Simultaneous Measurement of Change in Intracellular Calcium Ions and Degranulation in Single Intact Mast Cells from Rat Peritoneum

• Kusumoto Akihide

  Faculty of Pharmaceutical Sciences, Kyushu University

• Umeoka Ken-ichi

  Faculty of Pharmaceutical Sciences, Kyushu University

• Toda Shoichi

  Faculty of Pharmaceutical Sciences, Kyushu University

• Hirashima Naohide

  Faculty of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, The University of Tokyo

• Kirino Yutaka

  Faculty of Pharmaceutical Sciences, Kyushu University Faculty of Pharmaceutical Sciences, The University of Tokyo

抄録

To investigate the relationship between intracellular calcium dynamics and degranulation in single intact mast cells, we developed a method for the simultaneous observation of these two events. Rat peritoneal mast cells were doubly stained with two types of fluorescent dyes, quinacrine and Ca-indicator fluo-3. Basic quinacrine was specifically incorporated in secretory granules and the fluorescence intensity was decreased upon degranulation. With emission at 520 nm, the excitation spectra for the two dyes differed so that alternate excitation at 420 and 490 nm made it feasible to measure intracellular Ca²⁺ elevation and degranulation, with the time-resolution of 2 s. Degranulation was always accompanied by a preceding transient rise in intracellular Ca²⁺ yet an increase in intracellular Ca²⁺ did not necessarily induce degranulation. These findings suggest that intracellular Ca²⁺ elevation is a necessary but not sufficient condition for degranulation in intact mast cells.

収録刊行物

• bioimages

  bioimages 3 (1), 1-5, 1995

  日本バイオイメージング学会

キーワード

• mast cell
• intracellular calcium
• degranulation
• compound 48/80

詳細情報

• CRID: 1390574732433966080
• NII論文ID: 10002037427
• NII書誌ID: AA11084187
• DOI: 10.11169/bioimages.3.1
• ISSN: 09192719
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可