Polymerase chain reaction -single strand conformational polymorphism analysis of intra- and interspecific variations in organellar DNA regions of Aegilops mutica and related species

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In order to study the phylogeny of <i>Aegilops mutica</i> in the genera of <i>Triticum</i> and <i>Aegilops</i>, variations in chloroplast and mitochondrial DNA regions were investigated by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis. Nine lines, each of <i>Ae. mutica </i>and <i>Ae. speltoides</i>, were studied together with nine other <i>Triticum</i> and <i>Aegilops</i> species, including <i>T. aestivum</i>. By analyzing 9.7-kb chloroplast and 13.1-kb mitochondrial DNA regions, a total of 268 bands were detected, of which 176 (65.7%) showed variation within and/or between species. The level of intraspecific variation of <i>Ae. mutica</i> was lower than that of <i>Ae. speltoides</i>. The low level of the intraspecific variation of <i>Ae. mutica</i> was contrary to the expectation from previous studies on morphological and cytolo-gical characters. In the phylogenetic trees based on SSCP, <i>Ae. mutica</i>, <i>Ae. speltoides</i> and the other four species of the section Sitopsis (the subsection Emarginata) were separated into three different clusters. In addition, <i>T. aestivum</i> was included in the cluster of <i>Ae. speltoides</i> in the phylogenetic trees. This result suggests that <i>Ae. speltoides</i> is the cytoplasmic donor of common wheat.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 71(5), 281-292, 1996-10

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003760153
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4163732
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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