Detection of the Sec-1 locus of rye by a PCR-based method

この論文にアクセスする

この論文をさがす

著者

抄録

The structural genes for the ω-secalins of rye (<i>Secale cereale</i>) are located in the <i>Sec-1</i> locus on the short arm of rye chromosome 1R. We applied PCR (polymerase chain reaction) to detect the <i>Sec-1</i> locus in a wheat genomic background. A primer set we designed based on a published sequence of a ω-secalin gene amplified not only the ω-secalin sequence, but also a putative ω-gliadin sequence. We determined partial sequences of both PCR-amplified fragments and designed different primers for the specific amplification of the ω-secalin sequence. One of the new primer sets amplified DNA fragments only in rye and wheat lines carrying chromosome 1R or telosome 1RS; no amplification occurred in either euploid wheats or 1RS deletion lines. This PCR-based method would provide efficient screening for the <i>Sec-1</i> locus in progeny of wheat lines carrying chromosome 1R.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 72(4), 197-203, 1997-08

    The Genetics Society of Japan

参考文献:  19件中 1-19件 を表示

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10003761229
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4326603
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ