Effect of lcaria Chemotactic Peptide and Its Analogue on the Intracellular Calcium Changes in Guinea Pig Neutrophils

DOI 1 Citations 35 References

Search this article

Abstract

Chemotaxis of guinea pig neutrophils induced by icaria chemotactic peptide (I-CP) and its analogue [Lys7]I-CP was suppressed by pretreatment of the cells with pertussis toxin (IAP) or removal of extra cellular Ca2+. O2- production by formylmethionyl-leucyl-phenylalanine (FMLP) or I-CP was also suppressed under those conditions. In order to investigate the internal signal given by these peptides, we measured intracellular Ca2+ changes both in neutrophil suspension and in single cells using confocal laser scanning microscopy. Both of these peptides and FMLP induced biphasic increase of intracellular calcium concentration ([Ca2+]i. When neutrophils were pretreated with IAP and subsequently stimulated with I-CP or FMLP, the first peak of [Ca2+]i increase was suppressed while the second peak remained. On the other hand, the [Ca2+]i increase induced by [Lys7]I-CP was almost blocked by IAP treatment. These results suggest that the signal transduction pathway taken by [Lys7]I-CP is different from that of I-CP or FMLP.

Journal

  • bioimages

    bioimages 5 (1), 1-9, 1997

    Bioimaging Society

Citations (1)*help

See more

References(35)*help

See more

Keywords

Details 詳細情報について

  • CRID
    1390004951537367552
  • NII Article ID
    10002038057
  • NII Book ID
    AA11084187
  • DOI
    10.11169/bioimages.5.1
  • ISSN
    09192719
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

Report a problem

Back to top