Precision Polymerization and Polymers I. Biotechnology for Precision Polymerization-Gene Technology, Expanded Gene Technology, and Molecular Evolution Engineering.

• ITO Yoshihiro

  Graduate School of Materials Science, Nara Institute of Science and Technology Japan Science and Technology Corporation

Bibliographic Information

Other Title

• 精密重合・精密合成高分子Ｉ　　バイオテクノロジーによる精密重合の展開　遺伝子工学の拡張と進化分子工学
• バイオテクノロジー ニヨル セイミツ ジュウゴウ ノ テンカイ イデンシ コウ

Abstract

Methodologies for precision polymerization based on biotechnology are reviewed. Gene technology was used for incoporation of polyproline residues of various lengths into the carboxyl terminal of enzymes. The mutant enzymes were hydrophobic and had functions which were different from those of wild-type enzymes. Expanded gene technology for incorporation of nonnatural amino acids into proteins is also described. Several synthetic methods of misacylated tRNA for the purpose are reported. Molecular evolution engineering, in vitro selection, is reviewed which utilizes an enzymatic polymerization method, polymerase chain reaction (PCR). The future progress using nonnatural nucleic acids is discussed.

Journal

• KOBUNSHI RONBUNSHU

  KOBUNSHI RONBUNSHU 54 (10), 596-607, 1997

  The Society of Polymer Science, Japan

Keywords

• Gene Engineering
• Protein Engineering
• Molecular Evolution Engineering
• Nonnatural Amino Acid
• Nonnatural Nucleic Acid

Details

• CRID: 1390282681500236672
• NII Article ID: 10003286693
• NII Book ID: AN00085011
• DOI: 10.1295/koron.54.596
• ISSN: 18815685
• ISSN: 03862186
• ISSN: http://id.crossref.org/issn/03862186
• NDL BIB ID: 4334396
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4334396
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed