ビス第四級アンモニウム塩型加水分解酵素モデル

• 竹澤 恒雄

  工学院大学工学部応用化学科

• 依田 由紀

  工学院大学工学部応用化学科

• 小山 裕介

  工学院大学工学部応用化学科

• 大勝 靖一

  工学院大学工学部応用化学科

書誌事項

タイトル別名

• Hydrolase Models of Bis-Quaternary Ammonium Salts
• ビス ダイ4キュウ アンモニウムエンガタ カスイ ブンカイ コウソ モデル キシツ ノ シキベツ
• -Recognition of Substrate
• -基質の識別-

抄録

In this study, amphiphiles possessing two equivalent ammonium groups substituted with one methyl and two dodecyls, and a connecting chain were synthesized; the connecting chain consisting of such hydrophilic group that hydroxyl group and ether linkage were introduced on and into the alkanediyl chain. This paper reports the structural effects of a segment connecting two quaternary ammonium salts on the recognition of amino acids in the hydrolysis of amino acid esters using bis-quaternary ammonium salts as catalysts. The hydrophilic nature of the connecting chain was found to have effect on substrate recognition. A connecting chain with high HLB (Hydrophile-Lipophile Balance) increased the hydrolyzing rate of a hydrophilic substrate but decreased that of a hydrophobic substrate. From the relationship between the rate constant k₂ and Michaelis constant K_m in the hydrolysis of amino acid esters, clearly shows that the uptake process of substrate is a rate-determining step, that is, a substrate is recognized by connecting chain in the uptake step. The connecting chain may thus be considered to form a pocket capable of discerning the hydrophilic nature of a substrate.

収録刊行物

• 日本油化学会誌

  日本油化学会誌 48 (3), 227-233,260, 1999

  公益社団法人 日本油化学会

キーワード

• bis-quaternary ammonium salt
• hydrolase model
• connecting chain
• amino acid ester
• recognition

詳細情報

• CRID: 1390001204091931520
• NII論文ID: 10002278607
• NII書誌ID: AN10512582
• DOI: 10.5650/jos1996.48.227
• ISSN: 18841996
• ISSN: 13418327
• NDL書誌ID: 4685059
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4685059
• Web Site: https://www.jstage.jst.go.jp/article/jos1996/48/3/48_3_227/_pdf
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  • NDL
  • Crossref
  • CiNii Articles
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