Detection of the Sec-1 locus of rye by a PCR-based method.
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- Shimizu Yuichiro
- Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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- Nasuda Shuhei
- Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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- Endo Takashi Ryu
- Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- Detection of the Sec-1 locus of rye by
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Abstract
The structural genes for the ω-secalins of rye (Secale cereale) are located in the Sec-1 locus on the short arm of rye chromosome 1R. We applied PCR (polymerase chain reaction) to detect the Sec-1 locus in a wheat genomic background. A primer set we designed based on a published sequence of a ω-secalin gene amplified not only the ω-secalin sequence, but also a putative ω-gliadin sequence. We determined partial sequences of both PCR-amplified fragments and designed different primers for the specific amplification of the ω-secalin sequence. One of the new primer sets amplified DNA fragments only in rye and wheat lines carrying chromosome 1R or telosome 1RS; no amplification occurred in either euploid wheats or 1RS deletion lines. This PCR-based method would provide efficient screening for the Sec-1 locus in progeny of wheat lines carrying chromosome 1R.<br>
Journal
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- Genes & Genetic Systems
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Genes & Genetic Systems 72 (4), 197-203, 1997
The Genetics Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001205445950976
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- NII Article ID
- 10003761229
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- NII Book ID
- AA11077421
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- COI
- 1:CAS:528:DyaK1cXisVSjsw%3D%3D
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- ISSN
- 18805779
- 13417568
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- NDL BIB ID
- 4326603
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- PubMed
- 9418259
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed