A nuclear compatibility gene, Ncc-tmp, of Triticum timopheevi for the cytoplasm of Aegilops squarrosa.

• Asakura Nobuaki

  Graduate School of Science and Technology, Kobe University

• Nakamura Chiharu

  Graduate School of Science and Technology, Kobe University Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, Kobe University

• Ohtsuka Ichiro

  Laboratory of Biology, Faculty of Engineering, Kanagawa University

Bibliographic Information

Other Title

• nuclear compatibility gene Ncc-tmp of T

Abstract

A nuclear genome of timopheevi wheat is compatible with the cytoplasm of Aegilops squarrosa, while that of durum wheat is incompatible, thus no euploid hybrids with this cytoplasm could be obtained. A euploid nucleus-cytoplasm (NC) hybrid of Triticum durum cv. Langdon was produced by introgressing a NC compatibility gene (Ncc-tmp) of T. timopheevi. Southern blot analysis, using one RAPD marker (S13₈₃₀) that is tightly linked to Ncc-tmp and DNAs from a series of D genome chromosome substitution lines of Langdon, demonstrated that Ncc-tmp locates on chromosome 1A. A similar function of Ncc-tmp and Ncc-sqr of Ae. squarrosa for its own cytoplasm suggested that they are homoeoallelic. Homologous sequences of two other linked RAPD markers (R03₈₂₀ and R19₆₀₀) were detected in all accessions of T. urartu but not in T. boeoticum. The result showed the conservation of sequences adjacent to Ncc-tmp in T. urartu, an A genome donor for emmer and timopheevi wheat. An analysis of γ-gliadin showed that the NC hybrid possesses one allele that might have been introgressed from chromosome 1G of T. timopheevi. Together with this result, 3:1 segregation of plump and shriveled seeds in BC₁ of the cross between (sqr)-T. timopheevi and T. turgidum suggested the presence of an additional and functional homoeoallelic Ncc-tmp in T. timopheevi.<br>

Journal

• Genes & Genetic Systems

  Genes & Genetic Systems 72 (2), 71-78, 1997

  The Genetics Society of Japan

Keywords

• Genetics
• Molecular Biology
• General Medicine

Details

• CRID: 1390282680420532352
• NII Article ID: 10003760699
• NII Book ID: AA11077421
• DOI: 10.1266/ggs.72.71
• COI: 1:CAS:528:DyaK2sXlsFCltLg%3D
• ISSN: 18805779
• ISSN: 13417568
• NDL BIB ID: 4240360
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4240360
• Web Site: http://www.jstage.jst.go.jp/article/ggs/72/2/72_2_71/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed