Chemical Bleaching Improves the Fluorescence Detection Efficiency of Fungi Possessing Dark-Brown Pigments

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Abstract

Fungi possessing dark-brown pigments such as plant melanin are difficult to detect by fluorescence microscopic techniques. Pretreatment of the pigmented fungi with oxides or chlorides improved the detection efficiency by the fluorescence microscopic technique. Chemical bleaching was effective for spores of all the fungi studied. The fluorescence intensity of probes for cytoplasmic membranes, nucleic acids, and esterases stained to the spores increased more than ten times by this pretreatment, and became detectable not only with an electronic camera but also with the eye.

Journal

  • bioimages

    bioimages 4 (2), 85-88, 1996

    Bioimaging Society

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Details

  • CRID
    1390289321270566144
  • NII Article ID
    10002037759
  • NII Book ID
    AA11084187
  • DOI
    10.11169/bioimages.4.85
  • ISSN
    09192719
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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