Search Results 1-20 of 1464

  • Therapeutic benefit of Muse cells in a mouse model of amyotrophic lateral sclerosis

    Yamashita Toru , Kushida Yoshihiro , Wakao Shohei , Tadokoro Koh , Nomura Emi , Omote Yoshio , Takemoto Mami , Hishikawa Nozomi , Ohta Yasuyuki , Dezawa Mari , Abe Koji

    … In G93A-transgenic ALS mice, intravenous injection of 5.0x10(4) cells revealed successful homing of human-Muse cells to the lumbar spinal cords, mainly at the pia-mater and underneath white matter, and exhibited glia-like morphology and GFAP expression. …

    Scientific Reports 10(1), 17102, 2020-10-13

    IR 

  • CCN3 (NOV) Drives Degradative Changes in Aging Articular Cartilage

    Kuwahara Miho , Kadoya Koichi , Kondo Sei , Fu Shanqi , Miyake Yoshiko , Ogo Ayako , Ono Mitsuaki , Furumatsu Takayuki , Nakata Eiji , Sasaki Takako , Minagi Shogo , Takigawa Masaharu , Kubota Satoshi , Hattori Takako

    … During aging, the expression of Ccn3 mRNA in mouse primary chondrocytes from knee cartilage increased and showed a positive correlation with p21 and p53 mRNA. … We have produced cartilage-specific CCN3-overexpressing transgenic mice, and found degradative changes in knee joints within two months. … Inflammatory gene expression was found even in the rib chondrocytes of three-month-old transgenic mice. …

    International Journal of Molecular Sciences 21(20), 7556, 2020-10-13

    IR 

  • Transcranial Cortex-Wide Fluorescence Imaging through a Fully Intact Skull, as a Powerful Tool for Functional Mapping: Less Invasive Macroscopic Imaging of Cortical Ca2+ Dynamics

    MONAI Hiromu

    … In our laboratory, we use a transgenic mouse line (G7NG817) that expresses G-CaMP7, a Ca2+ sensor protein, in astrocytes and a portion of the excitatory neurons, to visualize cortical function in vivo. … Using the G7NG817 mouse, we have reported that specific neuronal populations in the cortex were activated by distinct modalities, such as light, sound, or whisker mechanical stimulation. …

    お茶の水女子大学自然科学報告 71(特別号), 39-50, 2020-09

    IR 

  • The integrity of cochlear hair cells is established and maintained through the localization of Dia1 at apical junctional complexes and stereocilia

    Ninoyu Yuzuru , Sakaguchi Hirofumi , Lin Chen , Suzuki Toshiaki , Hirano Shigeru , Hisa Yasuo , Saito Naoaki , Ueyama Takehiko

    … In the present study, we demonstrated the localization of endogenous Dia1 and the constitutively active DIA1 mutant in the cochlea, using transgenic mice expressing FLAG-tagged DIA1(R1204X) (DIA1-TG). … Furthermore, we established a knock-in (KI) mouse line expressing AcGFP-tagged DIA1(R1213X) (DIA1-KI) and confirmed mutant localization at AJCs and the tips of stereocilia in HCs. …

    Cell Death & Disease 11(7), 536, 2020-07-16

    IR 

  • Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein.

    SEITA Yasunari , MORIMURA Toshifumi , WATANABE Naoki , IWATANI Chizuru , TSUCHIYA Hideaki , NAKAMURA Shinichiro , SUZUKI Toshiharu , YANAGISAWA Daijiro , TSUKIYAMA Tomoyuki , NAKAYA Masataka , OKAMURA Eiichi , MUTO Masanaga , EMA Masatsugu , NISHIMURA Masaki , TOOYAMA Ikuo , 清田 弥寿成 , 守村 敏史 , 渡邊 直希 , 岩谷 千鶴 , 土屋 英明 , 中村 紳一郎 , 柳沢 大治郎 , 築山 智之 , 中家 雅隆 , 岡村 永一 , 武藤 真長 , 西村 正樹 , 遠山 育夫

    … Although several groups have developed transgenic mouse models overexpressing the human amyloid-β precursor protein (APP) gene with AD mutations, with and without presenilin mutations, as well as APP gene knock-in mouse models, these animals display amyloid pathology but do not show neurofibrillary tangles or neuronal loss. … This presumably is due to differences between the etiology of the aged-related human disease and the mouse models. …

    Journal of Alzheimer's disease : JAD 75(1), 45-60, 2020-06

    IR 

  • Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice

    Noda Taichi , Lu Yonggang , Fujihara Yoshitaka , Oura Seiya , Koyano Takayuki , Kobayashi Sumire , Matzuk Martin M. , Ikawa Masahito

    Transgenic mice which expressed mouse Sof1, Tmem95, and Spaca6 rescued the sterility of Sof1, Tmem95, and Spaca6 KO males, respectively. …

    Proceedings of the National Academy of Sciences of the United States of America 117(21), 11493-11502, 2020-05-26

    IR 

  • The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion

    Nagai Yuma , Takayama Kaito , Nishitani Naoya , Andoh Chihiro , Koda Masashi , Shirakawa Hisashi , Nakagawa Takayuki , Nagayasu Kazuki , Yamanaka Akihiro , Kaneko Shuji

    … Methods: For transduction of 5-HT neurons in wildtype mice, adeno-associated virus (AAV) bearing the mouse tryptophan hydroxylase 2 (TPH2) gene promoter was used. … For transduction in Tph2-tTA transgenic mice, AAVs bearing the tTA-dependent TetO enhancer were used. …

    International Journal of Molecular Sciences 21(6), 2020-03-21

    IR 

  • Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3

    Imayoshi Itaru , Tabuchi Sawako , Matsumoto Mami , Kitano Satsuki , Miyachi Hitoshi , Kageyama Ryoichiro , Yamanaka Akihiro

    … In this report, we show the generation of new mouse strains that express eNpHR3-EYFP fusion proteins after Cre- and/or Flp-mediated recombination to optically inhibit neuronal activity. … In these mouse strains, Cre/Flp recombination induced high levels of opsin expression. … Thus, these eNpHR3 mouse strains offer reliable genetic tools for light-induced inhibiting of neuronal activity in defined sets of neurons. …

    Scientific Reports (10), 2020-02-21

    IR 

  • The matrix vesicle cargo miR-125b accumulates in the bone matrix, inhibiting bone resorption in mice

    Minamizaki Tomoko , Nakao Yuko , Irie Yasumasa , Ahmed Faisal , Itoh Shota , Sarmin Nushrat , Yoshioka Hirotaka , Nobukiyo Asako , Fujimoto Chise , Niida Shumpei , Sotomaru Yusuke , Tanimoto Kotaro , Kozai Katsuyuki , Sugiyama Toshie , Bonnelye Edith , Takei Yuichiro , Yoshiko Yuji

    … We show that the MV cargo miR-125b accumulates in the bone matrix, with increased accumulation in transgenic (Tg) mice overexpressing miR-125b in osteoblasts. … Overexpressing miR-125b in osteoblasts abrogates bone loss in different mouse models. …

    Communications Biology (3), 30, 2020-01-16

    IR 

  • Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus

    松﨑 仁美 , 谷本 啓司 , Hitomi MATSUZAKI , Daichi Kuramochi , Eiichi Okamura , Katsuhiko Hirakawa , Aki Ushiki , Keiji TANIMOTO

    … We previously showed that a 2.9-kb H19 ICR fragment in transgenic mice was differentially methylated only after fertilization, demonstrating that two separable events, gametic and post-fertilization methylation, occur at the H19 ICR. … A lambda DNA-based artificial fragment that contains the 118-bp sequence, in addition to the previously identified cis elements, could fully replace the function of the H19 ICR in the mouse genome. …

    Epigenetics & Chromatin (13), 2, 2020-01

    IR 

  • Pathophysiology of myeloproliferative neoplasms revealed from mouse models with driver mutations.  [in Japanese]

    Shide Kotaro

    … <p>Since the mouse models with the driver mutations of myeloproliferative neoplasms (MPNs) reproduce various pathological conditions found in MPNs patients such as blood cell increase, splenomegaly, and bone marrow fi brosis, these mouse models are extremely useful as tools to elucidate the mechanism of pathogenesis. …

    Cytometry Research 30(2), 15-20, 2020

    J-STAGE 

  • Novel Silk Materials from Unique Silkworm Varieties  [in Japanese]

    TERAMOTO Hidetoshi

    <p>本稿では,特徴あるカイコ系統を利用した新規シルク素材の開発について2つの例を紹介する。従来の品種改良によって生み出されたカイコ系統「セリシンホープ」は,シルクを構成する接着タンパク質セリシンのみからなる薄い繭を作る。このセリシンホープの繭からは,フィルムや含水ゲルといった素材化が可能な高分子セリシンを得ることができる。化粧品素材や細胞培養基材,組織再生材料などへの利用展開が期待で …

    Oleoscience 20(12), 543-548, 2020

    J-STAGE 

  • Yi-Zhi-Fang-Dai Formula Exerts a Protective Effect on the Injury of Tight Junction Scaffold Proteins <i>in Vitro</i> and <i>in Vivo</i> by Mediating Autophagy through Regulation of the RAGE/CaMKKβ/AMPK/mTOR Pathway

    Chan Yuanjin , Chen Wenjing , Chen Yanjie , Lv Zhongkuan , Wan Wenbin , Li Yaming , Zhang Chunyan

    … We examined the impacts of YZFDF on autophagy-related proteins and the histopathology of Aβ in the APP/PS1 double-transgenic male mice. … oligomers-induced alterations of autophagy in murine mouse brain capillary (bEnd.3) cells were blocked by RAGE small interfering RNA (siRNA). …

    Biological and Pharmaceutical Bulletin 43(12), 1847-1858, 2020

    J-STAGE 

  • Effect of PIERCE1 on colorectal cancer

    PARK Bo Min , KIM Hye Jeong , OH Ja Hyun , ROH Jae-il , LEE Han-Woong

    transgenic (TG) mouse model and then administered azoxymethane with dextran sodium sulfate (DSS) to induce colorectal carcinogenesis via promoting mutations in <i>Apc</i> …

    Experimental Animals 69(4), 414-422, 2020

    J-STAGE 

  • Visualization of mechanical stress-mediated Ca<sup>2+</sup> signaling in the gut using intravital imaging

    AIHARA Yoshiko , FUKUDA Yota , TAKIZAWA Akiyoshi , OSAKABE Naomi , AIDA Tomomi , TANAKA Kohichi , YOSHIKAWA Soichiro , KARASUYAMA Hajime , ADACHI Takahiro

    … biosensor yellow cameleon 3.60 (YC3.60)-expressing transgenic mouse model and established a five-dimensional (5D; … The ENS, including the myenteric (Auerbach's) and submucous (Meissner's) plexuses, could be visualized without staining in this mouse line, indicating that the probe produced sufficient fluorescent intensity. …

    Bioscience of Microbiota, Food and Health 39(4), 209-218, 2020

    J-STAGE 

  • Environmental monitoring by using living organisms: Detection of mutagenesis induced by exposure of environmental chemical mixture by using transgenic rodent in vivo assay  [in Japanese]

    AOKI Yasunobu

    … gpt deltaマウスなどのtransgenic rodent in vivo assayは、標的遺伝子上に誘発された突然変異の解析から、各臓器での突然変異頻度や、変異が高頻度に誘発される塩基配列(mutation hotspot)が同定できるなど、複合曝露の影響評価に適した特性をもつ。 …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), S31-4, 2020

    J-STAGE 

  • Reproductive and developmental toxicity in a humanized-transgenic mouse with human placental endocrine function  [in Japanese]

    NAKANISHI Tsuyoshi

    <p>胎盤は妊娠期間中の重要な内分泌組織であるが種差が大きく、ヒトと齧歯類では母体-胎盤-胎仔間のステロイドホルモン動態がかなり異なる。特に胎生期のエストロゲンレベルについては、ヒトでは高値となるが齧歯類ではヒトの1/50~1/100程度と低値である。これはヒトにおいて胎盤が主要なエストロゲン産生(アンドロゲン代謝)臓器として機能するのに対し、齧歯類の胎盤にはエストロゲン合成酵素(アロ …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), S16-4, 2020

    J-STAGE 

  • Involvement of immune tolerance systems in the onset of HLA allele-specific drug-induced immunotoxicity  [in Japanese]

    AOKI Shigeki , KUWAHARA Saki , SUSUKIDA Takeshi , ITO Kousei

    <p>【目的】近年、免疫の関与する特異体質薬物毒性(IDT)の発症リスクとヒト白血球抗原(HLA)多型との関連が多数報告されている。我々はこれまでに、抗HIV薬アバカビルが関与したIDTによる皮疹発症に焦点を当て、キメラ型HLA-B*57:01遺伝子導入マウス(B*57:01-Tg)を用いることで、アバカビルの耳介局所曝露によってIDTが評価可能であることを報告してきた。しかし、アバカ …

    Annual Meeting of the Japanese Society of Toxicology 47.1(0), P-61E, 2020

    J-STAGE 

  • Transgenic mouse model exhibiting weak red fluorescence before and strong green fluorescence after Cre/loxP-mediated recombination

    Akai Ryoko , Saito Michiko , Kohno Kenji , Iwawaki Takao

    … However, a transgenic mouse introduced with the same reporter gene exhibits a weak red fluorescence before and a strong green fluorescence after Cre/loxP-mediated recombination. … This property manifested ubiquitously in this mouse model and was maintained stably in mouse-derived fibroblasts. …

    Experimental Animals 69(3), 306-318, 2020

    J-STAGE  Ichushi Web 

  • Analysis of the transgene insertion pattern in a transgenic mouse strain using long-read sequencing

    Suzuki Osamu , Koura Minako , Uchio-Yamada Kozue , Sasaki Mitsuho

    … <p>Transgene insertion patterns are critical for the analysis of transgenic animals because the influence of transgenes may change depending on the insertion pattern (such as copy numbers and orientations of concatenations) and the insertion position in the genome. … We previously reported a genomic walking strategy to locate transgenes in the genomes of transgenic mice (Exp. …

    Experimental Animals 69(3), 279-286, 2020

    J-STAGE  Ichushi Web 

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