating factor 1 receptor (CSF1R) is a receptor protein tyrosine kinase specifically expressed in monocytic-lineage cells such as macrophages and osteoclasts. … JTE-952 significantly suppressed increases in the bone destruction score, the number of tartrate-resistant-acid-phosphatase-positive cells, and the severity of arthritis in the model mice. …

    Biological and Pharmaceutical Bulletin 43(12), 1884-1892, 2020

    J-STAGE 

  • Identification of pyrogallol from Awa-tea as an anti-allergic compound that suppresses nasal symptoms and IL-9 gene expression

    Nakano Tomohiro , Fukui Hiroyuki , Takeda Noriaki , Mizuguchi Hiroyuki , Ikeda Mitsuhiro , Wakugawa Tomoharu , Kashiwada Yoshiki , Kaminuma Osamu , Kitamura Noriko , Yabumoto Masam , Fujino Hiromichi , Kitamura Yoshiaki

    … We demonstrated that protein kinase Cδ / heat shock protein 90-mediated H1R gene expression signaling and nuclear factor of activated T-cells (NFAT)-mediated IL-9 gene expression signaling are responsible for the pathogenesis of pollinosis. … Pyrogallol itself did not inhibit calcineurin phosphatase activity. …

    The Journal of Medical Investigation 67(3.4), 289-297, 2020

    IR  J-STAGE 

  • Two new missense mutations in the protein interaction ASH domain of OCRL1 identified in patients with Lowe syndrome

    Perdomo-Ramirez Ana , Antón-Gamero Montserrat , Sakaguchi Rizzo Daniela , Trindade Amelia , Ramos-Trujillo Elena , Claverie-Martin Felix

    … gene encoding membrane bound inositol polyphosphate 5-phosphatase OCRL1. … gene of two Lowe syndrome patients and report two new missense mutations that affect the ASH domain involved in protein-protein interactions. … We suggest that p.V636E and p.H660P cause Lowe syndrome by disrupting the interaction of OCRL1 with other proteins or by impairing protein stability.</p> …

    Intractable & Rare Diseases Research 9(4), 222-228, 2020

    J-STAGE 

  • Free Radical Production and Production Mechanism in the Early and Advanced Stages of Gastrointestinal Lesions  [in Japanese]

    Yasukawa Keiji

    … <p>Disruption of redox balance due to the overproduction of free radicals and reactive oxygen species (ROS) could cause protein denaturation, lipid peroxidation, and DNA mutation. … Furthermore, nitration of src homology protein tyrosine phosphatase 2 in macrophages might be involved in the activation of Toll-like receptor 4 and NF-κB, inducing infiltration of activated neutrophils into colonic mucosa to produce ROS in DSS-induced colitis mice.</p> …

    YAKUGAKU ZASSHI 140(11), 1343-1350, 2020

    J-STAGE 

Page Top