Chemiluminescence Method for Determining Adenine after a Reaction with an Alkylglyoxal Compound.

• SATO Naofumi

  Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.

• SHIRAKAWA Kamon

  Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.

• SUGIHARA Keisuke

  Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.

• KANAMORI Tosinori

  Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.

Bibliographic Information

Other Title

• Chemiluminescence Method for Determinin

Abstract

The described chemiluminescence method enables the determination of adenine. In a dimethylformamide solution of the products of a reaction between adenine and an alkylglyoxal derivative in the presence of an acid catalyst, chemiluminescence occurs when a sodium hydroxide solution is added. This method is highly sensitive, is specific to compounds containing adenine, and produces no chemiluminescent products whatsoever in reactions with guanine and other nucleic acid bases other than adenine. The detection range for adenine in this reaction was 1.0× 10^-2 to 1.0×10^-7M, and the detection limit 1.4×10^-8M (7.0×10^-14mol per assay). The combined use with polymerase chemical reaction amplification enabled as little as 10pg of DNA to be detected.

Journal

• Analytical Sciences

  Analytical Sciences 13 (1), 59-65, 1997

  The Japan Society for Analytical Chemistry

Keywords

• Chemiluminescence
• adenine
• nucleic acid base
• alkylglyoxal dimethylacetal
• polymerase chain reaction

Details

• CRID: 1390001204253915776
• NII Article ID: 130003528120
• NII Article ID: 10002410526
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.13.59
• COI: 1:CAS:528:DyaK2sXht1KjtLw%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 4142758
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4142758
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.13.59.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.13.59/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci1985/13/1/13_1_59/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed