Search Results 1-20 of 107

  • A New Thiophene-based Aggregation-induced Emission Chemosensor for Selective Detection of Zn<sup>2+</sup> Ions and Its Turn Off

    Musib Dulal , Devi L. Reena , Raza Md Kausar , Chanu S. Binita , Roy Mithun

    ion at physiological pH. The probe exhibited remarkable "TURN-ON" emission in the presence of mobile Zn<sup>2+</sup> … ion. … Q-TOF ESI mass spectral analysis and Job's plot confirm 1:1 complex formation with the binding constant, K<sub>b</sub> …

    Chemistry Letters 49(5), 473-476, 2020


  • A Novel Benzimidazole-Based Fluorescence Probe for Detecting Zinc Ion in Aqueous Solution and Zebrafish

    Yun Jin Yeong , Kim Ahran , Hwang Suh Mi , Yun Dongju , Lee Hyojin , Kim Ki-Tae , Kim Cheal

    … The limit of detection (2.26 µM) for zinc ion is well below the WHO standard (76.0 µM). … The binding mechanism of <b>BIN</b> … with zinc ion was demonstrated by fluorescence, UV-visible, electrospray ionization mass spectroscopy, <sup>1</sup>H NMR titration and calculations. … could be applied to determine zinc ion in water samples and living zebrafish.</p> …

    Bulletin of the Chemical Society of Japan 92(5), 961-966, 2019


  • Molecular dynamics study of free energy profile for dissociation of ligand from CA I active site

    ARWANSYAH MS , KODAMA Koichi , KURNIAWAN Isman , KATAOKA Tatsuki , Kawaguchi Kazutomo , Nagao Hidemi

    … We investigate the binding/dissociation process of ligand molecule from carbonicanhydrase (CA) I carbonic anhydrase (CA) I enzyme by using all-atom moleculardynamics simulation. … The force field parameters of zinc ion in the CA I active site are estimatedby quantum chemical calculations and are summarized in this paper. …

    The Science Reports of Kanazawa University = The Science Reports of Kanazawa University (63), 15-28, 2019

    IR  DOI 

  • Syntheses and Biological Evaluation of Novel Hydroxamic Acid Derivatives Containing Purine Moiety as Histone Deacetylase Inhibitors

    Xu Zhaoxing , Ouyang Leiting , Liu Shuhao , Yang Yongchao , Mai Xi , Liu Bin , Xiong Yuanzhen , Feng Lihuang , Liao Yijing , Zhang Yu , Wang Huanlu

    … in the active site of HDAC2 was similar to positive drug <b>SAHA</b>, which were oriented with the hydroxamic acid towards the catalytic center and formed metal binding with zinc ion.</p> …

    Chemical and Pharmaceutical Bulletin 66(4), 439-451, 2018

    J-STAGE  Ichushi Web 

  • Development and applications of genome editing and fluorescent imaging technologies toward precise epigenome editing  [in Japanese]

    Nomura Wataru

    … <p>Zinc finger (ZF) proteins consist of Cys<sub>2</sub>-His<sub>2</sub>-type modules composed of approximately 30 amino acids that adopt a ββα structure and coordinate a zinc ion. … ZF domains recognizing specific DNA target sequences can substitute for the binding domains of various DNA-modifying enzymes to create designer nucleases, recombinases, and methyltransferases with programmable sequence specificity. …

    MEDCHEM NEWS 27(4), 200-207, 2017


  • Zinc Ion-immobilized Magnetic Microspheres for Enrichment and Identification of Multi-phosphorylated Peptides by Mass Spectrometry

    BAE Se Won , KIM Jae Il , CHOI Inseong , SUNG Jiha , HONG Jong-In , YEO Woon-Seok

    … ZnMMs can utilize the rapid and selective isolation/enrichment of phosphorylated peptides and the subsequent mass spectrometric analysis, given the intrinsic magnetic property of magnetic microspheres and the highly selective binding ability of the binuclear Zn<sup>2+</sup>-dipicolylamine complex to phosphate groups. …

    Analytical Sciences 33(12), 1381-1386, 2017

    J-STAGE  Ichushi Web 

  • Regulatory Mechanism of Mast Cell Activation by Zinc Signaling  [in Japanese]

    Nishida Keigo , Uchida Ryota

    … Recent studies have shown that zinc (Zn) ion can behave as an intracellular signaling molecule and that Zn is involved in mast cell activation. … We also found that the LTCC-mediated Zn wave positively controlled inflammatory cytokine gene induction by enhancing the DNA-binding activity of nuclear factor-kappa B (NF-κB). …

    YAKUGAKU ZASSHI 137(5), 495-501, 2017

    J-STAGE  Ichushi Web 

  • Copper diethyldithiocarbamate as an activator of Nrf2 in cultured vascular endothelial cells

    Fujie Tomoya , Murakami Masaki , Yoshida Eiko , Tachinami Tadashi , Shinkai Yasuhiro , Fujiwara Yasuyuki , Yamamoto Chika , Kumagai Yoshito , Naka Hiroshi , Kaji Toshiyuki , 新開 泰弘 , 熊谷 嘉人

    … However, while copper bis(2-hydroxyethyl)dithiocarbamate activated Nrf2, copper ion, diethyldithiocarbamate ligand with or without zinc or iron failed to exhibit this activity. … It was suggested that Cu10-induced activation of Nrf2 was due to proteasome inhibition as well as binding to Kelch-like ECH-associated protein 1. …

    Journal of biological inorganic chemistry : JBIC 21(2), 263-273, 2016-04


  • Controlling Dicopper Protein Functions

    Fujieda Nobutaka , Itoh Shinobu

    … now, several crystal structures have revealed that these copper proteins share a similar dinuclear copper active site, where each copper ion is ligated by three histidine imidazoles, and binds molecular oxygen in a side-on fashion to form a (µ-η<sup>2</sup>:η<sup>2</sup>-peroxido)dicopper(II) species not only as the dioxygen-adduct in oxy-hemocyanins but also as the key reactive intermediate for the hydroxylation of phenols to catechols (phenolase reaction) and the oxidation of catechols to <i>o</i>-qui …

    Bulletin of the Chemical Society of Japan 89(7), 733-742, 2016


  • Structure-Function Analysis and Development of Inhibitors of Metallo-β-lactamases Conferring Drug Resistance in Bacteria  [in Japanese]

    Yamaguchi Yoshihiro

    … Our studies on MBLs, and especially on IMP-1, focus on understanding the role of Zn(II) ion(s) in the hydrolysis of β-lactam antibiotics and on the detailed structure of the IMP-1 active site in order to develop efficient inhibitors. … The results revealed that the first Zn(II) ion is necessary for the hydrolysis of β-lactam antibiotics while the second Zn(II) ion enhances enzyme activity and structural stability, thus helping the enzyme achieve maximum activity. …

    YAKUGAKU ZASSHI 135(11), 1299-1305, 2015

    J-STAGE  Ichushi Web 

  • Zinc Ion-binding Activity of an Anti-ZnO VHH Antibody, 4F2

    Sasaki Ryosuke , Kitazawa Soichiro , Kitahara Ryo , Nakazawa Hikaru , Tanaka Yoshikazu , Kumagai Izumi , Umetsu Mitsuo , Makabe Koki

    … For this purpose, 4F2, a VHH antibody with high affinity and specificity toward zinc oxide (ZnO) surfaces has been constructed. … Here, we report that 4F2 also has an affinity toward zinc ion (Zn<sup>2+</sup>). … We then determined the binding affinity between 4F2 and Zn<sup>2+</sup> …

    Chemistry Letters 44(10), 1309-1311, 2015


  • Application and Potential of Genome Engineering by Artificial Enzymes  [in Japanese]

    Nomura Wataru

    …   Artificial zinc finger proteins (ZFPs) consist of Cys<sub>2</sub>-His<sub>2</sub>-type modules composed of approximately 30 amino acids that adopt a ββα … structure and coordinate a zinc ion. … ZFPs recognizing specific DNA target sequences can substitute for the binding domains of various DNA-modifying enzymes to create designer nucleases, recombinases, and methylases with programmable sequence specificity. …

    YAKUGAKU ZASSHI 135(3), 405-414, 2015

    J-STAGE  Ichushi Web 

  • Allosteric Regulation of the Ligand-Binding Ability of Zinc Porphyrins with Sterically Bulky Shielding Units by Metal Complexation

    Ninomiya Yoshikazu , Kozaki Masatoshi , Suzuki Shuichi , Okada Keiji

    … An artificial allosteric system composed of a zinc porphyrin moiety as a ligand-binding site, diphenylanthracene moieties as sterically bulky shielding units, and bipyridine terminals as Fe(II) ion-binding sites, was designed, synthesized, and characterized. … Consequently, the ligand-binding ability of the zinc porphyrin unit was allosterically suppressed. …

    Bulletin of the Chemical Society of Japan 87(11), 1195-1201, 2014


  • Effects of Conversion of the Zinc-Binding Motif Sequence of Thermolysin, HEXXH, to That of Dipeptidyl Peptidase III, HEXXXH, on the Activity and Stability of Thermolysin

    MENACH Evans , HASHIDA Yasuhiko , YASUKAWA Kiyoshi , INOUYE Kuniyo

    … Most zinc metalloproteinases have the consensus zinc-binding motif sequence HEXXH, in which two histidine residues chelate a catalytic zinc ion. …

    Bioscience, Biotechnology, and Biochemistry 77(9), 1901-1906, 2013-09-23

    J-STAGE  References (37)

  • Metal preferences of zinc-binding motif on metalloproteases.

    Fukasawa Kayoko M , Hata Toshiyuki , Ono Yukio , Hirose Junzo

    … The zinc in any number of zinc metalloproteases has been substituted by some other divalent cation. … Almost all Co(II)- or Mn(II)-substituted enzymes maintain the catalytic activity of their zinc counterparts. …

    福山大学薬学部研究年報 = Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University (30), 22-22, 2012-12-25


  • Development of a dual functional luminescent sensor for zinc ion based on a peptidic architecture.

    Hirayama Tasuku , Taki Masayasu , Akaoka Kazushi , Yamamoto Yukio

    … A synthetic peptide bearing a lanthanide complex, TbOTZ exhibits a decrease of chromophore fluorescence and a concomitant luminescence enhancement due to sensitized Tb(3+) upon Zn(2+) binding. …

    Bioorganic & medicinal chemistry letters 22(24), 7410-7413, 2012-12-15



    KAMEYAMA Takeshi , MATSUYAMA Tetsuya , YASUOKA Kaori , KAKEGAWA Hisao , SUENAGA Yoshihiro

    … As a result, the porous concrete containing 10% baking bone dust efficiently absorbed lead ion, cadmium ion, copper ion, ferric ion and chromium ion. … Manganese ion, nickel ion and strontium ion were also moderately absorbed in the porous concrete. …

    Journal of Japan Society of Civil Engineers, Ser. B3 (Ocean Engineering) 68(2), I_570-I_575, 2012


  • Comparative study of the different mechanisms for zinc ion stress sensing in two cyanobacterial strains, <i>Synechococcus</i> sp. PCC 7942 and <i>Synechocystis</i> sp. PCC 6803

    Morita Eugene Hayato , Kawamoto Satsuki , Abe Shunnosuke , Nishiyama Yoshitaka , Ikegami Takahisa , Hayashi Hidenori

    … and <i>ziaA</i>, as well as the binding affinities of recombinant SmtB and ZiaR proteins. …

    BIOPHYSICS 8(0), 103-109, 2012


  • In rat dipeptidyl peptidase III, His⁵⁶⁸ is essential for catalysis, and Glu⁵⁰⁷ or Glu⁵¹² stabilizes the coordination bond between His⁴⁵⁵ or His⁴⁵⁰ and zinc ion.

    Fukasawa Kayoko M , Hirose Junzo , Hata Toshiyuki , Ono Yukio

    … Dipeptidyl peptidase (DPP) III is a zinc-dependent exopeptidase that has a unique motif, "HELLGH," as the zinc-binding site. … The replacement of the seven charged amino acid residues with a hydrophobic amino acid around the zinc ion did not cause any significant changes in K(m) values or in the substrate specificity. …

    福山大学薬学部研究年報 = Annual report of the Faculty of Pharmacy & Pharmaceutical Sciences, Fukuyama University (29), 55-56, 2011-12-25


  • A novel kind of radioprotector that targets the zinc binding site of p53  [in Japanese]

    OHYA Soichiro , AOKI Shin , IKEKITA Masahiko , MORITA AKinori , MOHD Zulkefeli , ISEKI Ari , WANG Bing , TANAKA Kaoru , OKAZAKI Haruna , YOSHINO Minako , HOSOI Yoshio

    … On the other hand, it is known that p53 denaturation is induced by dissociation of a zinc ion, which is coordinated to metal ion binding site of p53, and hence we evaluated some zinc chelators for inhibition of p53-dependent apoptosis of irradiated MOLT-4 cells. … As a result, two out of five zinc chelators suppressed the apoptosis. …

    The Japan Radiation Research Society Annual Meeting Abstracts 2011(0), 106-106, 2011


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