Electically Stimulated Modulation of Cellular Function in Proliferation,Differentiation,and Gane Expression

• AIZAWA Masuo

  Department of Bioengineering, Tokyo Institute of Technology

• KOYAMA Sumihiro

  Department of Bioengineering, Tokyo Institute of Technology

• KIMURA Keisei

  Department of Bioengineering, Tokyo Institute of Technology

• HARUYAMA Tetsuya

  Department of Bioengineering, Tokyo Institute of Technology

• YANAGIDA Yasuko

  Department of Bioengineering, Tokyo Institute of Technology

• KOBATAKE Eiry

  Department of Bioengineering, Tokyo Institute of Technology

書誌事項

タイトル別名

• Electrically Stimulated Modulation of Cellular Function in Proliferation, Differentiation, and Gene Expression
• Electically Stimulated Modulation of Cellular Function in Proliferation Differentiation and Gene Expression

抄録

The electric modulation of proliferation and differentiation has been performed by electrically stimulating cells that are cultured on the electrode surface in a controlled culture system. Mammalian cells such as HeLa cells halt proliferation at about +0.4V vs. Ag/AgCl without any fatal effects, and proliferate at a potential-dependent rate in the range from 0 to+0.4V vs. Ag/AgCl. Cells are cultured under a controlled rate of proliferation by setting the electrode potential at constant. When the electrode potential is modulated by low frequency of sine wave, the intracellular gene expression is activated, A resting stage of astroglial cells, halting excretion of nerve growth factor (NGF), start generating and excreting NGF by 1-hr electric stimulation at 10Hz with an amplitude of 0.3V, which results from enhancement of NGF mRNA. Differentiation of PC12 cells is also electrically induced to grow neurites in the absencence of NGF. The mechanisms of these electric effects are discussed.

収録刊行物

• Electrochemistry

  Electrochemistry 67 (2), 118-125, 1999-02-05

  公益社団法人　電気化学会

キーワード

• Electrochemical Modulation
• Gene Expression
• Proliferation
• Differentiation

詳細情報

• CRID: 1390001277393463168
• NII論文ID: 10002566260
• NII書誌ID: AN00151637
• DOI: 10.5796/electrochemistry.67.118
• ISSN: 21862451
• ISSN: 13443542
• NDL書誌ID: 4646493
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4646493
• Web Site: https://www.jstage.jst.go.jp/article/electrochemistry/67/2/67_118/_pdf
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用可