Dissection of chromosome region 89A of Drosophila melanogaster by local transposition of P elements

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In the chromosome region 89A of <i>Drosophila melanogaster</i>, a few meiotic genes have been suggested to exist besides <i>c3G</i> and <i>rec</i>. We carried out local mutagenesis using a strain carrying a <i>P</i> element-insertion (plwB) at 89A, and obtained new genetic variants. Two are female sterile mutations, an allele of the <i>homeless</i> locus (<i>hls</i><sup>167</sup>) and a new mutation <i>tibi</i> (<i>tbi</i>), and three are lethal mutations at the <i>serpent</i> locus. The <i>tbi</i> mutation is a paternally-rescuable maternal-effect-lethal. Destabilization of the <i>P</i> elements revealed that these mutations were caused by <i>P</i> element-insertions, and produced 12 deletion lines. These lines were then used for systematic complementation tests. The results showed that: (1) <i>hls</i>, <i>tbi</i> and at least three lethal genes in addition to <i>c3G</i>, <i>rec</i> and <i>l(3)89Aa</i> are located within the deletion of <i>Df(3R)c3G2</i> (89A2-3; 89A4-5); (2) the gene order is <i>rec</i>, <i>tbi</i>, <i>hls</i> (from centromere to telomere), and both <i>c3G</i> and <i>l(3)89Aa</i> are possibly located proximally. We cloned 117 kb of DNA from this region by plasmid rescue and chromosome walking, and mapped several of the breakpoints of the deletions. These analyses delimited the <i>rec</i> gene to within 21 kb of the cloned DNA, although the <i>c3G</i> gene could not be located on the molecular map.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 73(2), 95-103, 1998-04

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003762240
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4514154
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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