Search Results 1-20 of 624

  • Genome-wide analysis of DNA methylation identifies apoptosis-related gene UQCRH in renal cancer  [in Japanese]

    Shogo Ehata , Miyakuni Kosuke , Nishida Jun , Miyazono Kohei

    … DNA methylated sites were genome-widely analyzed using methylation array in reference to RNA-sequencing data. …

    Proceedings for Annual Meeting of The Japanese Pharmacological Society 94(0), 2-P2-42, 2021

    J-STAGE 

  • <i>ErbB2</i> copy number gain is associated with adverse outcome in canine mammary carcinoma

    SAKAI Kosei , CHAMBERS James Ken , UCHIDA Kazuyuki , NAKAGAWA Takayuki , NISHIMURA Ryohei , YONEZAWA Tomohiro , MAEDA Shingo

    … copy number in these samples was determined as compared to a reference gene on canine chromosome 8. …

    Journal of Veterinary Medical Science 83(3), 370-377, 2021

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  • Identification and physiological function of one microRNA (<i>Po-MilR-1</i>) in oyster mushroom <i>Pleurotus ostreatus</i>

    Xu Danyun , Zhou Qixia , Yan Biyun , Ma Aimin

    … <p>MicroRNAs are essential regulators of gene expression and have been extensively studied in plants and animals; … This study explored the development-related function of a mushroom microRNA and will provide a reference for other microRNAs.</p> …

    Mycoscience, 2021

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  • Selection of reference genes for quantitative PCR analysis in poultry red mite (<i>Dermanyssus gallinae</i>)

    ARIIZUMI Takuma , KONNAI Satoru , OHASHI Kazuhiko , MURATA Shiro , FUJISAWA Sotaro , ISEZAKI Masayoshi , MAEKAWA Naoya , OKAGAWA Tomohiro , SATO Takumi , OISHI Eiji , TANENO Akira

    … However, reference genes are preferable for accurate comparison of expression level changes given the large variation in the quality of the PRM samples collected in each farm. … This study aimed to identify candidate reference genes with stable expression levels in the different blood feeding states and life stages of PRMs. … We selected and characterized seven candidate reference genes. …

    Journal of Veterinary Medical Science, 2021

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  • DNA Markers and Molecular Breeding in Pear and Other Rosaceae Fruit Trees

    Yamamoto Toshiya

    Reference genetic linkage maps and genome-wide molecular markers have enabled practical marker-assisted selection for resistance to black spot and/or pear scab diseases, self-compatibility, harvest time, and fruit skin color in Japanese pear breeding programs. … Borkh), and have been used to efficiently predict the function of a gene of interest and develop selection markers in related species.</p> …

    The Horticulture Journal 90(1), 1-13, 2021

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  • Identification of specific serotypes of fowl adenoviruses isolated from diseased chickens by PCR

    MASE Masaji , HIRAMATSU Kanae , NISHIJIMA Noriko , ISEKI Hiroshi , WATANABE Satoko

    … primers were designed based on serotype-specific sequences of the hexon gene. … We tested field isolates from chickens diagnosed with inclusion body hepatitis, gizzard erosion and hydropericardium syndrome together with reference FAdV strains characterized in Japan. …

    Journal of Veterinary Medical Science 83(1), 130-133, 2021

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  • Genome analyses reveal the hybrid origin of the staple crop white Guinea yam (Dioscorea rotundata)

    Sugihara Yu , Darkwa Kwabena , Yaegashi Hiroki , Natsume Satoshi , Shimizu Motoki , Abe Akira , Hirabuchi Akiko , Ito Kazue , Oikawa Kaori , Tamiru-Oli Muluneh , Ohta Atsushi , Matsumoto Ryo , Agre Paterne , De Koeyer David , Pachakkil Babil , Yamanaka Shinsuke , Muranaka Satoru , Takagi Hiroko , White Ben , Asiedu Robert , Innan Hideki , Asfaw Asrat , Adebola Patrick , Terauchi Ryohei

    西アフリカの主食作物ギニアヤムの起源を解明 --ギニアヤムはサバンナと熱帯雨林に生育する野生種の雑種起源--. 京都大学プレスリリース. 2020-12-11.

    Proceedings of the National Academy of Sciences of the United States of America 117(50), 31987-31992, 2020-12-15

    IR 

  • Molecular signatures of the rediae, cercariae and adult stages in the complex lifecycles of parasitic flatworms (Digenea: Psilostomatidae)

    Maksim A. Nesterenko , Viktor V. Starunov , Sergei V. Shchenkov , Anna R. Maslova , Sofia A. Denisova , Andrey I. Granovich , Andrey A. Dobrovolskij , Konstantin V. Khalturin

    … High-quality transcriptomes were generated, and the reference sets of protein-coding genes were used for differential expression analysis in order to identify stage-specific genes. … Comparative analysis of gene sets, their expression dynamics and Gene Ontology enrichment analysis were performed for three life stages within each species and between the two species.RESULTS: Reference transcriptomes for P. …

    Parasites & Vectors 13(1), 559, 2020-11-10

    IR 

  • Correlation between Organelle Genetic Variation and RNA Editing in Dinoflagellates Associated with the Coral Acropora digitifera

    Eiichi Shoguchi , Yuki Yoshioka , Chuya Shinzato , Asuka Arimoto , Debashish Bhattacharya , Noriyuki Satoh

    … digitifera, and by mapping reads to the available reference genome sequences, we extracted 2,250 sequences representing 15 organelle genes of Symbiodiniaceae. … Molecular phylogenetic analyses of these mitochondrial and plastid gene sets revealed that A. …

    Genome Biology and Evolution 12(3), 203-209, 2020-02-27

    IR 

  • A Study of Frequency Dependency of Cellular Deformation for a Mechanosensing Mechanism of a Cultured Cell under Mechanical Vibration  [in Japanese]

    SATO Katsuya , SHIRAISHI Toshihiko

    … One of the previous studies culturing osteoblastic cells under mechanical vibration reported that gene expression levels related to bone formation reached maximum dependent of frequencies like a resonance curve from a mechanical engineering point of view. … To measure the displacement from the images captured under static state and dynamic state where the velocity of vibration was 0, fluorescent microspheres were adhered on the bottom of a cell culture dish as the reference positions. …

    The Proceedings of the Dynamics & Design Conference 2020(0), 408, 2020

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  • A Graph Genome Browser: Visualization of Structural Variations between Genomes using a Graph Structure  [in Japanese]

    T. YOKOYAMA Toshiyuki , SAKAMOTO Yoshitaka , SEKI Masahide , SUZUKI Yutaka , KASAHARA Masahiro

    <p>ゲノムの個体間の差異に着目し、数学的なグラフ構造としてゲノムを可視化するためのツールであるグラフゲノムブラウザを開発した。ゲノム科学の研究において、遺伝情報の総体であるゲノムを可視化することは、その差異を検証し、解釈する上で重要である。このとき、複数のゲノムを数学的なグラフ構造で表現するグラフゲノムをデータ構造として用いることで、ゲノム間の様々な差異をより自然に表現することが可能 …

    Journal of the Visualization Society of Japan 40(156), 14-18, 2020

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  • Spread of antimicrobial resistant <i>Salmonella</i> from poultry to humans in Thailand

    Kongsanan Paweena , Angkititrakul Sunpetch , Kiddee Anong , Tribuddharat Chanwit

    gene positive). …

    Japanese Journal of Infectious Diseases, 2020

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  • Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> blood culture isolates from three hospitals in Turkey

    Gozalan Aysegul , Unaldı Ozlem , Guldemir Dilek , Aydogan Sibel , Kuzucu Cigdem , Cakirlar Fatma Koksal , Açıkgoz Ziya Cibali , Durmaz Riza

    gene was identified in two<i> …

    Japanese Journal of Infectious Diseases, 2020

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  • Prevalence of Cereulide-Producing <i>Bacillus cereus</i> in Pasteurized Milk  [in Japanese]

    Shimojima Yukako , Hirai Akihiko , Suzuki Jun , Sadamasu Kenji , Kodo Yukihiro , Soeda Kana , Koike Hiroshi , Kanda Maki , Hayashi Hiroshi , Nishino Yukari , Fukui Rie , Kuroda Sumiyo

    <p>パスチャライズド牛乳による<i>Bacillus cereus</i>食中毒のリスクを把握する目的で,汚染実態および分離菌株のセレウリド産生性を調査した.国産パスチャライズド牛乳14製品101検体中66検体(65.3%)から<i>B. cereus</i>が分離された.分離された<i>B. cereus</i> …

    Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 61(5), 178-182, 2020

    J-STAGE  Ichushi Web 

  • Identification of nontuberculous mycobacteria using <i>secA1</i>  [in Japanese]

    TODA Keizo , NOMURA Haruka , SOMETANI Kayoko

    <p>分子生物学的手法の普及により<i>Mycobacterium</i>は5属に再編成され,菌種も188種以上に増加した。そのため,従来の遺伝子検査やフェノタイプ検査では,非結核性抗酸菌の同定が困難になっている。そこで,ハウスキーピング遺伝子の一つであるsecA1遺伝子を用いた,系統樹解析やBLAST解析で同定を試みた。その結果,DDH法や16S rRNA遺伝 …

    Japanese Journal of Medical Technology 69(4), 516-526, 2020

    J-STAGE  Ichushi Web 

  • Serum Bilirubin in the Czech Population ― Relationship to the Risk of Myocardial Infarction in Males ―

    Eremiasova Lenka , Hubacek Jaroslav A , Danzig Vilém , Adamkova Věra , Mrazova Lenka , Pitha Jan , Lanska Věra , Cífková Renata , Vitek Libor

    … b><i>Background:</i></b>The potential antiatherogenic role of bilirubin is generally acknowledged, so the aim of this study was to determine serum bilirubin concentrations and the prevalence of Gilbert syndrome (GS) in the Czech general population with particular reference to its relationship to the risk of myocardial infarction (MI).</p><p><b><i>Methods and Results:</i></b>Biochemical markers were analyzed in 2 independent Czech post-MONICA studies (in total, n=3,311), and in 741 male MI …

    Circulation Journal 84(10), 1779-1785, 2020

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  • A primer set for quantification of environmental DNA of a marine mammal, Dugong dugon  [in Japanese]

    Hiraishi Yumiko , Ozawa Hiroyuki , Wakai Yoshihito , Yamanaka Hiroki , Maruyama Atsushi

    <p>沖縄周辺での地域絶滅が危惧される中型海棲哺乳類ジュゴン(Dugong dugon)の環境 DNA分析による分布調査を可能にすべく、ジュゴン由来の DNAを特異的に検出する PCRプライマーセットの開発を試みた。データベース上の DNA配列情報をもとにジュゴンに特異的な配列にプライマーセットを設計し(チトクロム b領域、増幅産物長: 138 bp)、プライマーセットの有効性と特異性 …

    Japanese Journal of Conservation Ecology 25(1), n/a, 2020

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  • A Multiplex RT-Nested PCR Assay with Newly Designed Primers to Detect and Analyze Human Parainfluenza Viruses Type 1, 2, 3, and 4 from Clinical Specimens  [in Japanese]

    KABURAGI Yasuo , UENO Hiroyuki , KAETSU Akihiko , TOMARI Kentaro , KIKUCHI Koji , KOBORI Sumie , MIYAZAKI Motonobu

    <p> ヒトパラインフルエンザウイルス(Human Parainfluenza Virus:HPIV)は4 つの型に分類されており,遺伝子検査には,文献で報告された型別可能なMultiplex-RT-Nested-PCR 法(既報方法)が広く用いられている.しかしながら既報方法で得られる増幅産物の塩基配列は短く,特に3 型は解析に供する塩基配列が 60 塩基であることから系統樹解析の実 …

    Kansenshogaku Zasshi 94(1), 86-96, 2020

    J-STAGE  Ichushi Web 

  • A case of Hemoglobinopathy HbE-Saskatoon [β 22 Glu→Lys] With a Falsely Low HbA1c Value  [in Japanese]

    Izutsu Takuma , Ito Hiroyuki , Kusano Eiji , Matsumoto Suzuko , Antoku Shinichi , Mori Toshiko

    <p>症例は68歳男性,左下肢の発赤・腫脹を認め外来を受診した.蜂窩織炎の診断で抗生剤投与を行い症状は改善したが,その後も同部位の蜂窩織炎を再発し,入院加療を要した.入院時の随時血糖値が341 mg/dLと高値であったが,イオン交換高速液体クロマトグラフィ(High performance liquid chromatography,以下HPLC)法による自動ヘモグロビン分析でHbA1 …

    Journal of the Japan Diabetes Society 63(7), 445-450, 2020

    J-STAGE  Ichushi Web 

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