Rapid and Sensitive Determination of Cyclosporins by Pyrolysis Mass Spectrometry.

• GHASSEMPOUR Alireza

  Department of Chemistry, Sharif University of Technology

• DANESHFAR Rambod

  Chemistry and Chemical Engineering Research Center of Iran

• MOSSEVI Reza

  Department of Chemistry, Sharif University of Technology

• ARSHADI Mohammad Reza

  Department of Chemistry, Sharif University of Technology

Abstract

Cyclosporin A (CsA) is a potent immunosuppressant widely used in human medicine to prevent rejection of transplanted organs. Total concentration of CsA and its metabolites in human blood was determined by pyrolysis mass spectrometry (pyr-ms). Mass to charge (m/z) values of 100 of CsA and 3-nitroaniline as an internal standard were used for quantitative measurements by selected ion monitoring (SIM). Results of pyr-ms at 420°C are highly reproducible. Limit of detection (LOD) and linearity were determined to 0.7 ng ml^-1 and 0.7 ng ml^-1 - 100 mg ml^-1 , respectively. LOD is improved to 0.02 ng ml^-1 by using a solid phase extraction (SPE) disk. Results of pyr-ms are compared with those obtained by the high performance liquid chromatography (HPLC) measurements.

Journal

• Analytical Sciences

  Analytical Sciences 15 (5), 457-460, 1999

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204258315008
• NII Article ID: 130004440066
• NII Article ID: 10002419737
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.15.457
• COI: 1:CAS:528:DyaK1MXjtFeqtLY%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 4721803
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4721803
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.15.457.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.15.457/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/15/5/15_5_457/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed