Detection of terminal deletions in barley chromosomes by the PCR-based method

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Barley chromosomes have barley-specific repetitive sequences (HvT01) in the subtelomeric regions. The subtelomeric repeats were amplified by the polymerase chain reaction (PCR) in 11 wheat-barley telosome addition lines, but no or little amplification occurred in the euploid common wheat cultivar `Chinese Spring'. A gametocidal chromosome 2C, derived from an <i>Aegilops cylindrica</i> chromosome, induces chromosome breaks in Chinese Spring. To explore the potential of using PCR as a screen for barley telosomes with terminal deletions, induced by chromosome 2C, the progeny of a barley ditelosomic (6HS) addition line of Chinese Spring carrying chromosome 2C was investigated. The progeny plants in which DNA amplification occurred by PCR always had a normal 6HS whereas those in which no DNA amplification was observed carried either no 6HS or a 6HS telosome with a deletion. In this work the PCR based assay described is shown to be a robust and reliable method of identifying terminal deletions in barley telosomes in a Chinese Spring background.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 73(3), 163-166, 1998-06

    The Genetics Society of Japan

参考文献:  17件中 1-17件 を表示

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各種コード

  • NII論文ID(NAID)
    10003762527
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4549977
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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