Fluorometric Assay of DNA by Its Fluorescent Reaction with Chloroacetic Acid.

• LI Yuan-zong

  Department of Chemistry, Peking University

• LIU Xiao-jing

  Department of Chemistry, Peking University

• LIU Hong-fei

  Department of Chemistry, Peking University

• LIU Hong-tao

  Department of Chemistry, Peking University

• CI Yun-xiang

  Department of Chemistry, Peking University

Bibliographic Information

Other Title

• Fluorometric Assay of DNA by Its Fluore

Abstract

A new fluorescent reaction of DNA with chloroacetic acid in the presence of triethylamine is proposed, and the reaction conditions have been optimized. Based on this fluorescent reaction, a sensitive fluorometric method is described for the determination of DNA and the selective determination of cytosine and dCMP. The detection limits for DNA, cytosine and dCMP were 6.0, 0.4 and 1.4ng/ml, respectively.

Journal

• Analytical Sciences

  Analytical Sciences 13 (2), 251-254, 1997

  The Japan Society for Analytical Chemistry

Keywords

• Fluorescent reaction
• DNA
• cytosine
• dCMP
• chloroacetic acid
• triethylamine

Details

• CRID: 1390282679234572544
• NII Article ID: 130003528165
• NII Article ID: 10002411165
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.13.251
• COI: 1:CAS:528:DyaK2sXis12nu7c%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 4196984
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4196984
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.13.251.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.13.251/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci1985/13/2/13_2_251/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed