Search Results 1-20 of 105

  • Cell-type-specific targeting strategies for elucidating neural circuits and pathophysiological mechanisms in the marmoset brain  [in Japanese]

    Hara Taiki , Osakada Fumitaka

    <p>我々の脳は,多様な細胞が形態や機能を変化させながら互いに協奏し,複雑な脳機能を発現するダイナミックなネットワークシステムである.このシステムの破綻に起因する多くの中枢神経系疾患は,各国で疾病負荷の原因となり治療法開発が強く望まれている.ダイナミックなネットワークとしてのヒト脳機能解明には,様々な時空間スケールの情報を包括的に解析することで個体レベルの高次脳機能や,他個体との相互作 …

    Folia Pharmacologica Japonica 153(5), 210-218, 2019

    J-STAGE  Ichushi Web 

  • Automating measurements of fluorescent signals in freely moving plant leaf specimens

    Kinoshita Natsuko , Sugita Aki , Lustig Barry , Betsuyaku Shigeyuki , Fujikawa Tetsu , Morishita Tatsuji

    … These techniques, however, are unsuitable for measuring fluorescent signals in time-lapse experiments using plant specimens that move naturally during a course of imaging. … We developed an automated method to measure fluorescent signal intensities in transgenic Arabidopsis plants using a stereomicroscope with standard microscopy software. …

    Plant Biotechnology 36(1), 7-11, 2019

    J-STAGE 

  • Culture and transplantation of spermatogonial stem cells

    Takashima Seiji , Shinohara Takashi

    … Although it became possible to produce transgenic animals using this transplantation technique in 2001, the lack of SSC culture systems prevented efficient genetic manipulation. … Using these techniques, it is now possible to address basic biological questions of SSC biology. …

    Stem Cell Research (29), 46-55, 2018-05

    IR 

  • Inheritance of Bluish Flower Color of Transgenic Chrysanthemum by Interspecific Hybrids

    AIDA Ryutaro , DOUZONO Mitsuru , YOSHIOKA Satoshi , NODA Naonobu , TSUDA Mai , OHSAWA Ryo

    … <p>Bluish transgenic chrysanthemums have already been produced and are expected to be commercialized. … However, little research has been conducted on the crossability of transgenic chrysanthemums or on the heritability of the transgenes involved. … Some transgenic progeny exhibited a modified flower color similar to that of the parent. …

    Japan Agricultural Research Quarterly: JARQ 52(4), 339-345, 2018

    J-STAGE 

  • Contributions of organellar reaction cluster to biomass-based sustainability  [in Japanese]

    Numata Keiji , Tsuchiya Kousuke , Kodama Yutaka

    … <p>Our aim is to establish "Organellar Reaction Cluster" as a novel field of science along with its related foundational techniques. … We will establish techniques for simultaneously modifying the genomes of nuclei, plastids, and mitochondria with transportation of proteins and bioactive molecules into specific organelles. … Using these foundational techniques, the effect of organellar interactions and subcellular localization on metabolism will be quantified. …

    Regulation of Plant Growth & Development 53(1), 80-83, 2018

    J-STAGE 

  • Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: Application to behavioral and developmental studies

    Hara Chiho , Morishita Koudai , Takayanagi-Kiya Seika , Mikami Akihisa , Uchino Keiro , Sakurai Takeshi , Kanzaki Ryohei , Sezutsu Hideki , Iwami Masafumi , Kiya Taketoshi

    … 金沢大学理工研究域自然システム学系Silkmoth, Bombyx mori, is one of the important model insects in which transgenic techniques and the GAL4/UAS system are applicable. … Also, with the refined system, we generated a transgenic strain that expresses tetanus toxin light chain (TeTxLC), which blocks synaptic transmission, under the control of GAL4. …

    Scientific Reports 7(1), 11795, 2017-12-01

    IR  DOI 

  • Electrophysiological analyses of transgenic mice overexpressing KCNJ8 with S422L mutation in cardiomyocytes

    Watanabe Yasuhiro , Matsumoto Akio , Miki Takashi , Seino Susumu , Anzai Naohiko , Nakaya Haruaki

    Transgenic mouse strains overexpressing KCNJ8 S422L variant (TGmt) or WT (TGWT) in cardiomyocytes have been created to investigate the influence of KCNJ8 in cardiomyocytes and the JWS-related feature of the S422L variant on the cardiac electrophysiology. … These transgenic mouse strains with distinct ECG changes suggested that the S422L mutation in KCNJ8 gene is not a direct cause of JWS. …

    Journal of Pharmacological Sciences 135(1), 37-43, 2017-09

    IR  Ichushi Web 

  • The role of microglia in the brain  [in Japanese]

    Sanagi Tomomi , Kohsaka Shinichi

    ミクログリアは1919年にその存在が報告されて以来, 様々な実験手技の発展とともにその機能が明らかにされてきた. 近年のミクログリア培養法の確立, ミクログリア特異的抗Iba1抗体の開発はミクログリア研究を大きく進展させ, またIba1トランスジェニックマウスを用いたイメージング研究により, 静止型と思われていた正常脳におけるミクログリアの新たな機能が提示された. 一方, 脳の障害を感知するとミク …

    Japanese Journal of Biological Psychiatry 28(2), 69-75, 2017

    J-STAGE  Ichushi Web 

  • Comprehensive functional genomics using <i>Caenorhabditis elegans</i> as a model organism

    MITANI Shohei

    … With the aid of transgenic methods, we analyzed various biological processes in <i>C. … A combination of these biological resources and techniques will be useful to understand the functions of genes in <i>C. …

    Proceedings of the Japan Academy, Series B 93(8), 561-577, 2017

    J-STAGE  Ichushi Web 

  • Artificial Induction of Associative Olfactory Memory by Optogenetic and Thermogenetic Activation of Olfactory Sensory Neurons and Octopaminergic Neurons in Drosophila Larvae

    Honda Takato , Lee Chi-Yu , Honjo Ken , Furukubo-Tokunaga Katsuo , 本庄 賢 , 古久保 - 徳永 克男

    … Recent development of neurogenetic techniques in fruit flies enables manipulations of neuronal activities in freely behaving animals. … Association of reward and odor stimuli is achieved with the concomitant application of blue light and heat that leads to activation of both sets of neurons in living transgenic larvae. …

    Frontiers in Behavioral Neuroscience (10), 137, 2016-06

    IR 

  • Current status of development and regulation for New Plant Breeding Techniques (NPBTs) in the world  [in Japanese]

    Tabei Yukata

    … <p>New Plant Breeding Techniques (NPBT) are used for genome modification in various plant and animal species. … Here I introduce the outline about 6 technologies which are genome editing, oligonucleotide directed mutagenesis (ODM), cisgenesis/intragenesis, grafting, reduced generation time and recurrent breeding by transgenic male sterility in plant. …

    Regulation of Plant Growth & Development 51(2), 130-137, 2016

    J-STAGE 

  • WUSCHEL-RELATED HOMEOBOX 2 is a transcriptional repressor involved in lateral organ formation and separation in <i>Arabidopsis</i>

    Chung KwiMi , Sakamoto Shingo , Mitsuda Nobutaka , Suzuki Kaoru , Ohme-Takagi Masaru , Fujiwara Sumire

    … In this study, we characterized the function of WUSCHEL-RELATED HOMEOBOX 2 (WOX2) using overexpression, CRES-T, and VP16 fusion techniques. … transgenic plants. …

    Plant Biotechnology 33(4), 245-253, 2016

    J-STAGE 

  • Micro-Computed Tomography for the Quantitative 3-Dimensional Assessment of the Compact Myocardium in the Mouse Embryo

    Merchant Samer S. , Kosaka Yasuhiro , Yost H. Joseph , Hsu Edward W. , Brunelli Luca

    … Non-compaction in transgenic mice has been classically evaluated by measuring the thickness of the compact myocardium through histological techniques involving image analysis of 2-dimensional (D) sections. … The 3D and digital nature of the micro-CT data allowed computation anatomical techniques to be readily applied, which were demonstrated via construction of group atlases and atlas-based descriptive statistics. …

    Circulation Journal 80(8), 1795-1803, 2016

    J-STAGE 

  • Biotechnology and apple breeding in Japan

    Igarashi Megumi , Hatsuyama Yoshimichi , Harada Takeo , Fukasawa-Akada Tomoko

    … Various molecular biological techniques have been employed to make apple breeding easier. … Transgenic technology has facilitated the development of apples with resistance to fungal or bacterial diseases, improved fruit quality, or root stocks with better rooting or dwarfing ability. … Using these techniques, no foreign genes are present in the final product, and some of them show considerable promise for application to apple breeding. …

    Breeding Science 66(1), 18-33, 2016

    J-STAGE 

  • Using silkworms to establish alternative animal models for evaluation of drug-induced tissue injury

    Inagaki Yoshinori , Matsumoto Yasuhiko , Sekimizu Kazuhisa

    techniques or invertebrates, evaluation of ADME and the size of animals are still issues that need to be addressed. … Furthermore, transparent transgenic silkworms expressing GFP have been produced to facilitate continuous analysis without the need to collect hemolymph. …

    Drug Discoveries & Therapeutics 10(1), 40-43, 2016

    J-STAGE  Ichushi Web 

  • Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice

    Sakaguchi Masanori , Kim Karam , Yu Lily Mae Yee , Hashikawa Yoshiko , Sekine Yukiko , Okumura Yuki , Kawano Masako , Hayashi Masanobu , Kumar Deependra , Boyden Edward S. , McHugh Thomas J. , Hayashi Yasunori , 坂口 昌徳

    … Therefore, optogenetic techniques hold great potential to establish causal relationships between specific neuronal circuits and their function in freely moving animals. … We established a transgenic mouse line in which tetracycline trans-activator induces ArchT expression. … By crossing this line with a CaMKIIα-tTA transgenic line, the delivery of light via an implanted optrode inhibits the activity of excitatory CA1 neurons. …

    PLoS ONE 10(6), e0130163, 2015-06

    IR 

  • Review of recent transgenic studies on abiotic stress tolerance and future molecular breeding in potato

    Kikuchi Akira , Huynh Huu Duc , Endo Tsukasa , Watanabe Kazuo

    … Recently, many studies have investigated abiotic stress using transgenic techniques. … Thus, based on the study title and the transgene property, transgenic studies were classified into five categories in this review; … From a practical application perspective, further evaluation of transgenic potato with abiotic stress tolerance is required. …

    Breeding Science 65(1), 85-102, 2015

    J-STAGE 

  • Establishment of tools for neurogenetic analysis of sexual behavior in the silkmoth, Bombyx mori

    Kiya Taketoshi , Morishita Koudai , Uchino Keiro , Iwami Masafumi , Sezutsu Hideki

    … Although transgenic techniques and the GAL4/UAS system are well established in the silkmoth, genetic tools useful for investigating brain function at the neural circuit level have been lacking. …

    PLoS ONE 9(11), e113156, 2014-11-14

    IR 

  • Development of mass rearing techniques for transgenic silkworm containment measures  [in Japanese]

    桑原 伸夫 , 池田 真琴 , 清水 健二 [他]

    生物試料分析 = Journal of analytical bio-science 37(3), 207-212, 2014

  • The application of transgenic techniques for physiological and genetical studies in the silkworm, Bombyx mori  [in Japanese]

    嶋田 透

    生物試料分析 = Journal of analytical bio-science 37(3), 169-175, 2014

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