Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes

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In the medaka, <i>Oryzias latipes</i>, which does not have cytologically recognizable sex chromosomes, the mechanism of sex determination (XX/XY) can be revealed by genetic crosses using a particular pigment gene. Since the only known sex-linked marker is this pigment gene, little information is available on the genetic maps of the medaka's sex chromosomes. High resolution genetic maps of its sex chromosomes are necessary to hunt for the sex-determining factor by the positional cloning method. In the present study, we isolated a sex-linked marker using the genomic differences between inbred strains of medaka. We established a congenic strain whose sex-determining region is derived from the HNI strain and whose genetic background is derived from the Hd-rR strain. Using differences between the genomes of the congenic and Hd-rR strains, we isolated a sex-linked clone (pHO5.5) from a random genomic library constructed from the HO5 strain of medaka. The pHO5.5-related sequences were conserved among <i>Oryzias</i> species. A linkage analysis using backcross progeny from the Hd-rR and HNI strains demonstrated perfect linkage between the pHO5.5-related sequence and the sex. We, hence, designated the locus of the pHO5.5-related sequence on sex chromosomes of medaka as <i>S</i>ex-<i>L</i>inked <i>1</i> (<i>SL1</i>). Using this sequence, we could identify the sex of inbred strains of medaka by PCR or Southern blotting. This sequence may be useful for genetic mapping on the sex chromosomes. We also discuss the availability of the congenic strain established in this study for isolating other sex-linked clones.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 72(5), 263-268, 1997-10

    The Genetics Society of Japan

参考文献:  17件中 1-17件 を表示

被引用文献:  3件中 1-3件 を表示

各種コード

  • NII論文ID(NAID)
    10003761462
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4474102
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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