Search Results 1-20 of 266

  • Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein.

    SEITA Yasunari , MORIMURA Toshifumi , WATANABE Naoki , IWATANI Chizuru , TSUCHIYA Hideaki , NAKAMURA Shinichiro , SUZUKI Toshiharu , YANAGISAWA Daijiro , TSUKIYAMA Tomoyuki , NAKAYA Masataka , OKAMURA Eiichi , MUTO Masanaga , EMA Masatsugu , NISHIMURA Masaki , TOOYAMA Ikuo

    Alzheimer's disease (AD) is the most common cause of dementia and understanding its pathogenesis should lead to improved therapeutic and diagnostic methods. Although several groups have developed tran …

    Journal of Alzheimer's disease : JAD 75(1), 45-60, 2020-06

    IR 

  • Evaluation of frozen-thawed mouse sperm using CPA solution containing antifreeze protein  [in Japanese]

    安齋 政幸 , 山鹿 優真 , 掛井 友輝央 , 堀 光一郎 , 山崎 脩杜 , 津田 栄 , 松本 和也

    [要旨]本研究では、マウス凍結精子保存溶液に添加されるSkimMilkの代替保護物質として期待される不凍タンパク質(AFP)が凍結融解後の精子細胞膜に及ぼす影響を形態学的観察により評価した。その結果、AFPIIIが添加された場合において精子細胞膜の著しい損傷がないことを示した。さらに体外受精により40%以上の受精が可能であり正常に発生した。これらの結果から、マウス精子保存液へのAFPIII添加が細 …

    近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kindai University (25), 17-25, 2020-03-31

    IR 

  • Evaluation of frozen-thawed mouse sperm using CPA solution containing antifreeze protein  [in Japanese]

    安齋 政幸 , 山鹿 優真 , 掛井 友輝央 , 堀 光一郎 , 山﨑 脩杜 , 津田 栄 , 松本 和也

    Memoirs of Institute of Advanced Technology, Kinki University = 近畿大学先端技術総合研究所紀要 (25), 17-25, 2020-03

  • The importance of sperm cryopreservation for fertility preservation  [in Japanese]

    柴崎 世菜 , 佐藤 愛 , 中村 祐介 , 服部 裕充 , 中條 友紀子 , 片岡 万里乃 , 奥山 紀之 , 青野 展也 , 田井 俊宏 , 橋本 朋子 , 戸屋 真由美 , 五十嵐 秀樹 , 京野 廣一

    日本がん・生殖医療学会誌 = Journal of fertility preservation 3(1), 31-35, 2020

  • Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm

    Umehara Takashi , Tsujita Natsumi , Shimada Masayuki

    … In most mammals, the male to female sex ratio of offspring is about 50% because half of the sperm contain either the Y chromosome or X chromosome. … Although overall gene expression is lower in sperm than in somatic cells, transcription is activated selectively in round spermatids. … By regulating the expression of specific genes, we hypothesized that the X chromosome might exert functional differences in sperm that are usually masked during fertilization. …

    PLoS Biology 17(8), e3000398, 2019-08-13

    IR 

  • Recovery of Spermatogenesis Following Cancer Treatment with Cytotoxic Chemotherapy and Radiotherapy

    Okada Keisuke , Fujisawa Masato

    Sperm cryopreservation is an effective method for preserving spermatozoa. …

    World Journal of Mens Health 37(2), 166-174, 2019-05

    IR 

  • Gene Expression and Protein Synthesis in Mitochondria Enhance the Duration of High-Speed Linear Motility in Boar Sperm

    Zhu Zhendong , Umehara Takashi , Okazaki Tetsuji , Goto Masaaki , Fujita Yoko , Hoque S. A. Masudul , Kawai Tomoko , Zeng Wenxian , Shimada Masayuki

    Sperm motility patterns are continuously changed after ejaculation to fertilization in the female tract. … Hyperactivated motility is induced with high glucose medium in vitro or the oviduct fluids in vivo, whereas sperm maintain linear motility in the seminal plasma or the uterine fluids containing low glucose. …

    Frontiers in Psychology (10), 252, 2019-03-12

    IR 

  • Preclinical evaluation of a new cryopreservation container for a limited number of human spermatozoa

    NAKATA Kumiko , YOSHIDA Kaoru , YAMASHITA Naoki

    … The aim of this study was to develop a new container for cryopreservation of a limited number of spermatozoa. … To evaluate the efficacy and safety of this new container, we performed preclinical evaluations using human sperm or mouse oocytes and sperm. … First, using human sperm that was frozen and then thawed, we demonstrated that the sperm recovery rate using the new container was 96.7% (58/60), which was significantly higher (P < …

    Journal of Reproduction and Development 65(4), 297-304, 2019

    J-STAGE 

  • Clinical outcome and analysis of sperm cryopreservation for male cancer patients  [in Japanese]

    DAHAL(SANADA) Sachiko , NAKAYAMA Takahiro , KOHAMA Nami , HAMADA Hironori , INOUE Takuya , YAMADE Ichiro , YANO Juri , HATAYAMA Hiroshi

    <p>医療の進歩によりがんサバイバーのQOLが重視され,がんサバイバーの妊孕性温存は重要な課題である.当院では,2001年よりがん患者を対象とした精子凍結保存を行ってきた.今回,過去17年間における妊孕性温存のための精子凍結保存の実態調査,それを用いた不妊治療の成績について検討した.【方法・対象】当院で2001~2018年2月までの間に妊孕性温存のため精子凍結を行ったがん患者369例の …

    ADVANCES IN OBSTETRICS AND GYNECOLOGY 71(1), 1-8, 2019

    J-STAGE  Ichushi Web 

  • SPERM CRYOPRESERVATION FOR A GENDER IDENTITY DISORDER PATIENT PRIOR TO SEX REASSIGNMENT SURGERY : A CASE REPORT  [in Japanese]

    大坂 晃由 , 小堀 善友 , 新井 学 , 岡田 弘

    … We describe the first case of a 25-year-old patient with gender identity disorder (GID) who underwent cryopreservation of sperm prior to male-to-female (MTF) sex reassignment surgery in Japan. … The patient wanted to freeze sperm to keep open the option of conceiving a child in the future. … The ethics committee of our institution discussed the case and officially approved cryopreservation of sperm before sex reassignment surgery. …

    泌尿器科紀要 = Acta urologica Japonica 64(11), 451-453, 2018-11

    IR  DOI  Ichushi Web 

  • Effect of Various Coconut (Cocos nucifera) Milk Concentrations Instead of Egg Yolk on Freezability and Fertility of Frozen Holstein Bull Spermatozoa

    EL–SHAMAA Ibrahim , IBRAHIM Mostafa , EL–SHARAWY Mohamed , MAHMOUD Raed , KUBOTA Kaiyu , YAMAUCHI Nobuhiku , El–SIEFY El–Shenawy

    … Semen from five Holstein bulls was collected twice weekly and ejaculates with 75% progressive motility and more 85% normal sperm morphology prior to cryopreservation were pooled in order to have sufficient semen for a replicate and to eliminate the bull effect. … Semen was diluted to 80 million sperm/ml packaged into 0.25ml straws, cooled held at 5ºC for 4 h, and then frozen in liquid nitrogen and stored at –196ºC until for artificial insemination. …

    九州大学大学院農学研究院紀要 63(2), 265-270, 2018-09-01

    IR 

  • Protective Effect of Trehalose on Canine Spermatozoa in Cryopreservation

    PARK Kang–Sun , CHUN Ju Lan , KIM Eun Young , LEE Ji Hye , LEE Hyung Suk , YAMAUCHI Nobuhiko , LEE Kyung–Bon , KIM Min Kyu

    Cryopreservation of canine spermatozoa allows to preserve male genetic materials and contributes to conserve genetic materials of superior males or extinct animals in breeding management by using the frozen spermatozoa at time needed. … However, spermatozoa may undergo multiple steps of possible damage during cryopreservation such as ice crystal formation. …

    九州大学大学院農学研究院紀要 63(1), 53-60, 2018-02-27

    IR 

  • Freezing sperm in short straws reduces storage space and allows transport in dry ice

    KANEKO Ryosuke , KAKINUMA Toshie , SATO Sachiko , JINNO-OUE Atsushi

    … Efficient cryopreservation and transportation of mouse sperm are among the most desirable strategies for current and future research on mouse genetics. … However, the current method for sperm cryopreservation uses an 11-cm plastic straw, which is a bulky and fragile container. …

    Journal of Reproduction and Development 64(6), 541-545, 2018

    J-STAGE  Ichushi Web 

  • Sperm cryopreservation in pediatric and young adult patients in preparation for allogeneic hematopoietic stem cell transplantation  [in Japanese]

    Umeda Katsutsugu , Adachi Souichi , Goi Rie , Tanaka Kuniaki , Kozuki Kagehiro , Kubota Hirohito , Hamabata Takayuki , Daifu Tomoo , Kato Itaru , Hiramatsu Hidefumi

    <p>精子保存が開始された2008年から2015年の間に当科で造血幹細胞移植を計画した移植時10歳以上の男性患者19例に対する精子保存の実施状況について後方視的に検討した.19例の年齢は10–27歳(中央値15歳)で,原疾患は血液悪性疾患8例,非悪性疾患11例であった.19例中10例が精子保存の説明を受け,うち7例が保存を試み,5例に実施された.骨髄破壊的前処置群10例中6例,骨髄非破 …

    The Japanese Journal of Pediatric Hematology / Oncology 55(3), 279-283, 2018

    J-STAGE  Ichushi Web 

  • Fertility preservation by testicular sperm extraction in a young patient with extraosseous Ewing's sarcoma of the pelvis  [in Japanese]

    Tomida Akimasa , Shirai Toshiharu , Kubo Toshikazu , Ichioka Kentarou , Hosoi Hajime , Miyachi Mitsuru , Yagyu Shigeki , Tsuchiya Kunihiko , Iehara Tomoko , Ohara Masato , Ishibashi Hidenobu , Tonomura Jin , Terauchi Ryu

    <p>小児がん治療による妊孕性喪失の危険性が高い男児の場合,妊孕性温存のため治療開始前に精子凍結保存が望ましい.我々は,精巣内精子採取術 (TESE) により精子凍結保存を施行した骨外性ユーイング肉腫ファミリー腫瘍の症例を経験した.症例は17歳男性.臀部痛と膀胱直腸障害で発症し,仙骨部腫瘍の生検によりユーイング肉腫と診断した.妊孕性温存療法として化学療法開始前にマスターベーションによる …

    The Japanese Journal of Pediatric Hematology / Oncology 55(3), 320-323, 2018

    J-STAGE  Ichushi Web 

  • Expression and localization of aquaporins 3 and 7 in bull spermatozoa and their relevance to sperm motility after cryopreservation  [in Japanese]

    FUJII Takashi , HAYAKAWA Hiroyuki , TAKAHASHI Kenichi , TAKAHASHI Yoshiyuki , SAWAI Ken , HIRAYAMA Hiroki , FUKUDA Shigeo , KAGEYAMA Soichi , NAITO Akira , YOSHINO Hitomi , MORIYASU Satoru , YAMAZAKI Takashi , SAKAMOTO Kozo

    <p>【目的】牛の人工授精では,凍結保存精液が利用されているが,現在の凍結保存技術でも凍結融解後のウシ精子は約30–50%が死滅する。ウシ精子凍結保存技術をさらに高度化・効率化するためには,ウシ精子の凍結傷害に関わるメカニズムについて,分子レベルでの理解が必要である。本研究では,水や耐凍剤であるグリセリン(GL)を効率的に透過させる膜内在性タンパク質のアクアポリン(AQP)3および7に …

    The Journal of Reproduction and Development Supplement 111(0), P-17-P-17, 2018

    J-STAGE 

  • Expression and localization of aquaporins 3 and 7 in bull spermatozoa and their relevance to sperm motility after cryopreservation

    FUJII Takashi , HAYAKAWA Hiroyuki , TAKAHASHI Kenichi , TAKAHASHI Yoshiyuki , SAWAI Ken , HIRAYAMA Hiroki , FUKUDA Shigeo , KAGEYAMA Soichi , NAITO Akira , YOSHINO Hitomi , MORIYASU Satoru , YAMAZAKI Takashi , SAKAMOTO Kozo

    … However, despite current technical advances, cryopreservation continues to damage bull spermatozoa, resulting in a loss of approximately 30 to 50% of viable spermatozoa post thawing. … To further improve the efficiency of cryopreservation of bull spermatozoa, understanding the molecular mechanisms underlying the cryobiological properties that affect cryoinjuries during cryopreservation process of bull spermatozoa is required. …

    Journal of Reproduction and Development 64(4), 327-335, 2018

    J-STAGE  Ichushi Web 

  • Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa

    ÁLVAREZ-RODRIGUEZ Manuel , VICENTE-CARRILLO Alejandro , RODRIGUEZ-MARTINEZ Heriberto

    … Hyaluronan (hyaluronic acid, HA) apparently improves sperm survival <i>in vitro</i> … (oviduct), maintaining sperm motility and inducing capacitation, but not acrosome exocytosis, either by direct action as a macromolecule or via CD44 membrane receptors. … and (iii) whether the addition of HA, of molecular size comparable to that produced in the oviduct sperm reservoir (0.0625 to 2.0 mg/ml; …

    Journal of Reproduction and Development 64(4), 351-360, 2018

    J-STAGE  Ichushi Web 

  • Identification of quantitative trait loci associated with the susceptibility of mouse spermatozoa to cryopreservation

    LIU Jinsha , MOCHIDA Keiji , HASEGAWA Ayumi , INOUE Kimiko , OGURA Atsuo

    … These regions included at least 15 genes involved with testicular expression and possibly with capacitation or sperm motility. … This result is consistent with the fact that C57BL/6J frozen-thawed spermatozoa recover their fertilization capacity following treatment with methyl-β-cyclodextrin to enhance sperm capacitation. …

    Journal of Reproduction and Development 64(2), 117-127, 2018

    J-STAGE  Ichushi Web 

  • Characterization of the functions and proteomes associated with membrane rafts in chicken sperm

    Ushiyama Ai , Tajima Atsushi , Ishikawa Naoto , Asano Atsushi , 田島 淳史 , 石川 尚人 , 浅野 敦之

    … Despite the central role of membrane functions in multiple cellular processes in sperm, their molecular mechanisms are poorly understood. … Studies of the functional characterization of membrane rafts in mammalian sperm have demonstrated roles in sperm-egg binding and the acrosomal reaction. … Recently, our biochemical and cell biological studies showed that membrane rafts are present and might play functional roles in chicken sperm. …

    PLOS ONE 12(11), e0186482, 2017-11

    IR 

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