Search Results 1-20 of 2354

  • Efficient ssODN-Mediated Targeting by Avoiding Cellular Inhibitory RNAs through Precomplexed CRISPR-Cas9/sgRNA Ribonucleoprotein

    Kagita Akihiro , Lung Mandy S.Y. , Xu Huaigeng , Kita Yuto , Sasakawa Noriko , Iguchi Takahiro , Ono Miyuki , Wang Xiou H. , Gee Peter , Hotta Akitsu

    CRISPR-Cas9がヒト細胞内のRNAで阻害されてしまう現象を発見し、iPS細胞での効率的な相同組み換えゲノム編集技術を実現. 京都大学プレスリリース. 2021-03-12.A simple step to enhance CRISPR-Cas9 genome editing. 京都大学プレスリリース. 2021-03-12.

    Stem Cell Reports, 2021-03-11


  • Dynamics of HMGB1 in vascular endothelial cells and tumor vessels-targeting therapy using conjugated anti-HMGB1 mAb for cancers  [in Japanese]

    Nishibori Masahiro , Gao Shangze , Tomonobu Naoko , Sakaguchi Masakiyo

    … <p>High mobility group box-1 (HMGB1), originally identified as a nuclear chromatin DNA-binding protein, plays a very important role in triggering the inflammatory responses in many kids of diseases once released into extracellular space. … HMGB1 directly stimulates RAGE and TLR-4/2, and enhances the signal transduction by forming complexes with CXCL12 and IL-1beta. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-S26-3, 2021


  • Novel physiological functions of free fatty acid receptors  [in Japanese]

    Ichimura Atsuhiko

    … A strategy to deorphanize G-protein-coupled receptors (GPCRs) previously identified a series of receptors, which are activated by FFAs. … In this symposium, I will introduce recent advances on the key physiological functions of the FFARs-mediated signaling transduction pathways in the regulation of metabolism and immune responses. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-S25-3, 2021


  • Single-molecule imaging of GPCR signalosome in living cell  [in Japanese]

    Yanagawa Masataka

    … <p>G protein-coupled receptors (GPCRs) act as a signaling hub through G proteins and arrestins upon ligand binding. … The NanoBiT assays and BRET imaging revealed the AT1R/G protein/GRK preassembly complex in living cell membrane. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-S25-2, 2021


  • Collapsin response mediator protein 2 was one of targets for 15-deoxy -Δ<sup>12,14</sup>-prostaglandin J<sub>2</sub>  [in Japanese]

    Yagami Tatsurou , Yamamoto Yasuhiro , Koma Hiromi

    … (15d-PGJ<sub>2</sub>) is one of factors contributed to the neurotoxicity of amyloid β, a causative protein of Alzheimer's disease. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-P1-34, 2021


  • Hsp90 contributes to cardiac hypertrophy in the development of heart failure via regulating c-Raf.  [in Japanese]

    Itoh Takumi , Marunouchi Tetsuro , Yano Emi , Tanonaka Kouichi

    … Hsp90 client proteins are diverse and contain many signal transmitters including protein kinases. … Cardiac remodeling is regulated by many signaling pathways, and c-Raf that is also an Hsp90 client protein is a signaling molecule involved in cardiac hypertrophy. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-P2-08, 2021


  • Analyses of Forming Hetero-oligomers and Regulating Signal transductions of G-Protein Coupled Receptors (GPCRs) Expressed in the Central Nervous System.  [in Japanese]

    Sakairi Hakushun , Kamikubo Yuji , Kono Hikaru , Yamamoto Kei , Tabata Toshihide , Sakurai Takashi

    … <p>G-Protein coupled receptors (GPCRs) form the largest family of cell surface receptor proteins and play key roles in eukaryotic cells, including in neuron. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 3-O-B1-1, 2021


  • Role of alpha 1 antitrypsin-induced ER stress in placental trophoblast cells  [in Japanese]

    Yoshida Kanoko , Kusama Kazuya , Kato Kiyoko , Ishikawa Gen , Yoshie Mikihiro , Tamura Kazuhiro

    … Several reports have identified A1AT as a hypertensive disorders of pregnancy (HDP) or preeclampsia-related protein. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 2-Y-E2-2, 2021


  • Regulation of receptor chaperone molecule RTP4 in neuronal cells by opioid receptor activation  [in Japanese]

    Fujita Wakako , Aruga Jun

    … <p>Receptor transporter protein family members (RTP1-4) are known to promote the trafficking of G-protein coupled receptor to the cell surface membrane. … Together, these results suggest the expression of RTP4 is upregulated by MOPr activation via G-protein, MEK and/or JAK mediated pathways, and this, in turn, is likely to enhance the cell surface expression of MOPr-DOPr heteromers.</p> …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 2-P1-19, 2021


  • A modeling of environmental electrophile exposome  [in Japanese]

    Kumagai Yoshito

    … Such reactive substances are reported to covalently modify protein thiols, resulting in formation of protein adducts. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 1-S10-1, 2021


  • Glycyrrhizin ameliorates melanoma cell extravasation into mouse lungs by regulating signal transduction through HMGB1 and its receptors

    Hiramoto Keiichi , Yamate Yurika , Goto Kenji , Ohnishi Shiho , Morita Akihiro , Yoshikawa Nobuji , Kawanishi Shosuke

    … Furthermore, glycyrrhizin ameliorated the lung tissue expression of high mobility group box-1 protein (HMGB1), receptor for advanced glycation end products (RAGE), Toll-like receptor (TLR)-4, RAS, extracellular signal-related kinase, NF-κB, myeloid differentiation primary response 88, IκB kinase complex, epithelial-mesenchymal transition markers, and vascular endothelial growth factor-A. …

    Journal of Clinical Biochemistry and Nutrition, 2021


  • Evaluation of Combinatory Effects of <i>Plasmodium</i> Circumsporozoite Protein and Complement Regulatory Protein Expression of Recombinant Baculovirus Vectors

    Kawabata Chiaki , Kawai Yusuke , Tamura Takahiko

    … However, the transduction activity of BVs is strongly reduced by the attack of serum complement, which is one of the major obstacles in the use of BVs for <i>in vivo</i> … circumsporozoite protein (CSP) to enhance transduction efficacy in hepatic cells. … In this study, we investigated the combination of CSP and CRPs in a BV system to evaluate transduction efficacy along with complement resistance. …

    Biological and Pharmaceutical Bulletin 44(2), 219-224, 2021


  • Functional Alterations of Multidrug Resistance-Associated Proteins 2 and 5, and Breast Cancer Resistance Protein upon Snail-Induced Epithelial–Mesenchymal Transition in HCC827 Cells

    Yano Kentaro , Todokoro Itsuki , Kamioka Hiroki , Tomono Takumi , Ogihara Takuo

    … Our objective is to evaluate the changes in the mRNA and protein expression levels and the functions of multidrug resistance-associated protein (MRP) 2, MRP5 and breast cancer resistance protein (BCRP). … Snail-expressing HCC827 cells showed increased mRNA levels of Snail and a mesenchymal marker vimentin, and decreased mRNA levels of an epithelial marker E-cadherin after transduction, indicating that Snail had induced EMT consistent with our previous reports. …

    Biological and Pharmaceutical Bulletin 44(1), 103-111, 2021


  • Antioxidative and anti-inflammatory actions of reactive cysteine persulfides

    Zhang Tianli , Tsutsuki Hiroyasu , Ono Katushiko , Akaike Takaaki , Sawa Tomohiro

    … Recent advances in the development of analytical methods for detection and quantification of persulfides and polysulfides have revealed the biological presence, in both prokaryotes and eukaryotes, of persulfide/polysulfide in diverse forms such as CysSSH, glutathione persulfide and protein persulfides. …

    Journal of Clinical Biochemistry and Nutrition 68(1), 5-8, 2021


  • High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases

    Ikuko Koyama-Honda , Takahiro K. Fujiwara , Rinshi S. Kasai , Kenichi G.N. Suzuki , Eriko Kajikawa , Hisae Tsuboi , Taka A. Tsunoyama , Akihiro Kusumi

    … The recruitment depended on the PM cholesterol and saturated alkyl chains of Lyn and H-Ras, whereas it was blocked by the nonraftophilic transmembrane protein moiety and unsaturated alkyl chains linked to the inner-leaflet molecules. …

    Journal of Cell Biology 219(12), e202006125, 2020-12-07


  • Antagonistic regulation of the gibberellic acid response during stem growth in rice

    Nagai Keisuke , Mori Yoshinao , Ishikawa Shin , Furuta Tomoyuki , Gamuyao Rico , Niimi Yoko , Hobo Tokunori , Fukuda Moyuri , Kojima Mikiko , Takebayashi Yumiko , Fukushima Atsushi , Himuro Yasuyo , Kobayashi Masatomo , Ackley Wataru , Hisano Hiroshi , Sato Kazuhiro , Yoshida Aya , Wu Jianzhong , Sakakibara Hitoshi , Sato Yutaka , Tsuji Hiroyuki , Akagi Takashi , Ashikari Motoyuki


    Nature 584(7819), 109-114, 2020-08-06


  • Ligation of MHC Class II Induces PKC-Dependent Clathrin-Mediated Endocytosis of MHC Class II

    Masaki Kento , Hiraki Yuhji , Onishi Hiroka , Satoh Yuka , Roche Paul A. , Tanaka Satoshi , Furuta Kazuyuki

    … In addition to antigen presentation to CD4(+)T cells, aggregation of cell surface major histocompatibility complex class II (MHC-II) molecules induces signal transduction in antigen presenting cells that regulate cellular functions. … Treatments with a phorbol ester promoted MHC-II endocytosis, whereas inhibitors of protein kinase C (PKC) suppressed crosslinking-induced endocytosis of MHC-II. …

    Cells 9(8), 1810, 2020-07-30


  • Research on the Mitochondrial Zone Responsible for Innate Immunity  [in Japanese]

    錦織 充広 , 小柴 琢己

    … The mitochondrion-related innate immune response is accomplished by the activation of two distinct signaling transduction pathways, that are the retinoic acid-inducible gene I (RIG-I) pathway and the NLR family pyrin domain-containing 3 (NLRP3) inflammasomes. … In both signaling pathways, a mitochondrial outer membrane adaptor protein, mitochondrial antiviral signaling (MAVS), acts as a key role in the signal transduction. …

    福岡医学雑誌 = Fukuoka acta medica 111(2), 77-85, 2020-06-25

    IR  DOI  Ichushi Web 

  • Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein

    Shinohara Takashi , Kanatsu-Shinohara Mito

    … Here, we show efficient gene transfer into SSCs via a lentivirus (FV-LV) using a fusion protein (F), a Sendai virus (SV) envelope protein involved in virion/cell membrane fusion. … In vivo microinjection showed that FV-LVs could penetrate not only the basement membrane of the seminiferous tubules but also the blood-testis barrier, which resulted in successful transduction of both spermatogenic cells and testicular somatic cells. …

    Stem Cell Reports 14(3), 447-461, 2020-03-10


  • IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95

    Kim Hyunsoo , Takegahara Noriko , Walsh Matthew C. , Middleton Sarah A. , Yu Jiyeon , Shirakawa Jumpei , Ueda Jun , Fujihara Yoshitaka , Ikawa Masahito , Ishii Masaru , Kim Junhyong , Choi Yongwon

    … IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95. … Here, we demonstrate that immunoglobulin superfamily member 11 (IgSF11), whose expression increases during osteoclast differentiation, regulates osteoclast differentiation through interaction with postsynaptic density protein 95 (PSD-95), a scaffold protein with multiple protein interaction domains. …

    Bone Research (8), 5, 2020-02-10


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