Search Results 1-20 of 679

  • The Indian cobra reference genome and transcriptome enables comprehensive identification of venom toxins

    Kushal Suryamohan , Sajesh P. Krishnankutty , Joseph Guillory , Matthew Jevit , Markus S. Schröder , Meng Wu , Boney Kuriakose , Oommen K. Mathew , Rajadurai C. Perumal , Ivan Koludarov , Leonard D. Goldstein , Kate Senger , Mandumpala Davis Dixon , Dinesh Velayutham , Derek Vargas , Subhra Chaudhuri , Megha Muraleedharan , Ridhi Goel , Ying-Jiun J. Chen , Aakrosh Ratan , Peter Liu , Brendan Faherty , Guillermo de la Rosa , Hiroki Shibata , Miriam Baca , Meredith Sagolla , James Ziai , Gus A. Wright , Domagoj Vucic , Sangeetha Mohan , Aju Antony , Jeremy Stinson , Donald S. Kirkpatrick , Rami N. Hannoush , Steffen Durinck , Zora Modrusan , Eric W. Stawiski , Kristen Wiley , Terje Raudsepp , R. Manjunatha Kini , Arun Zachariah , Somasekar Seshagiri

    … We report a de novo near-chromosomal genome assembly of Naja naja, the Indian cobra, a highly venomous, medically important snake. … Of the 23,248 predicted protein-coding genes, 12,346 venom-gland-expressed genes constitute the 'venom-ome' and this included 139 genes from 33 toxin families. …

    Nature Genetics 52(1), 106-117, 2020-01-06

    IR 

  • Alternative mRNA Splicing in Three Venom Families Underlying a Possible Production of Divergent Venom Proteins of the Habu Snake, Protobothrops flavoviridis

    Tomohisa Ogawa , Naoko Oda-Ueda , Kanako Hisata , Hitomi Nakamura , Takahito Chijiwa , Shousaku Hattori , Akiko Isomoto , Haruki Yugeta , Shinichi Yamasaki , Yasuyuki Fukumaki , Motonori Ohno , Noriyuki Satoh , Hiroki Shibata

    Snake venoms are complex mixtures of toxic proteins encoded by various gene families that function synergistically to incapacitate prey. … A huge repertoire of snake venom genes and proteins have been reported, and alternative splicing is suggested to be involved in the production of divergent gene transcripts. … In this study, the comprehensive analysis of transcriptomes in the venom gland was achieved by using PacBio sequencing. …

    Toxins 11(10), 581, 2019-10-09

    IR 

  • Many Options, Few Solutions: Over 60 My Snakes Converged on a Few Optimal Venom Formulations

    Agneesh Barua , Alexander S Mikheyev

    Snake venoms are proteinaceous cocktails where the expression of each toxin can be quantified and mapped to a distinct genomic locus and traced for millions of years. … Using a phylogenetic generalized linear mixed model, we analyzed expression data of toxin genes from 52 snake species spanning the 3 venomous snake families and estimated phylogenetic covariance, which acts as a measure of evolutionary constraint. …

    Molecular Biology and Evolution 36(9), 1964-1974, 2019-05-20

    IR 

  • Snake venom NAD glycohydrolases: primary structures, genomic location, and gene structure

    Ivan Koludarov , Steven D. Aird

    … NAD glycohydrolase (EC 3.2.2.5) (NADase) sequences have been identified in 10 elapid and crotalid venom gland transcriptomes, eight of which are complete. … As in Aplysia kurodai ADP-ribosyl cyclase and vertebrate CD38 genes, snake venom NADase genes comprise eight exons; … While many vertebrate CD38s are multifunctional, liberating both ADP-ribose and small quantities of cyclic ADP-ribose (cADPR), snake venom CD38 homologs are dedicated NADases. …

    PeerJ (7), e6154, 2019-02-06

    IR 

  • Organic and Peptidyl Constituents of Snake Venoms: The Picture Is Vastly More Complex Than We Imagined

    Alejandro Villar-Briones , Steven Aird

    … Small metabolites and peptides in 17 snake venoms (Elapidae, Viperinae, and Crotalinae), were quantified using liquid chromatography-mass spectrometry. … Each venom contains >900 metabolites and peptides. … Many small organic compounds are present at levels that are probably significant in prey envenomation, given that their known pharmacologies are consistent with snake envenomation strategies. …

    Toxins 10(10), 392, 2018-09-26

    IR 

  • Nuclease activity in snake venoms  [in Japanese]

    財津 佳史 , 上田 翔大 , 倉岡 功 , 塩井(青木) 成留実 , Zaitsu Yoshifumi , Ueda Shota , Kuraoka Isao , Shioi Narumi

    福岡大学理学集報 = Fukuoka University science reports 48(2), 59-68, 2018-09

    IR 

  • The habu genome reveals accelerated evolution of venom protein genes

    Hiroki Shibata , Takahito Chijiwa , Naoko Oda-Ueda , Hitomi Nakamura , Kazuaki Yamaguchi , Shousaku Hattori , Kazumi Matsubara , Yoichi Matsuda , Akifumi Yamashita , Akiko Isomoto , Kazuki Mori , Kosuke Tashiro , Satoru Kuhara , Shinichi Yamasaki , Manabu Fujie , Hiroki Goto , Ryo Koyanagi , Takeshi Takeuchi , Yasuyuki Fukumaki , Motonori Ohno , Eiichi Shoguchi , Kanako Hisata , Noriyuki Satoh , Tomohisa Ogawa

    … Several snake lineages developed elaborate venom systems to deliver complex protein mixtures for prey capture. … To understand mechanisms involved in snake venom evolution, we decoded here the ~1.4-Gb genome of a habu, Protobothrops flavoviridis. … We identified 60 snake venom protein genes (SV) and 224 non-venom paralogs (NV), belonging to 18 gene families. …

    Scientific Reports 8(1), 11300, 2018-07-26

    IR 

  • Optimisation of antithrombin resistance assay as a practical clinical laboratory test : Development of prothrombin activator using factors Xa/Va and automation of assay

    Tamura S. , Suga Y. , Tanamura M. , Murata-Kawakami M. , Takagi Y. , Hottori Y. , Kakihara M. , Suzuki S. , Takagi A. , Kojima T.

    ファイル公開:2019-06-01

    International Journal of Laboratory Hematology 40(3), 312-319, 2018-06

    IR 

  • Inhibition of crude viper venom action by silver nanoparticles: A biophysical and biochemical study

    Hingane Vrushali C. , Pangam Dhanashri , Dongre Prabhakar M.

    … <p>This investigation understands the interaction between lyophilized crude Viper snake venom (<i>Doboia russellie</i>) and Silver nanoparticles (SNPs) using biophysical and biochemical approaches. … Interaction of SNPs and viper venom was resulted in the formation of complex which was confirmed by using DLS technique. …

    Biophysics and Physicobiology 15(0), 204-213, 2018

    J-STAGE 

  • Clinical Condition and Management of 114 Mamushi (<i>Gloydius blomhoffii</i>) Bites in a General Hospital in Japan

    Chiba Takahito , Koga Hidenobu , Kimura Nanae , Murata Maho , Jinnai Shunichi , Suenaga Asako , Kohda Futoshi , Furue Masutaka

    … <p><b>Objective </b>Mamushi (<i>Gloydius blomhoffii</i>) snakebite is the most common type of snake injury in Japan and is also seen in China and Korea. … Although the components of Mamushi venom have been investigated, epidemiological and clinical descriptions still remain limited in the English literature. …

    Internal Medicine 57(8), 1075-1080, 2018

    J-STAGE  Ichushi Web 

  • Development of a Simple Permeability Assay Method for Snake Venom-induced Vascular Damage

    SATO Kae , KODAMA Ayuki , KASE Chikako , HIRAKAWA Satoshi , ATO Manabu

    … We have developed a novel bioassay method for the detection of snake venom based on the permeability of endothelial cell monolayers cultured in Transwell cell culture inserts. …

    Analytical Sciences 34(3), 323-327, 2018

    J-STAGE  Ichushi Web 

  • Broad-spectrum antiviral agents: secreted phospholipase A(2) targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane

    Chen Ming , Aoki-Utsubo Chie , Kameoka Masanori , Deng Lin , Terada Yutaka , Kamitani Wataru , Sato Kei , Koyanagi Yoshio , Hijikata Makoto , Shindo Keiko , Noda Takeshi , Kohara Michinori , Hotta Hak

    … Here we show that the CM-II isoform of secreted PLA(2) obtained from Naja mossambica mossambica snake venom (CM-II-sPLA(2)) possesses potent virucidal (neutralising) activity against HCV, DENV and JEV, with 50% inhibitory concentrations (IC50) of 0.036, 0.31 and 1.34 ng/ ml, respectively. …

    Scientific Reports (7), 15931, 2017-11-21

    IR 

  • Population Genomic Analysis of a Pitviper Reveals Microevolutionary Forces Underlying Venom Chemistry

    Steven D. Aird , Jigyasa Arora , Agneesh Barua , Lijun Qiu , Kouki Terada , Alexander S. Mikheyev

    … Many venom gene families, however, have undergone duplication, and are often deployed in doses vastly exceeding the LD50 for most prey species, which should reduce the strength of positive selection. … Here, we contrast these selective regimes using snake venoms, which consist of rapidly evolving protein formulations. …

    Genome Biology and Evolution 9(10), 2640-2649, 2017-09-27

    IR 

  • Comprehensive Proteomic Analysis of Venoms from Seven Snakes Indigenous to Southeast Asia.  [in Japanese]

    Cannizzaro L. , Pisitkun T. , Devabhakuri A. , Sopitthummakhun K. , Chanhome L. , Pakmanee N. , Chaiyabutr N. , Elias J.E. , Payne D.M. , Sitprija V.

    Abstracts for Annual Meeting of Japanese Proteomics Society 2017(0), 217-217, 2017

    J-STAGE 

  • Lethal, Hemorrhagic, and Necrotic Effects of <i>Montivipera bornmuelleri</i> Venom

    ABI-RIZK Alain , RIMA Mohamad , HRAOUI BLOQUET Souad , SADEK Riyad , SLEIMAN Yvonne , FAJLOUN Ziad , HLEIHEL Walid

    venom has been determined by performing intravenous, intraperitoneal (IP), and subcutaneous injections. … venom. … venom is similar to that of <i>Daboia palaestinae</i> … venom (IP LD<sub>50</sub>=1.9 mg/kg) and is higher than that of <i>Macrovipera lebetina</i> … venom (IP LD<sub>50</sub>=7.58 mg/kg) and that the hemorrhagic activity of <i>M. …

    Current Herpetology 36(1), 58-62, 2017

    J-STAGE 

  • Analytical Applications of Microfluidic Vascular Models  [in Japanese]

    SASAKI Naoki , SATO Kae

    マイクロ流路を用いて血管モデルを構築し,分析化学的応用を図ってきた.電池及び制御系が組み込まれた小型ポンプを用い,手のひらサイズの細胞培養システムを開発した.これを用いて血管内皮細胞を流路内で培養し,シリンジポンプを用いた系と同等の細胞増殖速度及びタンパク質発現を実現した.さらに,血管に加えてリンパ管の要素を有するモデルを作製し,血管及びリンパ管のバリア機能の評価に利用できることを示した.これをハ …

    BUNSEKI KAGAKU 65(5), 241-247, 2016

    J-STAGE  Ichushi Web 

  • An account of Hideyo Noguchi's brief return to Japan in 1915, based on THE SHIKWA GAKUHO  [in Japanese]

    加來 洋子 , 山口 秀紀 , 石橋 肇 , 卯田 昭夫 , 鈴木 正敏 , 下坂 典立 , 田中 晃伸 , 渋谷 鑛 , Yohko Kaku , Hidenori Yamaguchi , Hajime Ishibashi , Akio Uda , Masatoshi Suzuki , Michiharu Shimosaka , Akinobu Tanaka , Koh Shibutani , 日本大学松戸歯学部 , 日本大学松戸歯学部 , 日本大学松戸歯学部 , 日本大学松戸歯学部 , 日本大学松戸歯学部 , 日本大学松戸歯学部 , 日本大学松戸歯学部 , 日本大学松戸歯学部 , Nihon University School of dentistry at Matsudo , Nihon University School of dentistry at Matsudo , Nihon University School of dentistry at Matsudo , Nihon University School of dentistry at Matsudo , Nihon University School of dentistry at Matsudo , Nihon University School of dentistry at Matsudo , Nihon University School of dentistry at Matsudo , Nihon University School of dentistry at Matsudo

    野口英世(1876〜1928)は,明治29年(1896)に20歳で上京し,医術開業試験(前期),翌30年(1897)に後期試験に合格した.その後,高山歯科医学院で血脇守之助に講師(病理学と薬理学)として採用される.明治32年には横浜の海港検疫所の臨時検疫医官補として勤務し,中国にも赴き帰国後,海外留学を目指していた.アメリカ留学がかなうのは明治33年(1900)のことである.渡米後,蛇毒の研究,免 …

    日本歯科医史学会会誌 31(2), 123-132, 2015-09-15

    IR  Ichushi Web 

  • Flavorase, a novel non-haemorrhagic metalloproteinase in Protobothrops flavoviridis venom, is a target molecule of small serum protein-3

    Shioi Narumi , Nishijima Ayumi , Terada Shigeyuki

    The journal of biochemistry 158(1), 37-48, 2015-07

    Ichushi Web 

  • Biochemical and functional characterization of Bothropoidin : the first haemorrhagic metalloproteinase from Bothrops pauloensis snake venom

    Gomes Mario Sergio R. , Naves de Souza Dayane L. , Guimaraes Denise O. [他]

    The journal of biochemistry 157(3), 137-149, 2015-03

    Ichushi Web 

  • Western Blot Analysis of HR1b in the Venom of Snake Okinawa Habu  [in Japanese]

    大城 聡子 , 上江洲 由美子 , 盛根 信也 [他]

    沖縄県衛生環境研究所報 (48), 42-45, 2014

    Ichushi Web 

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