Micro-behavior and Injury of Biological Cell during Thawing Process

DOI Web Site 2 Citations 13 References
  • TADA Yukio
    Department of Human and Mechanical Systems Engineering, Kanazawa University
  • MOMOSE Noboru
    Department of Mechanical Engineering, Toyama Prefectural University
  • JIANG Rong
    Graduate School of Technology, Kanazawa University
  • HAYASHI Yujiro
    Department of Human and Mechanical Systems Engineering, Kanazawa University

Bibliographic Information

Other Title
  • 細胞の解凍過程におけるミク口挙動と障害
  • 細胞の解凍過程におけるミクロ挙動と障害
  • サイボウ ノ カイトウ カテイ ニ オケル ミクロ キョドウ ト ショウガイ

Search this article

Abstract

This study has been conducted to pursue the relation between microscale behavior and the injury of biological cell during freezing and thawing. As a sample of biological cells, protoplasts isolated from cultured wheat cells were selectively used. As the results of microscopic observation using a cold stage whose cooling and heating velocities were controlled, the recovery of cell by water influx due to osmotic pressure difference, and the fusion of intracellular ice were clarified with heating velocity. It was found that the osmotic stress acting on the ce11 membrane causes the thawing injuries connecting with swell and rupture of cell. The survival of cells was also inspected by dye-exclusion test using Evans Blue. The results suggested rapid temperature-rising is more harmful for slowly-frozen cell.

Journal

Citations (2)*help

See more

References(13)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top