精密重合・精密合成高分子Ｉ　　バイオテクノロジーによる精密重合の展開　遺伝子工学の拡張と進化分子工学

• 伊藤 嘉浩

  奈良先端科学技術大学院大学・物質創成科学研究科 科学技術振興事業団さきがけ研究21

書誌事項

タイトル別名

• Precision Polymerization and Polymers I. Biotechnology for Precision Polymerization-Gene Technology, Expanded Gene Technology, and Molecular Evolution Engineering.
• バイオテクノロジー ニヨル セイミツ ジュウゴウ ノ テンカイ イデンシ コウ

抄録

Methodologies for precision polymerization based on biotechnology are reviewed. Gene technology was used for incoporation of polyproline residues of various lengths into the carboxyl terminal of enzymes. The mutant enzymes were hydrophobic and had functions which were different from those of wild-type enzymes. Expanded gene technology for incorporation of nonnatural amino acids into proteins is also described. Several synthetic methods of misacylated tRNA for the purpose are reported. Molecular evolution engineering, in vitro selection, is reviewed which utilizes an enzymatic polymerization method, polymerase chain reaction (PCR). The future progress using nonnatural nucleic acids is discussed.

収録刊行物

• 高分子論文集

  高分子論文集 54 (10), 596-607, 1997

  公益社団法人 高分子学会

キーワード

• Gene Engineering
• Protein Engineering
• Molecular Evolution Engineering
• Nonnatural Amino Acid
• Nonnatural Nucleic Acid

詳細情報

• CRID: 1390282681500236672
• NII論文ID: 10003286693
• NII書誌ID: AN00085011
• DOI: 10.1295/koron.54.596
• ISSN: 18815685
• ISSN: 03862186
• ISSN: http://id.crossref.org/issn/03862186
• NDL書誌ID: 4334396
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4334396
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可