Identification of the sex of Oriental white stork, Ciconia boyciana, by the polymerase chain reaction based on its sex chromosome-specific DNA sequences

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Southern blotting of <i>Hin</i>dIII-digested genomic DNAs from the female and male Oriental white stork (<i>Ciconia boyciana</i>) with the EE0.6 probe cloned from the W chromosome of chicken (<i>Gallus domesticus</i>) produced a 3.0-kb W chromosome-derived band specific to the female and a 3.5-kb Z chromosome-derived band common to both sexes. These two genomic fragments were cloned and the counter-part sequences to that of EE0.6 in these fragments, XH0.6 and XH0.6RSM (XH0.6-related sequence in the male), respectively, were subcloned. Nucleotide sequences of XH0.6 and XH0.6RSM showed 92% identity. PCR using a set of primer sequences from XH0.6, which differed several nucleotides from those in XH0.6RSM, amplified an about 300-bp genomic sequence only from the female <i>C. boyciana</i>. This method was applied successfully to identify the sex of individual young birds of <i>C. boyciana</i>, an endangered special natural monument in Japan and whose sexes are unidentifiable from their external morphology.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 72(1), 51-56, 1997-02

    The Genetics Society of Japan

参考文献:  8件中 1-8件 を表示

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各種コード

  • NII論文ID(NAID)
    10003760639
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4196969
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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