Chemical Bleaching Improves the Fluorescence Detection Efficiency of Fungi Possessing Dark-Brown Pigments
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- Kawasaki Yukishige
- 1Mitsubishi Kasei Institute of Life Sciences Institute of Space and Astronautical Sciences
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- Tsuji Takashi
- 1Mitsubishi Kasei Institute of Life Sciences
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- Morimoto Michiko
- Faculty of Science, Toho University
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- Haruyama Jun'ichi
- Institute of Space and Astronautical Sciences
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- Takeshima Seiji
- Faculty of Agriculture, Tamagawa University
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- Mikawa Takashi
- Mitsubishi Chemical Industry
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Abstract
Fungi possessing dark-brown pigments such as plant melanin are difficult to detect by fluorescence microscopic techniques. Pretreatment of the pigmented fungi with oxides or chlorides improved the detection efficiency by the fluorescence microscopic technique. Chemical bleaching was effective for spores of all the fungi studied. The fluorescence intensity of probes for cytoplasmic membranes, nucleic acids, and esterases stained to the spores increased more than ten times by this pretreatment, and became detectable not only with an electronic camera but also with the eye.
Journal
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- bioimages
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bioimages 4 (2), 85-88, 1996
Bioimaging Society
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Details
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- CRID
- 1390289321270566144
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- NII Article ID
- 10002037759
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- NII Book ID
- AA11084187
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- ISSN
- 09192719
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- Text Lang
- en
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed