Design and Characterization of Polymer Matrix Anchored to Gold Thin Layer for the Effective Usage of Evanescent Field in SPR-based Immunosensor

• Toyama Shigeru

  Research Institute, National Rehabilitation Center for the Disabled

• Ikariyama Yoshihito

  Research Institute, National Rehabilitation Center for the Disabled

抄録

A remarkably stable antigen immobilization on gold thin layer deposited on a glass using aminoethanethiol and oxidized dextran hydrosulfate matrix is reported. The polymer was thus covalently bound onto the gold thin layer, and human serum albumin (HSA) as an antigen was covalently bound on the polymer matrix. The association and the dissociation of anti-HSA (aHSA) with the bound HSA were repeatedly observed by surface plasmon resonance (SPR) measurement. The SPR signal vs. concentration of aHSA has a good experimental agreement with the Langmuir adsorption isotherm equation of the aHSA binding with HSA.

収録刊行物

• Chemistry Letters

  Chemistry Letters 26 (10), 1083-1084, 1997

  公益社団法人 日本化学会

キーワード

• General Chemistry

詳細情報

• CRID: 1390001204576475520
• NII論文ID: 10002616283
• NII書誌ID: AA00603318
• DOI: 10.1246/cl.1997.1083
• COI: 1:CAS:528:DyaK2sXms1egsbk%3D
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/26/10/1083/56073925/cl.1997.1083.pdf
• 本文言語コード: en
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  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可