Quantitative chromosome mapping of small plant chromosomes by improved imaging on CHIAS II

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Improved imaging procedures were developed and employed for the construction of a quantitative chromosome map based on condensation pattern. The condensa-tion pattern is uneven condensation along a chromosomal axis resulting from differential condensation of chromatin fibers at prometaphase appearing commonly in small plant chromosomes, represented by the genus <i>Brassica</i>. <i>Brassica</i> chromosomes have distinct condensation patterns at the prometaphase stage. The procedures for quantitative mapping are divided into six parts: (i) capture of images, (ii) adjustment of light distortion, (iii) measurements of an image parameter, CP, or density profiles and length of chromosomal arms, (iv) gray value adjustments between homologous chromosomes, (v) adjustment of chromosomal lengths and obtaining the standard CP, or graygram, and (vi) thresholding graygram for a quantitative chromosome map or an idiogram. The quantitative chromosome map shows well the characteristics of the <i>Brassica</i> chromosomes. The series of imaging procedures enable construction of quantitative chromosome maps and can be employed for a wide range of species having various chromosome numbers. The quantitative chromosome map constructed by the improved imaging currently developed is an aid to cytogenetical and genome analysis of plant species.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 72(1), 35-40, 1997-02

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003760588
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4196967
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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