An Immobilized Acetylcholinesterase Flow-Injection Conductimetric System for the Determination of Paraoxon Tereza
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- RODRIGUES Tereza Cristina
- Universidade Federal de Uberlândia
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- TUBINO Matthieu
- Instituto de Quimica, Universidade Estadual de Campinas
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- GODINHO Oswaldo E. S.
- Centro Tecnológico, Univ. Fed.
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- NETO Graciliano de Oliveira
- Instituto de Quimica, Universidade Estadual de Campinas
書誌事項
- タイトル別名
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- An Immobilized Acetylcholinesterase Flow-Injection Conductimetric System for the Determination of Paraoxon.
- Immobilized Acetylcholinesterase Flow-I
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A flow-injection conductimetric system procedure is proposed for the determination of paraoxon. The method is based on the inhibition of immobilized acetylcholinesterase in controlled porous glass beads (CPG) with acetylcholine as substrate. The enzymatic reaction is inhibited by a diethyl-p-nitrophenylphosphate (paraoxon) solution. A 1, 1′-trimethylene-bis(4-formylpyridinium bromide)dioxime solution was used to reactivate the enzyme. The correlation between the peak height, for a given acetylcholine concentration, is linear from 1.0×10-7 to 5.0×10-5mol l-1 of paraoxon; therefore, the quantitative limit of detection was about 1.0×10-7mol l-1 of paraoxon. For semi-quantitative purposes, the limit can be considered to be about 1.0×10-8mol l-1. The relative estimated standard deviation obtained using a 1.0×10-3mol l-1 acetylcholine solution and a 5.0×10-6mol l-1 paraoxon solution was 2.2% (n=8).
収録刊行物
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- Analytical Sciences
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Analytical Sciences 13 (3), 423-427, 1997
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204252480384
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- NII論文ID
- 130003416633
- 10002411691
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DyaK2sXjvFSrtbo%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 4231293
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可