Search Results 1-20 of 12470

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A Strategy for Protein Identification by Using Spectral Libraries of Chemically Modified Trypsin Autolysis Peptide Fragments [in Japanese]

Yoshino Ken-ichi , Usami Itaru , Takeuchi Atsuko

… Chemically modified trypsin by reductive methylation is a standard reagent for identifying proteins by mass spectrometry. …

Journal of the Mass Spectrometry Society of Japan 69(1), 8-19, 2021

J-STAGE
2

Development of Precolumn Derivatization–LC/MS for Amino-Acid-Focused Metabolomics

MIYANO Hiroshi , NAKAYAMA Akira

… We also developed an automated precolumn derivatization amino acid analyzer using another reagent for liquid chromatography–mass spectrometry (LC/MS). …

CHROMATOGRAPHY, 2021

J-STAGE
3

Studies on Functional Molecules Based on Peptide Chemistry [in Japanese]

Akaji Kenichi

… Chloroimidazolidium coupling reagent developed by us was effective for the successive couplings of the α-methyl cysteine residues. …

YAKUGAKU ZASSHI 141(2), 215-233, 2021

J-STAGE
4

Evaluation of test performance of Simon's reagent and Marquis reagent for so-called Ecstasy tablets [in Japanese]

Tsujikawa Kenji , Okada Yuki , Segawa Hiroki , Yamamuro Tadashi , Kuwayama Kenji , Kanamori Tatsuyuki , Iwata Yuko T. , Inoue Hiroyuki

… A combination of Simon's reagent and Marquis reagent has been used as a field test for Ecstasy tablets in Japan. … When 1 mg of the mixture of MDMA hydrochloride and the diluent was used, the lowest MDMA hydrochloride concentration giving positive was 25%, except when the combination of Simon's reagent and the mixture of MDMA hydrochloride and cellulose (1%) was used. …

Japanese Journal of Forensic Science and Technology 26(1), 103-113, 2021

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5

Study of male DNA quantification methods in male-female mixture samples [in Japanese]

Tanaka Sachi , Ohta Jun , Yamazaki Kazuki

… For more efficient testing Y-STR type, we examined the forensic evaluation (sensitivity, repeatability, specificity, inhibition, comparison with commercial qPCR reagent and simulated mixture study) of qPCR assays targeting Y-chromosomal Sex determining region Y (SRY) and DYZ5 regions. … The SRY assay had the same sensitivity as the commercial qPCR reagent, but had more resistant to inhibitors. …

Japanese Journal of Forensic Science and Technology, 2021

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6

Inactivation of SARS-CoV-2 by Catechins from Green Tea

Nishimura Hidekazu , Okamoto Michiko , Dapat Isolde , Katumi Masanori , Oshitani Hitoshi

… Serially 10-fold diluted solutions of catechin mixture reagent from green tea were mixed with the viral culture fluid at a volume ratio of nine to one, respectively, and kept at room temperature for 5 min. The solution of 10 mg/mL catechin reagent reduced the viral titer by 4.2 log and 1.0 mg/mL solution reduced only by one log. … Pre-infection treatment of the cells with the reagent alone did not affect the viral growth. …

Japanese Journal of Infectious Diseases, 2021

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7

Fundamental study of the reagent "Fungitec G test ES Nissui" for (1→3)β-D glucan assay [in Japanese]

KOBAYASHI Ryo , NIRASAWA Shinya , SATO Yuki , ASANUMA Kouichi , YANAGIHARA Nozomi , TAKAHASHI Satoshi

(1→3)β-Dグルカン（BG）は深在性真菌症の血清学的診断補助マーカーとして汎用されている。その測定において，従来用いられてきた比濁法は測定時間が長く，比色法はバッチ測定のため検体の追加測定が困難であることから，1日の測定回数に制限があった。近年，比色法のリアルタイム処理試薬である「ファンギテック® GテストES「ニッスイ」」（ニッ …

Japanese Journal of Medical Technology 70(1), 93-98, 2021

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8

Stability evaluation of wet and dry reagents under harsh environmental conditions assuming three days until power restoration by utilizing VITROS XT7600 [in Japanese]

KIKUCHI Ryosuke , MATSUYAMA Hiroyuki , WATARAI Rika , YOKOYAMA Satoru , SUZUKI Atsuo , ANDO Yoshitaka , MATSUSHITA Tadashi

VITROS XT7600（XT7600）は無給水で分析が可能な自動分析装置であり，ビトロスマイクロスライド（ドライ）試薬に加え，汎用液状（ウェット）試薬をオープンチャンネルに搭載する運用での測定も可能である。本研究では，事業継続計画に基づく3日間の停電を想定し，試薬保管温度によるウェット試薬とドライ試薬の安定性への影響を検証した。測定機器はXT7600と対照機器としてLABOS …

Japanese Journal of Medical Technology 70(1), 86-92, 2021

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9

Performance evaluation of highly sensitive rapid test system for influenza virus antigen using SPOTCHEM FLORA SF-5520 and SPOTCHEM FLORA FluAB [in Japanese]

IKEUCHI Ayaka , NAKANISHI Kayoko , NISHIYAMA Yukiko , NAGAO Miki

アークレイ株式会社より発売されている，ユーロピウム（以下Eu）蛍光ラテックスを用いたインフルエンザウイルス抗原検出キット「スポットケムFLORA FluAB」は，時間分解蛍光検出法（以下TRF法）という新しいシグナル検出法を採用した専用の自動判定免疫蛍光分析装置「スポットケムFLORA」を用いて測定を行う。今回，複数の測定スロットで3検体まで並列処理が可能なスポットケムFLORA …

Japanese Journal of Medical Technology 70(1), 116-122, 2021

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10

Basic study of Zn measurement using the reagent 'Espa-Zn II': Performance evaluation of zinc measurement reagent [in Japanese]

TATEISHI Wataru , UKEDA Kaname , MACHIDA Satoshi , TSUBOI Isami

当社では亜鉛測定を原子吸光分光光度法にて行っているが，酢酸亜鉛製剤の適用拡大に伴い検体数が増え，対応法を検討する必要がある。そこで，検体の処理能力の高い汎用生化学自動分析機用の比色法を測定原理とした「エスパ・Zn II」の基礎的検討を行い，日常の検査に適用可能か確認した。その結果，再現性（同時再現性および日差再現性）は変動係数3.0%以内，検出限界は4.19 μg/dL，希釈直線 …

Japanese Journal of Medical Technology 70(1), 80-85, 2021

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11

Evaluation of a new procalcitonin assay kit and reagents, and clinical usefulness of procalcitonin [in Japanese]

TAKEBUCHI Mayu , USAMI Yoko , OGINO Yuika , ENDO Risa , MATSUMOTO Go , ISHIMINE Nau , SUGANO Mitsutoshi , HONDA Takayuki

【背景】プロカルシトニン（PCT）は敗血症マーカーとして利用されており，ブラームス社の抗体を使用した測定試薬が発売されている。新たに富士フイルム和光純薬株式会社から独自の抗PCT抗体を用いた定量試薬「ミュータスワコー PCT」（μTAS）と「アキュラシードPCT」（Accuraseed），イムノクロマト法を原理とする半定量試薬「スムーズチェックワコーPCT」（Smooth Che …

Japanese Journal of Medical Technology 70(1), 66-73, 2021

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12

Properties of Magnesium Electrode Covered with Magnesium Chloride-Modified Graphene Oxide

EGASHIRA Minato , HIRATSUKA Kaori

… For the modification of reversibility of magnesium deposition–dissolution in non-aqueous glyme-based electrolytes, the surface of a magnesium metal or magnesium alloy AZ31 was covered by graphene oxide modified using Grignard reagent. …

Electrochemistry 89(1), 49-53, 2021

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13

Design of Functional Nanoparticles for Intractable Disease Therapy

Koide Hiroyuki

… thus, they are of significant interest as a cost-effective protein affinity reagent. …

Biological and Pharmaceutical Bulletin 44(1), 1-6, 2021

J-STAGE
14

Removal of Residual Chemical Reagent in The Cell Culture Environment [in Japanese]

柿本隆志 , 阿部公揮 , 冨田賢吾 , 田中勲 , 栗原隆

清水建設研究報告 98, 143-150, 2020-12
15

Evaluation of BioPlex 2200 Vasculitis Reagent Pack Simultaneously Detecting MPO-ANCA, PR3-ANCA, and Anti-GBM Antibodies in Patients with ANCA-associated Vasculitis [in Japanese]

柴田美帆 , 齋藤敏晴 , 森あやの , 髙橋未帆 , 林秀敏 , 松田武史 , 天野典彦 , 吉田克之 , 髙橋宗史 , 籏智さおり , 熊谷俊一

臨床病理 = The official journal of Japanese Society of Laboratory Medicine : 日本臨床検査医学会誌 68(11), 889-898, 2020-11
16

Influence of Autoantibodies to Prothrombin on the Newly Developed Thrombin-Antithrombin Complex Measuring Reagent Using Latex Turbidimetric Immunoassay [in Japanese]

吉田竜也 , 吉田美香 , 内藤澄悦 , 大村一将 , 高橋伸彦 , 家子正裕

臨床病理 = The official journal of Japanese Society of Laboratory Medicine : 日本臨床検査医学会誌 68(11), 877-882, 2020-11
17

Fundamental evaluation of Magnesium measuring reagent [in Japanese]

吉春奈緒 , 福間弘行 , 田村克実 , 糸賀真理

島根医学検査 = The Shimane journal of medical technology : (一社)島根県臨床検査技師会会誌 48(1), 10-14, 2020-08
18

Quantitative reagent of IgG4 [in Japanese]

菅野光俊 , 上原剛

臨床検査 = Journal of clinical laboratory medicine 64(8), 900-906, 2020-08

Ichushi Web
19

Neurosurgery for brain metastasis from breast cancer

Tomita Yusuke , Kurozumi Kazuhiko , Fujii Kentaro , Shimazu Yosuke , Date Isao

… Additionally, 5-aminolevulinic acid (5-ALA) is a promising reagent for photodynamic detection of residual tumor tissue. …

Translational Cancer Research 9(8), 5063-5076, 2020-08

IR
20

A selective and highly sensitive high performance liquid chromatography with fluorescence derivatization approach based on Sonogashira coupling reaction for determination of ethinyl estradiol in river water samples

Ali Marwa F.B. , Uejo Yurina , Kishikawa Naoya , Ohyama Kaname , Kuroda Naotaka

… The fluorescence derivatization procedure was based on Sonogashira coupling reaction using 4-(4, 5-diphenyl-1H-imidazole-2-yl) iodobenzene (DIB-I), a fluorescence labeling reagent, to derivatize EE in presence of copper and palladium ions. …

Journal of Chromatography A (1628), 461440, 2020-07-29

IR

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