Wheat MADS box genes, a multigene family dispersed throughout the genome

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Polymerase chain reaction (PCR) with degenerate primers was utilized for partial cloning of the MADS box gene family from wheat (<i>Triticum aestivum</i> L.). PCR products corresponding to a part of the MADS box region were cloned and sequenced. Eleven individual clones sequenced were classified into seven types on the basis of the nucleotide sequence and five types on the deduced amino acid sequence, which included two wheat-specific MADS box protein sequences. RT-PCR analysis with degenerate primers revealed preferential expression of the MADS box genes in young spikes. Furthermore, genomic Southern blot analysis with degenerate PCR products as probes indicated that wheat MADS box genes constitute a multigene family and are dispersed throughout the genome.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 72(5), 317-321, 1997-10

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003761688
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13417568
  • NDL 記事登録ID
    4474110
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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